diff --git a/docs/usage.md b/docs/usage.md index 0be44ed..5769bd9 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -440,8 +440,24 @@ Kraken2 allows the user to build custom databases. You can follow Kraken2 [tutor Centrifuge is similar to Kraken2 in that it enables to [build custom databases](https://ccb.jhu.edu/software/centrifuge/manual.shtml#custom-database). #### Kaiju -WIP +It is possible to [create custom databases](https://github.com/bioinformatics-centre/kaiju#custom-database) with Kaiju. +#### MALT +To create a custom database for MALT, the user should download and unzip the following database which lists all NCBI records. The input files are specified using -i and the index is specified using -d. A detailed description for each argument can be found [here](https://software-ab.informatik.uni-tuebingen.de/download/malt/manual.pdf) + +```bash +wget https://software-ab.informatik.uni-tuebingen.de/download/megan6/megan-nucl-Feb2022.db.zip +unzip megan-nucl-Feb2022.db +malt-build -i path/to/fasta/files/*.{fna,fa} -s DNA -d index -t 8 -st 4 -a2t megan-nucl-Feb2022.db +``` + +#### Bracken + +#### KrakenUniq + +#### DIAMOND + +#### mOTUs ## Troubleshooting and FAQs