// // Process short raw reads with FastP // include { FASTP as FASTP_SINGLE } from '../../modules/nf-core/modules/fastp/main' include { FASTP as FASTP_PAIRED } from '../../modules/nf-core/modules/fastp/main' workflow SHORTREAD_FASTP { take: reads // [[meta], [reads]] main: ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() ch_input_for_fastp = reads .branch{ single: it[0]['single_end'] == true paired: it[0]['single_end'] == false } FASTP_SINGLE ( ch_input_for_fastp.single, false, false ) // Last parameter here turns on merging of PE data FASTP_PAIRED ( ch_input_for_fastp.paired, false, params.shortread_qc_mergepairs ) if ( params.shortread_qc_mergepairs ) { ch_fastp_reads_prepped_pe = FASTP_PAIRED.out.reads_merged .map { meta, reads -> def meta_new = meta.clone() meta_new['single_end'] = true [ meta_new, [ reads ].flatten() ] } ch_fastp_reads_prepped = ch_fastp_reads_prepped_pe.mix( FASTP_SINGLE.out.reads ) } else { ch_fastp_reads_prepped = FASTP_PAIRED.out.reads .mix( FASTP_SINGLE.out.reads ) } ch_versions = ch_versions.mix(FASTP_SINGLE.out.versions.first()) ch_versions = ch_versions.mix(FASTP_PAIRED.out.versions.first()) ch_processed_reads = ch_fastp_reads_prepped ch_multiqc_files = ch_multiqc_files.mix( FASTP_SINGLE.out.json ) ch_multiqc_files = ch_multiqc_files.mix( FASTP_PAIRED.out.json ) emit: reads = ch_processed_reads // channel: [ val(meta), [ reads ] ] versions = ch_versions // channel: [ versions.yml ] mqc = ch_multiqc_files }