// // Remove host reads via alignment and export off-target reads // include { BOWTIE2_BUILD } from '../../modules/nf-core/bowtie2/build/main' include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/main' workflow SHORTREAD_HOSTREMOVAL { take: reads // [ [ meta ], [ reads ] ] reference // /path/to/fasta index // /path/to/index main: ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() if ( !params.shortread_hostremoval_index ) { ch_bowtie2_index = BOWTIE2_BUILD ( reference ).index ch_versions = ch_versions.mix( BOWTIE2_BUILD.out.versions ) } else { ch_bowtie2_index = index.first() } BOWTIE2_ALIGN ( reads, ch_bowtie2_index, true, false ) ch_versions = ch_versions.mix( BOWTIE2_ALIGN.out.versions.first() ) ch_multiqc_files = ch_multiqc_files.mix( BOWTIE2_ALIGN.out.log ) emit: reads = BOWTIE2_ALIGN.out.fastq // channel: [ val(meta), [ reads ] ] versions = ch_versions // channel: [ versions.yml ] mqc = ch_multiqc_files }