// // Perform read trimming and merging // include { SHORTREAD_FASTP } from './shortread_fastp' include { SHORTREAD_ADAPTERREMOVAL } from './shortread_adapterremoval' include { FASTQC as FASTQC_PROCESSED } from '../../modules/nf-core/fastqc/main' include { FALCO as FALCO_PROCESSED } from '../../modules/nf-core/falco/main' workflow SHORTREAD_PREPROCESSING { take: reads // [ [ meta ], [ reads ] ] adapterlist // file main: ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() if ( params.shortread_qc_tool == "fastp" ) { ch_processed_reads = SHORTREAD_FASTP ( reads ).reads ch_versions = ch_versions.mix( SHORTREAD_FASTP.out.versions ) ch_multiqc_files = ch_multiqc_files.mix( SHORTREAD_FASTP.out.mqc ) } else if ( params.shortread_qc_tool == "adapterremoval" ) { ch_processed_reads = SHORTREAD_ADAPTERREMOVAL ( reads ).reads ch_versions = ch_versions.mix( SHORTREAD_ADAPTERREMOVAL.out.versions ) ch_multiqc_files = ch_multiqc_files.mix( SHORTREAD_ADAPTERREMOVAL.out.mqc ) } else { ch_processed_reads = reads } if (params.preprocessing_qc_tool == 'fastqc') { FASTQC_PROCESSED ( ch_processed_reads ) ch_versions = ch_versions.mix( FASTQC_PROCESSED.out.versions ) ch_multiqc_files = ch_multiqc_files.mix( FASTQC_PROCESSED.out.zip ) } else if (params.preprocessing_qc_tool == 'falco') { FALCO_PROCESSED ( ch_processed_reads ) ch_versions = ch_versions.mix( FALCO_PROCESSED.out.versions ) ch_multiqc_files = ch_multiqc_files.mix( FALCO_PROCESSED.out.txt ) } emit: reads = ch_processed_reads // channel: [ val(meta), [ reads ] ] versions = ch_versions // channel: [ versions.yml ] mqc = ch_multiqc_files }