// // Remove host reads via alignment and export off-target reads // include { MINIMAP2_INDEX } from '../../modules/nf-core/minimap2/index/main' include { MINIMAP2_ALIGN } from '../../modules/nf-core/minimap2/align/main' include { SAMTOOLS_VIEW } from '../../modules/nf-core/samtools/view/main' include { SAMTOOLS_BAM2FQ } from '../../modules/nf-core/samtools/bam2fq/main' workflow LONGREAD_HOSTREMOVAL { take: reads // [ [ meta ], [ reads ] ] reference // /path/to/fasta index // /path/to/index main: ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() if ( !params.longread_hostremoval_index ) { ch_minimap2_index = MINIMAP2_INDEX ( reference ).index ch_versions = ch_versions.mix( MINIMAP2_INDEX.out.versions ) } else { ch_minimap2_index = index } MINIMAP2_ALIGN ( reads, ch_minimap2_index, true, false, false ) ch_versions = ch_versions.mix( MINIMAP2_ALIGN.out.versions.first() ) ch_minimap2_mapped = MINIMAP2_ALIGN.out.bam .map { meta, reads -> [ meta, reads, [] ] } SAMTOOLS_VIEW ( ch_minimap2_mapped , [], [] ) ch_versions = ch_versions.mix( SAMTOOLS_VIEW.out.versions.first() ) SAMTOOLS_BAM2FQ ( SAMTOOLS_VIEW.out.bam, false ) ch_versions = ch_versions.mix( SAMTOOLS_BAM2FQ.out.versions.first() ) emit: reads = SAMTOOLS_BAM2FQ.out.reads // channel: [ val(meta), [ reads ] ] versions = ch_versions // channel: [ versions.yml ] }