name: samtools_fastq description: Converts a SAM/BAM/CRAM file to FASTQ keywords: - bam - sam - cram - fastq tools: - samtools: description: | SAMtools is a set of utilities for interacting with and post-processing short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. These files are generated as output by short read aligners like BWA. homepage: http://www.htslib.org/ documentation: http://www.htslib.org/doc/samtools.html doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] input: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - input: type: file description: BAM/CRAM/SAM file pattern: "*.{bam,cram,sam}" - interleave: type: boolean description: Set true for interleaved fastq file output: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - versions: type: file description: File containing software versions pattern: "versions.yml" - fastq: type: file description: Compressed FASTQ file(s) with reads with either the READ1 or READ2 flag set in separate files. pattern: "*_{1,2}.fastq.gz" - interleaved: type: file description: Compressed FASTQ file with reads with either the READ1 or READ2 flag set in a combined file. Needs collated input file. pattern: "*_interleaved.fastq.gz" - singleton: type: file description: Compressed FASTQ file with singleton reads pattern: "*_singleton.fastq.gz" - other: type: file description: Compressed FASTQ file with reads with either both READ1 and READ2 flags set or unset pattern: "*_other.fastq.gz" authors: - "@priyanka-surana" - "@suzannejin"