/* Process short raw reads with AdapterRemoval */ include { ADAPTERREMOVAL as ADAPTERREMOVAL_SINGLE } from '../../modules/nf-core/modules/adapterremoval/main' include { ADAPTERREMOVAL as ADAPTERREMOVAL_PAIRED } from '../../modules/nf-core/modules/adapterremoval/main' include { CAT_FASTQ } from '../../modules/nf-core/modules/cat/fastq/main' workflow SHORTREAD_ADAPTERREMOVAL { take: reads // [[meta], [reads]] main: ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() ch_input_for_adapterremoval = reads .branch{ single: it[0].single_end paired: !it[0].single_end } ADAPTERREMOVAL_SINGLE ( ch_input_for_adapterremoval.single, [] ) ADAPTERREMOVAL_PAIRED ( ch_input_for_adapterremoval.paired, [] ) /* * Due to the ~slightly~ very ugly output implementation of the current AdapterRemoval2 version, each file * has to be exported in a separate channel and we must manually recombine when necessary. */ if ( params.shortread_clipmerge_mergepairs && !params.shortread_clipmerge_excludeunmerged ) { ch_adapterremoval_for_cat = ADAPTERREMOVAL_PAIRED.out.collapsed .mix( ADAPTERREMOVAL_PAIRED.out.collapsed_truncated, ADAPTERREMOVAL_PAIRED.out.singles_truncated, ADAPTERREMOVAL_PAIRED.out.pair1_truncated, ADAPTERREMOVAL_PAIRED.out.pair2_truncated ) .map { meta, reads -> def meta_new = meta.clone() meta_new.single_end = true [ meta_new, reads ] } .groupTuple() ch_adapterremoval_reads_prepped = CAT_FASTQ ( ch_adapterremoval_for_cat ).reads .mix( ADAPTERREMOVAL_SINGLE.out.singles_truncated ) } else if ( params.shortread_clipmerge_mergepairs && params.shortread_clipmerge_excludeunmerged ) { ch_adapterremoval_for_cat = ADAPTERREMOVAL_PAIRED.out.collapsed .mix( ADAPTERREMOVAL_PAIRED.out.collapsed_truncated ) .map { meta, reads -> def meta_new = meta.clone() meta_new['single_end'] = true [ meta_new, reads ] } .groupTuple(by: 0) ch_adapterremoval_reads_prepped = CAT_FASTQ ( ch_adapterremoval_for_cat ).reads .mix( ADAPTERREMOVAL_SINGLE.out.singles_truncated ) } else { ch_adapterremoval_reads_prepped = ADAPTERREMOVAL_PAIRED.out.pair1_truncated .join( ADAPTERREMOVAL_PAIRED.out.pair2_truncated ) .groupTuple() .map { meta, pair1, pair2 -> [ meta, [ pair1, pair2 ].flatten() ] } .mix( ADAPTERREMOVAL_SINGLE.out.singles_truncated ) } ch_processed_reads = ch_adapterremoval_reads_prepped ch_versions = ch_versions.mix( ADAPTERREMOVAL_SINGLE.out.versions.first() ) ch_versions = ch_versions.mix( ADAPTERREMOVAL_PAIRED.out.versions.first() ) ch_multiqc_files = ch_multiqc_files.mix( ADAPTERREMOVAL_PAIRED.out.log.collect{it[1]}, ADAPTERREMOVAL_SINGLE.out.log.collect{it[1]} ) emit: reads = ch_processed_reads // channel: [ val(meta), [ reads ] ] versions = ch_versions // channel: [ versions.yml ] mqc = ch_multiqc_files }