/* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ VALIDATE INPUTS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) // Validate input parameters WorkflowTaxprofiler.initialise(params, log) // TODO nf-core: Add all file path parameters for the pipeline to the list below // Check input path parameters to see if they exist def checkPathParamList = [ params.input, params.databases, params.multiqc_config ] for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } // Check mandatory parameters if (params.input ) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } if (params.databases) { ch_databases = file(params.databases) } else { exit 1, 'Input database sheet not specified!' } if (params.shortread_clipmerge_mergepairs && params.run_malt ) log.warn "[nf-core/taxprofiler] warning: MALT does not accept uncollapsed paired-reads. Pairs will be profiled as separate files." if (params.shortread_clipmerge_excludeunmerged && !params.shortread_clipmerge_mergepairs) exit 1, "[nf-core/taxprofiler] error: cannot include unmerged reads when merging not turned on. Please specify --shortread_clipmerge_mergepairs" /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ CONFIG FILES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ ch_multiqc_config = file("$projectDir/assets/multiqc_config.yml", checkIfExists: true) ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT LOCAL MODULES/SUBWORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // include { INPUT_CHECK } from '../subworkflows/local/input_check' include { DB_CHECK } from '../subworkflows/local/db_check' include { SHORTREAD_PREPROCESSING } from '../subworkflows/local/shortread_preprocessing' include { LONGREAD_PREPROCESSING } from '../subworkflows/local/longread_preprocessing' include { SHORTREAD_COMPLEXITYFILTERING } from '../subworkflows/local/shortread_complexityfiltering' include { PROFILING } from '../subworkflows/local/profiling' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT NF-CORE MODULES/SUBWORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // // MODULE: Installed directly from nf-core/modules // include { FASTQC } from '../modules/nf-core/modules/fastqc/main' include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main' include { CAT_FASTQ } from '../modules/nf-core/modules/cat/fastq/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ RUN MAIN WORKFLOW ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // Info required for completion email and summary def multiqc_report = [] workflow TAXPROFILER { ch_versions = Channel.empty() /* SUBWORKFLOW: Read in samplesheet, validate and stage input files */ INPUT_CHECK ( ch_input ) ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) DB_CHECK ( ch_databases ) ch_versions = ch_versions.mix(DB_CHECK.out.versions) /* MODULE: Run FastQC */ ch_input_for_fastqc = INPUT_CHECK.out.fastq.mix( INPUT_CHECK.out.nanopore ) FASTQC ( ch_input_for_fastqc ) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) /* SUBWORKFLOW: PERFORM PREPROCESSING */ if ( params.shortread_clipmerge ) { ch_shortreads_preprocessed = SHORTREAD_PREPROCESSING ( INPUT_CHECK.out.fastq ).reads } else { ch_shortreads_preprocessed = INPUT_CHECK.out.fastq } if ( params.longread_clip ) { ch_longreads_preprocessed = LONGREAD_PREPROCESSING ( INPUT_CHECK.out.nanopore ).reads .map { it -> [ it[0], [it[1]] ] } } else { ch_longreads_preprocessed = INPUT_CHECK.out.nanopore } /* SUBWORKFLOW: COMPLEXITY FILTERING */ if ( params.shortread_complexityfilter ) { ch_shortreads_filtered = SHORTREAD_COMPLEXITYFILTERING ( ch_shortreads_preprocessed ).reads } else { ch_shortreads_filtered = ch_shortreads_preprocessed } /* STEP: Run merging */ if ( params.run_merging ) { ch_reads_for_cat_branch = ch_shortreads_filtered .mix( ch_longreads_preprocessed ) .map { meta, reads -> def meta_new = meta.clone() meta_new.remove('run_accession') [ meta_new, reads ] } .groupTuple(by: 0) .map { meta, reads -> [ meta, reads.flatten() ] } .branch { // we can't concatenate files if there is not a second run, we branch // here to separate them out, and mix back in after for efficiency cat: ( it[0]['single_end'] && it[1].size() > 1 ) || ( !it[0]['single_end'] && it[1].size() > 2 ) skip: true } ch_reads_runmerged = CAT_FASTQ ( ch_reads_for_cat_branch.cat ).reads .mix( ch_reads_for_cat_branch.skip ) .map { meta, reads -> [ meta, [ reads ].flatten() ] } } else { ch_reads_runmerged = ch_shortreads_filtered .mix( ch_longreads_preprocessed ) } /* SUBWORKFLOW: PROFILING */ PROFILING ( ch_reads_runmerged, DB_CHECK.out.dbs ) ch_versions = ch_versions.mix( PROFILING.out.versions ) /* MODULE: MultiQC */ CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) workflow_summary = WorkflowTaxprofiler.paramsSummaryMultiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) ch_multiqc_files = Channel.empty() ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) if (params.shortread_clipmerge) { ch_multiqc_files = ch_multiqc_files.mix( SHORTREAD_PREPROCESSING.out.mqc.collect{it[1]}.ifEmpty([]) ) ch_versions = ch_versions.mix( SHORTREAD_PREPROCESSING.out.versions ) } if (params.longread_clip) { ch_multiqc_files = ch_multiqc_files.mix( LONGREAD_PREPROCESSING.out.mqc.collect{it[1]}.ifEmpty([]) ) ch_versions = ch_versions.mix( LONGREAD_PREPROCESSING.out.versions ) } if (params.shortread_complexityfilter){ ch_multiqc_files = ch_multiqc_files.mix( SHORTREAD_COMPLEXITYFILTERING.out.mqc.collect{it[1]}.ifEmpty([]) ) ch_versions = ch_versions.mix( SHORTREAD_COMPLEXITYFILTERING.out.versions ) } if (params.run_merging){ ch_versions = ch_versions.mix(CAT_FASTQ.out.versions) } ch_multiqc_files = ch_multiqc_files.mix( PROFILING.out.mqc ) // TODO create multiQC module for metaphlan MULTIQC ( ch_multiqc_files.collect() ) multiqc_report = MULTIQC.out.report.toList() ch_versions = ch_versions.mix(MULTIQC.out.versions) } /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ COMPLETION EMAIL AND SUMMARY ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow.onComplete { if (params.email || params.email_on_fail) { NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) } NfcoreTemplate.summary(workflow, params, log) } /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */