// // Check input samplesheet and get read channels // include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' workflow INPUT_CHECK { take: samplesheet // file: /path/to/samplesheet.csv main: parsed_samplesheet = SAMPLESHEET_CHECK ( samplesheet ) .csv .splitCsv ( header:true, sep:',' ) .branch { row -> fasta: row.fasta != '' nanopore: row.instrument_platform == 'OXFORD_NANOPORE' fastq: true } fastq = parsed_samplesheet.fastq .map { create_fastq_channel(it) } nanopore = parsed_samplesheet.nanopore .map { create_fastq_channel(it) } fasta = parsed_samplesheet.fasta .map { create_fasta_channel(it) } emit: fastq = fastq ?: [] // channel: [ val(meta), [ reads ] ] nanopore = nanopore ?: [] // channel: [ val(meta), [ reads ] ] fasta = fasta ?: [] // channel: [ val(meta), fasta ] versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] } // Function to get list of [ meta, [ fastq_1, fastq_2 ] ] def create_fastq_channel(LinkedHashMap row) { // create meta map def meta = row.subMap(['sample', 'run_accession', 'instrument_platform']) meta.id = meta.sample meta.single_end = row.single_end.toBoolean() meta.is_fasta = false // add path(s) of the fastq file(s) to the meta map if (!file(row.fastq_1).exists()) { error("ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}") } if (meta.single_end) { return [ meta, [ file(row.fastq_1) ] ] } else { if (meta.instrument_platform == 'OXFORD_NANOPORE') { if (row.fastq_2 != '') { error("ERROR: Please check input samplesheet -> For Oxford Nanopore reads Read 2 FastQ should be empty!\n${row.fastq_2}") } return [ meta, [ file(row.fastq_1) ] ] } else { if (!file(row.fastq_2).exists()) { error("ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}") } return [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] } } } // Function to get list of [ meta, fasta ] def create_fasta_channel(LinkedHashMap row) { def meta = row.subMap(['sample', 'run_accession', 'instrument_platform']) meta.id = meta.sample meta.single_end = true meta.is_fasta = true if (!file(row.fasta).exists()) { error("ERROR: Please check input samplesheet -> FastA file does not exist!\n${row.fasta}") } return [ meta, [ file(row.fasta) ] ] }