/* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Config file for defining DSL2 per module options and publishing paths ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Available keys to override module options: ext.args = Additional arguments appended to command in module. ext.args2 = Second set of arguments appended to command in module (multi-tool modules). ext.args3 = Third set of arguments appended to command in module (multi-tool modules). ext.prefix = File name prefix for output files. ---------------------------------------------------------------------------------------- */ process { withName: SAMPLESHEET_CHECK { publishDir = [ path: { "${params.outdir}/pipeline_info" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } withName: DATABASE_CHECK { publishDir = [ path: { "${params.outdir}/pipeline_info" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } withName: FASTQC { ext.args = '--quiet' ext.prefix = { "${meta.id}_${meta.run_accession}_raw" } publishDir = [ path: { "${params.outdir}/fastqc/raw" }, mode: params.publish_dir_mode, pattern: '*.html' ] } withName: FASTQC_PROCESSED { ext.args = '--quiet' ext.prefix = { "${meta.id}_${meta.run_accession}_processed" } publishDir = [ path: { "${params.outdir}/fastqc/processed" }, mode: params.publish_dir_mode, pattern: '*.html' ] } withName: FASTP_SINGLE { ext.args = [ // trimming options params.shortread_qc_skipadaptertrim ? "--disable_adapter_trimming" : "", params.shortread_qc_adapter1 ? "--adapter_sequence ${params.shortread_qc_adapter1}" : "", // filtering options "--length_required ${params.shortread_qc_minlength}", (params.perform_shortread_complexityfilter && params.shortread_complexityfilter_tool == 'fastp') ? "--low_complexity_filter --complexity_threshold ${params.shortread_complexityfilter_fastp_threshold}" : '' ].join(' ').trim() ext.prefix = { "${meta.id}_${meta.run_accession}" } publishDir = [ path: { "${params.outdir}/fastp" }, mode: params.publish_dir_mode, pattern: '*.fastq.gz', enabled: params.save_preprocessed_reads ] } withName: FASTP_PAIRED { ext.args = [ // collapsing options - option to retain singletons params.shortread_qc_excludeunmerged ? '' : "--include_unmerged", // trimming options params.shortread_qc_skipadaptertrim ? "--disable_adapter_trimming" : "", params.shortread_qc_adapter1 ? "--adapter_sequence ${params.shortread_qc_adapter1}" : "", params.shortread_qc_adapter2 ? "--adapter_sequence_r2 ${params.shortread_qc_adapter2}" : "--detect_adapter_for_pe", // filtering options "--length_required ${params.shortread_qc_minlength}", params.perform_shortread_complexityfilter && params.shortread_complexityfilter_tool == 'fastp' ? "--low_complexity_filter --complexity_threshold ${params.shortread_complexityfilter_fastp_threshold}" : '' ].join(' ').trim() ext.prefix = { "${meta.id}_${meta.run_accession}" } publishDir = [ path: { "${params.outdir}/fastp" }, mode: params.publish_dir_mode, pattern: '*.fastq.gz', enabled: params.save_preprocessed_reads ] } withName: ADAPTERREMOVAL_SINGLE { ext.args = [ // trimming options params.shortread_qc_skipadaptertrim ? "--adapter1 '' --adapter2 ''" : "", params.shortread_qc_adapter1 ? "--adapter1 ${params.shortread_qc_adapter1}" : "", // filtering options "--minlength ${params.shortread_qc_minlength}" ].join(' ').trim() ext.prefix = { "${meta.id}_${meta.run_accession}" } publishDir = [ path: { "${params.outdir}/adapterremoval" }, mode: params.publish_dir_mode, pattern: '*.fastq.gz', enabled: params.save_preprocessed_reads ] } withName: ADAPTERREMOVAL_PAIRED { ext.args = [ // collapsing options params.shortread_qc_mergepairs ? "--collapse" : "", // trimming options params.shortread_qc_skipadaptertrim ? "--adapter1 '' --adapter2 ''" : "", params.shortread_qc_adapter1 ? "--adapter1 ${params.shortread_qc_adapter1}" : "", params.shortread_qc_adapter2 ? "--adapter2 ${params.shortread_qc_adapter2}" : "", // filtering options "--minlength ${params.shortread_qc_minlength}" ].join(' ').trim() ext.prefix = { "${meta.id}_${meta.run_accession}" } publishDir = [ path: { "${params.outdir}/adapterremoval" }, mode: params.publish_dir_mode, pattern: '*.fastq.gz', enabled: params.save_preprocessed_reads ] } withName: PORECHOP { ext.prefix = { "${meta.id}_${meta.run_accession}" } publishDir = [ path: { "${params.outdir}/porechop" }, mode: params.publish_dir_mode, pattern: '*.fastq.gz', enabled: params.save_preprocessed_reads ] } withName: FILTLONG { ext.args = [ "--min_length ${params.longread_qc_minlength}", "--keep_percent ${params.longread_qc_keep_percent}", "--target_bases ${params.longread_qc_target_bases}" ] .join(' ').trim() ext.prefix = { "${meta.id}_${meta.run_accession}_filtered" } publishDir = [ path: { "${params.outdir}/filtlong" }, mode: params.publish_dir_mode, pattern: '*.fastq.gz', enabled: params.save_preprocessed_reads ] } withName: BOWTIE2_BUILD { publishDir = [ path: { "${params.outdir}/bowtie2/build" }, mode: params.publish_dir_mode, enabled: params.save_hostremoval_index, pattern: 'bowtie2' ] } withName: BOWTIE2_ALIGN { ext.prefix = { "${meta.id}_${meta.run_accession}" } publishDir = [ [ path: { "${params.outdir}/bowtie2/align" }, mode: params.publish_dir_mode, pattern: '*.log' ], [ path: { "${params.outdir}/bowtie2/align" }, mode: params.publish_dir_mode, enabled: params.save_hostremoval_mapped, pattern: '*.bam' ], [ path: { "${params.outdir}/bowtie2/align" }, mode: params.publish_dir_mode, enabled: params.save_hostremoval_unmapped, pattern: '*.fastq.gz' ] ] } withName: MINIMAP2_INDEX { ext.args = '-x map-ont' publishDir = [ path: { "${params.outdir}/minimap2/index" }, mode: params.publish_dir_mode, enabled: params.save_hostremoval_index, pattern: 'minimap2' ] } withName: MINIMAP2_ALIGN { ext.prefix = { "${meta.id}_${meta.run_accession}" } publishDir = [ path: { "${params.outdir}/minimap2/align" }, mode: params.publish_dir_mode, enabled: params.save_hostremoval_mapped, pattern: '*.bam' ] } withName: SAMTOOLS_VIEW { ext.args = '-f 4' ext.prefix = { "${meta.id}.mapped.sorted" } publishDir = [ path: { "${params.outdir}/samtools/view" }, mode: params.publish_dir_mode, enabled: params.save_hostremoval_unmapped, pattern: '*.bam' ] } withName: SAMTOOLS_BAM2FQ { ext.prefix = { "${meta.id}_${meta.run_accession}" } publishDir = [ path: { "${params.outdir}/samtools/bam2fq" }, mode: params.publish_dir_mode, enabled: params.save_hostremoval_unmapped, pattern: '*.fq.gz' ] } withName: BBMAP_BBDUK { ext.args = [ "entropy=${params.shortread_complexityfilter_entropy}", "entropywindow=${params.shortread_complexityfilter_bbduk_windowsize}", params.shortread_complexityfilter_bbduk_mask ? "entropymask=t" : "entropymask=f" ].join(' ').trim() ext.prefix = { "${meta.id}-${meta.run_accession}" } publishDir = [ path: { "${params.outdir}/bbduk/" }, mode: params.publish_dir_mode, pattern: '*.{fastq.gz,log}', enabled: params.save_complexityfiltered_reads ] } withName: PRINSEQPLUSPLUS { ext.args = [ params.shortread_complexityfilter_prinseqplusplus_mode == 'dust' ? "-lc_dust=${params.shortread_complexityfilter_prinseqplusplus_dustscore}" : "-lc_entropy=${params.shortread_complexityfilter_entropy}", "-trim_qual_left=0 -trim_qual_left=0 -trim_qual_window=0 -trim_qual_step=0" ].join(' ').trim() ext.prefix = { "${meta.id}-${meta.run_accession}" } publishDir = [ path: { "${params.outdir}/prinseqplusplus/" }, mode: params.publish_dir_mode, pattern: '*{_good_out.fastq.gz,_good_out_R1.fastq.gz,_good_out_R2.fastq.gz,log}', enabled: params.save_complexityfiltered_reads ] } withName: CAT_FASTQ { ext.prefix = { "${meta.id}" } publishDir = [ path: { "${params.outdir}/run_merging/" }, mode: params.publish_dir_mode, pattern: '*.fastq.gz', enabled: params.save_runmerged_reads ] } withName: MALT_RUN { ext.args = { "${meta.db_params}" } // one run with multiple samples, so fix ID to just db name to ensure clean log name ext.prefix = { "${meta.db_name}" } publishDir = [ path: { "${params.outdir}/malt/${meta.db_name}" }, mode: params.publish_dir_mode, pattern: '*.{rma6,log,sam}' ] } withName: MEGAN_RMA2INFO { ext.args = "-c2c Taxonomy" ext.prefix = { "${meta.id}" } publishDir = [ path: { "${params.outdir}/malt/${meta.db_name}" }, mode: params.publish_dir_mode, pattern: '*.{txt.gz,megan}' ] } withName: KRAKEN2_KRAKEN2 { ext.args = { "${meta.db_params}" } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ path: { "${params.outdir}/kraken2/${meta.db_name}" }, mode: params.publish_dir_mode, pattern: '*.{txt,report,fastq.gz}' ] } withName: METAPHLAN3 { ext.args = { "${meta.db_params}" } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ path: { "${params.outdir}/metaphlan3/${meta.db_name}" }, mode: params.publish_dir_mode, pattern: '*.{biom,txt}' ] } withName: CENTRIFUGE_CENTRIFUGE { publishDir = [ path: { "${params.outdir}/centrifuge/${meta.db_name}" }, mode: params.publish_dir_mode, pattern: '*.{txt,sam,gz}' ] ext.args = { "${meta.db_params}" } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } } withName: CENTRIFUGE_KREPORT { ext.args = { "${meta.db_params}" } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ path: { "${params.outdir}/centrifuge/${meta.db_name}" }, mode: params.publish_dir_mode, pattern: '*.{txt}' ] } withName: KAIJU_KAIJU { publishDir = [ path: { "${params.outdir}/kaiju/${meta.db_name}" }, mode: params.publish_dir_mode, pattern: '*.tsv' ] ext.args = { "${meta.db_params}" } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } } withName: KAIJU_KAIJU2TABLE { ext.args = { "${meta.db_params}" } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ path: { "${params.outdir}/kaiju/${meta.db_name}" }, mode: params.publish_dir_mode, pattern: '*.{txt}' ] } withName: DIAMOND_BLASTX { ext.args = { "${meta.db_params}" } ext.prefix = params.perform_runmerging ? { "${meta.id}-${meta.db_name}" } : { "${meta.id}-${meta.run_accession}-${meta.db_name}" } publishDir = [ path: { "${params.outdir}/diamond/${meta.db_name}" }, mode: params.publish_dir_mode, pattern: '*.{blast,xml,txt,daa,sam,tsv,paf}' ] } withName: CUSTOM_DUMPSOFTWAREVERSIONS { publishDir = [ path: { "${params.outdir}/pipeline_info" }, mode: params.publish_dir_mode, pattern: '*_versions.yml' ] } withName: MULTIQC { publishDir = [ path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } }