// // Process short raw reads with AdapterRemoval // include { ADAPTERREMOVAL as ADAPTERREMOVAL_SINGLE } from '../../modules/nf-core/adapterremoval/main' include { ADAPTERREMOVAL as ADAPTERREMOVAL_PAIRED } from '../../modules/nf-core/adapterremoval/main' include { CAT_FASTQ } from '../../modules/nf-core/cat/fastq/main' workflow SHORTREAD_ADAPTERREMOVAL { take: reads // [[meta], [reads]] adapterlist // file main: ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() ch_input_for_adapterremoval = reads .branch{ single: it[0].single_end paired: !it[0].single_end } ADAPTERREMOVAL_SINGLE ( ch_input_for_adapterremoval.single, adapterlist ) ADAPTERREMOVAL_PAIRED ( ch_input_for_adapterremoval.paired, adapterlist ) /* * Due to the ~slightly~ very ugly output implementation of the current AdapterRemoval2 version, each file * has to be exported in a separate channel and we must manually recombine when necessary. */ if ( params.shortread_qc_mergepairs && params.shortread_qc_includeunmerged ) { ch_concat_fastq = Channel.empty() .mix( ADAPTERREMOVAL_PAIRED.out.collapsed, ADAPTERREMOVAL_PAIRED.out.collapsed_truncated, ADAPTERREMOVAL_PAIRED.out.singles_truncated, ADAPTERREMOVAL_PAIRED.out.paired_truncated ) .map { meta, reads -> def meta_new = meta.clone() meta_new.single_end = true [meta_new, reads] } .groupTuple() // Paired-end reads cause a nested tuple during grouping. // We want to present a flat list of files to `CAT_FASTQ`. .map { meta, fastq -> [meta, fastq.flatten()] } CAT_FASTQ(ch_concat_fastq) ch_adapterremoval_reads_prepped = CAT_FASTQ.out.reads .mix(ADAPTERREMOVAL_SINGLE.out.singles_truncated) } else if ( params.shortread_qc_mergepairs && !params.shortread_qc_includeunmerged ) { ch_concat_fastq = Channel.empty() .mix( ADAPTERREMOVAL_PAIRED.out.collapsed, ADAPTERREMOVAL_PAIRED.out.collapsed_truncated ) .map { meta, reads -> def meta_new = meta.clone() meta_new.single_end = true [meta_new, reads] } .groupTuple() .map { meta, fastq -> [meta, fastq.flatten()] } CAT_FASTQ(ch_concat_fastq) ch_adapterremoval_reads_prepped = CAT_FASTQ.out.reads .mix(ADAPTERREMOVAL_SINGLE.out.singles_truncated) } else { ch_adapterremoval_reads_prepped = ADAPTERREMOVAL_PAIRED.out.paired_truncated .mix(ADAPTERREMOVAL_SINGLE.out.singles_truncated) } ch_versions = ch_versions.mix( ADAPTERREMOVAL_SINGLE.out.versions.first() ) ch_versions = ch_versions.mix( ADAPTERREMOVAL_PAIRED.out.versions.first() ) ch_multiqc_files = ch_multiqc_files.mix( ADAPTERREMOVAL_PAIRED.out.settings, ADAPTERREMOVAL_SINGLE.out.settings ) emit: reads = ch_adapterremoval_reads_prepped // channel: [ val(meta), [ reads ] ] versions = ch_versions // channel: [ versions.yml ] mqc = ch_multiqc_files }