Adds KRAKEN_COMBINEKREPORTS for 'native' multi-sample OTU

conf/modules.config

@@ -294,6 +294,15 @@ process {
         ]
     }
 
+    withName: KRAKENTOOLS_COMBINEKREPORTS {
+        ext.prefix = { "${meta.id}_combined_reports" }
+        publishDir = [
+            path: { "${params.outdir}/kraken2/" },
+            mode: params.publish_dir_mode,
+            pattern: '*.{txt}'
+        ]
+    }
+
     withName: KRONA_CLEANUP {
         ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" }
         publishDir = [

modules.json

@@ -73,6 +73,10 @@
                     "branch": "master",
                     "git_sha": "409a308ba46284d8ebb48c2c1befd6f6433db3f7"
                 },
+                "krakentools/combinekreports": {
+                    "branch": "master",
+                    "git_sha": "ee0346b4d14ffdc15ce7e093ca1363cd07c9bd78"
+                },
                 "krakentools/kreport2krona": {
                     "branch": "master",
                     "git_sha": "233fa70811a03a4cecb2ece483b5c8396e2cee1d"

modules/nf-core/modules/krakentools/combinekreports/main.nf

@@ -0,0 +1,34 @@
+process KRAKENTOOLS_COMBINEKREPORTS {
+    label 'process_low'
+
+    conda (params.enable_conda ? "bioconda::krakentools=1.2" : null)
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/krakentools:1.2--pyh5e36f6f_0':
+        'quay.io/biocontainers/krakentools:1.2--pyh5e36f6f_0' }"
+
+    input:
+    tuple val(meta), path(kreports)
+
+    output:
+    tuple val(meta), path("*.txt"), emit: txt
+    path "versions.yml", emit: versions
+
+    when:
+    task.ext.when == null || task.ext.when
+
+    script:
+    def args = task.ext.args ?: ''
+    prefix = task.ext.prefix ?: "${meta.id}"
+    def VERSION = '1.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions.
+    """
+    combine_kreports.py \\
+        -r ${kreports} \\
+        -o ${prefix}.txt \\
+        ${args}
+
+    cat <<-END_VERSIONS > versions.yml
+    "${task.process}":
+        combine_kreports.py: ${VERSION}
+    END_VERSIONS
+    """
+}

modules/nf-core/modules/krakentools/combinekreports/meta.yml

@@ -0,0 +1,43 @@
+name: krakentools_combinekreports
+description: Takes a Kraken report file and prints out a krona-compatible TEXT file
+keywords:
+  - kraken
+  - krakentools
+  - metagenomics
+  - table
+  - combining
+  - merging
+tools:
+  - krakentools:
+      description: KrakenTools is a suite of scripts to be used for post-analysis of Kraken/KrakenUniq/Kraken2/Bracken results. Please cite the relevant paper if using KrakenTools with any of the listed programs.
+      homepage: https://github.com/jenniferlu717/KrakenTools
+      licence: ["GPL v3"]
+
+input:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - kreports:
+      type: file
+      description: List of kraken-style report files
+      pattern: "*.{txt,kreport}"
+
+output:
+  - meta:
+      type: map
+      description: |
+        Groovy Map containing sample information
+        e.g. [ id:'test', single_end:false ]
+  - versions:
+      type: file
+      description: File containing software versions
+      pattern: "versions.yml"
+  - txt:
+      type: file
+      description: Combined kreport file of all input files
+      pattern: "*.txt"
+
+authors:
+  - "@jfy133"

subworkflows/local/standardisation_profiles.nf

@@ -3,7 +3,8 @@
 //
 
 include { KAIJU_KAIJU2TABLE                     } from '../../modules/nf-core/modules/kaiju/kaiju2table/main'
-include { MOTUS_MERGE } from '../../modules/nf-core/modules/motus/merge/main'
+include { KRAKENTOOLS_COMBINEKREPORTS           } from '../../modules/nf-core/modules/krakentools/combinekreports/main'
+include { MOTUS_MERGE                           } from '../../modules/nf-core/modules/motus/merge/main'
 
 workflow STANDARDISATION_PROFILES {
     take:
@@ -23,6 +24,7 @@ workflow STANDARDISATION_PROFILES {
     ch_input_profiles = profiles
         .branch {
             motus: it[0]['tool'] == 'motus'
+            kraken2: it[0]['tool'] == 'kraken2'
             unknown: true
         }
 
@@ -58,6 +60,21 @@ workflow STANDARDISATION_PROFILES {
     ch_multiqc_files = ch_multiqc_files.mix( KAIJU_KAIJU2TABLE.out.summary )
     ch_versions = ch_versions.mix( KAIJU_KAIJU2TABLE.out.versions )
 
+    // Kraken2
+
+    // Collect and replace id for db_name for prefix
+    ch_profiles_for_kraken2 = ch_input_profiles.kraken2
+                                .map { [it[0]['db_name'], it[1]] }
+                                .groupTuple(sort: {-it.size()} )
+                                .map {
+                                    [[id:it[0]], it[1]]
+                                }
+
+    KRAKENTOOLS_COMBINEKREPORTS ( ch_profiles_for_kraken2 )
+    ch_standardised_tables = ch_standardised_tables.mix( KRAKENTOOLS_COMBINEKREPORTS.out.txt )
+    ch_multiqc_files = ch_multiqc_files.mix( KRAKENTOOLS_COMBINEKREPORTS.out.txt )
+    ch_versions = ch_versions.mix( KRAKENTOOLS_COMBINEKREPORTS.out.versions )
+
     // mOTUs has a 'single' database, and cannot create custom ones.
     // Therefore removing db info here, and publish merged at root mOTUs results
     // directory