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taxprofiler/assets/multiqc_config.yml
Sofia Stamouli 07a1cfae62
Update assets/multiqc_config.yml
Co-authored-by: James A. Fellows Yates <jfy133@gmail.com>
2022-12-16 11:46:42 +01:00

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YAML

report_comment: >
This report has been generated by the <a href="https://github.com/nf-core/taxprofiler" target="_blank">nf-core/taxprofiler</a>
analysis pipeline. For information about how to interpret these results, please see the
<a href="https://nf-co.re/taxprofiler" target="_blank">documentation</a>.
report_section_order:
"nf-core-taxprofiler-methods-description":
order: -1000
software_versions:
order: -1001
"nf-core-taxprofiler-summary":
order: -1002
export_plots: true
custom_logo: "nf-core-taxprofiler_logo_custom_light.png"
custom_logo_url: https://nf-co.re/taxprofiler
custom_logo_title: "nf-core/taxprofiler"
run_modules:
- fastqc
- adapterRemoval
- bbduk
- prinseqplusplus
- fastp
- filtlong
- bowtie2
- minimap2
- samtools
- kraken
- kaiju
- metaphlan
- diamond
- malt
- motus
- porechop
- custom_content
#extra_fn_clean_exts:
# - '_fastp'
# - '.pe.settings'
# - '.se.settings'
top_modules:
- "fastqc":
name: "FastQC (pre-Trimming)"
path_filters:
- "*raw_*fastqc.zip"
- "fastqc":
name: "Falco (pre-Trimming)"
path_filters:
- "*_raw_falco_*_report.html"
- "fastp"
- "adapterRemoval"
- "porechop"
- "fastqc":
name: "FastQC (post-Trimming)"
path_filters:
- "*_processed_*fastqc.zip"
- "fastqc":
name: "Falco (post-Trimming)"
path_filters:
- "*_processed_falco_*_report.html"
- "bbduk"
- "prinseqplusplus"
- "filtlong"
- "bowtie2":
name: "bowtie2"
- "samtools":
name: "Samtools Stats"
- "kraken":
name: "Kraken"
path_filters:
- "*.kraken2.kraken2.report.txt"
- "kraken":
name: "Bracken"
anchor: "bracken"
target: "Bracken"
doi: "10.7717/peerj-cs.104"
info: "Estimates species abundances in metagenomics samples by probabilistically re-distributing reads in the taxonomic tree."
extra: "Note: plot title will say Kraken2 due to the first step of bracken producing the same output format as Kraken. Abundance information is currently not supported in MultiQC."
path_filters:
- "*.bracken.kraken2.report.txt"
- "kraken":
name: "Centrifuge"
anchor: "centrifuge"
target: "Centrifuge"
doi: "10.1101/gr.210641.116"
info: "is a very rapid and memory-efficient system for the classification of DNA sequences from microbial samples. The system uses a novel indexing scheme based on the Burrows-Wheeler transform (BWT) and the Ferragina-Manzini (FM) index. Note: Figure title"
extra: "Note: plot title will say Kraken2 due to Centrifuge producing the same output format as Kraken. If activated, see the actual Kraken2 results in the section above."
path_filters:
- "*.centrifuge.txt"
- "malt":
name: "MALT"
- "diamond"
- "kaiju":
name: "Kaiju"
- "motus"
#It is not possible to set placement for custom kraken and centrifuge columns.
table_columns_placement:
FastQC (pre-Trimming):
total_sequences: 100
avg_sequence_length: 110
percent_duplicates: 120
percent_gc: 130
percent_fails: 140
Falco (pre-Trimming):
total_sequences: 200
avg_sequence_length: 210
percent_duplicates: 220
percent_gc: 230
percent_fails: 240
fastp:
pct_adapter: 300
pct_surviving: 310
pct_duplication: 320
after_filtering_gc_content: 330
after_filtering_q30_rate: 340
after_filtering_q30_bases: 350
Adapter Removal:
aligned_total: 360
percent_aligned: 370
percent_collapsed: 380
percent_discarded: 390
FastQC (post-Trimming):
total_sequences: 400
avg_sequence_length: 410
percent_duplicates: 420
percent_gc: 430
percent_fails: 440
Falco (post-Trimming):
total_sequences: 500
avg_sequence_length: 510
percent_duplicates: 520
percent_gc: 530
percent_fails: 540
bowtie2:
overall_alignment_rate: 600
Samtools Stats:
raw_total_sequences: 700
reads_mapped: 710
reads_mapped_percent: 720
reads_properly_paired_percent: 730
non-primary_alignments: 740
reads_MQ0_percent: 750
error_rate: 760
MALT:
Num. of queries: 1000
Total reads: 1100
Mappability: 1200
Assig. Taxonomy: 1300
Taxonomic assignment success: 1400
Kaiju:
assigned: 2000
"% Assigned": 2100
"% Unclassified": 2200
table_columns_visible:
FastQC (pre-Trimming):
total_sequences: True
avg_sequence_length: True
percent_duplicates: True
percent_gc: True
percent_fails: False
Falco (pre-Trimming):
total_sequences: True
avg_sequence_length: True
percent_duplicates: True
percent_gc: True
percent_fails: False
fastp:
pct_adapter: True
pct_surviving: True
pct_duplication: False
after_filtering_gc_content: False
after_filtering_q30_rate: False
after_filtering_q30_bases: False
Adapter Removal:
aligned_total: True
percent_aligned: True
percent_collapsed: True
percent_discarded: False
FastQC (post-Trimming):
total_sequences: True
avg_sequence_length: True
percent_duplicates: False
percent_gc: False
percent_fails: False
Falco (post-Trimming):
total_sequences: True
avg_sequence_length: True
percent_duplicates: False
percent_gc: False
percent_fails: False
bowtie2:
overall_alignment_rate: True
Samtools Stats:
raw_total_sequences: True
reads_mapped: True
reads_mapped_percent: True
reads_properly_paired_percent: False
non-primary_alignments: False
reads_MQ0_percent: False
error_rate: False
Kraken:
"% Unclassified": True
"% Top 5": False
Bracken:
"% Unclassified": True
"% Top 5": False
Centrifuge:
"% Unclassified": True
"% Top 5": False
MALT:
Num. of queries: True
Total reads: True
Mappability: True
Assig. Taxonomy: False
Taxonomic assignment success: True
Kaiju:
assigned: False
"% Assigned": False
"% Unclassified": True
table_columns_name:
FastQC (pre-Trimming):
total_sequences: "Nr. Input Reads"
avg_sequence_length: "Length Input Reads"
percent_gc: "% GC Input Reads"
percent_duplicates: "% Dups Input Reads"
percent_fails: "% Failed Input Reads"
Falco (pre-Trimming):
total_sequences: "Nr. Input Reads"
avg_sequence_length: "Length Input Reads"
percent_gc: "% GC Input Reads"
percent_duplicates: "% Dups Input Reads"
percent_fails: "% Failed Input Reads"
FastQC (post-Trimming):
total_sequences: "Nr. Processed Reads"
avg_sequence_length: "Length Processed Reads"
percent_gc: "% GC Processed Reads"
percent_duplicates: "% Dups Processed Reads"
percent_fails: "% Failed Processed Reads"
Falco (post-Trimming):
total_sequences: "Nr. Processed Reads"
avg_sequence_length: "Length Processed Reads"
percent_gc: "% GC Processed Reads"
percent_duplicates: "% Dups Processed Reads"
percent_fails: "% Failed Processed Reads"
Samtools Stats:
raw_total_sequences: "Nr. Reads Into Mapping"
reads_mapped: "Nr. Mapped Reads"
reads_mapped_percent: "% Mapped Reads"
extra_fn_clean_exts:
- ".kraken2.kraken2.report.txt"
- ".centrifuge.txt"
- ".bracken.kraken2.report.txt"
- ".settings"