mirror of
https://github.com/MillironX/taxprofiler.git
synced 2024-11-29 16:29:55 +00:00
61 lines
2.3 KiB
Text
Generated
61 lines
2.3 KiB
Text
Generated
process CENTRIFUGE_CENTRIFUGE {
|
|
tag "$meta.id"
|
|
label 'process_high'
|
|
|
|
conda (params.enable_conda ? "bioconda::centrifuge=1.0.4_beta" : null)
|
|
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
|
'https://depot.galaxyproject.org/singularity/centrifuge:1.0.4_beta--h9a82719_6' :
|
|
'quay.io/biocontainers/centrifuge:1.0.4_beta--h9a82719_6' }"
|
|
|
|
input:
|
|
tuple val(meta), path(reads)
|
|
path db
|
|
val save_unaligned
|
|
val save_aligned
|
|
val sam_format
|
|
|
|
output:
|
|
tuple val(meta), path('*report.txt') , emit: report
|
|
tuple val(meta), path('*results.txt') , emit: results
|
|
tuple val(meta), path('*.sam') , optional: true, emit: sam
|
|
tuple val(meta), path('*.mapped.fastq{,.1,.2}.gz') , optional: true, emit: fastq_mapped
|
|
tuple val(meta), path('*.unmapped.fastq{,.1,.2}.gz') , optional: true, emit: fastq_unmapped
|
|
path "versions.yml" , emit: versions
|
|
|
|
when:
|
|
task.ext.when == null || task.ext.when
|
|
|
|
script:
|
|
def args = task.ext.args ?: ''
|
|
def prefix = task.ext.prefix ?: "${meta.id}"
|
|
def paired = meta.single_end ? "-U ${reads}" : "-1 ${reads[0]} -2 ${reads[1]}"
|
|
def unaligned = ''
|
|
def aligned = ''
|
|
if (meta.single_end) {
|
|
unaligned = save_unaligned ? "--un-gz ${prefix}.unmapped.fastq.gz" : ''
|
|
aligned = save_aligned ? "--al-gz ${prefix}.mapped.fastq.gz" : ''
|
|
} else {
|
|
unaligned = save_unaligned ? "--un-conc-gz ${prefix}.unmapped.fastq.gz" : ''
|
|
aligned = save_aligned ? "--al-conc-gz ${prefix}.mapped.fastq.gz" : ''
|
|
}
|
|
def sam_output = sam_format ? "--out-fmt 'sam'" : ''
|
|
"""
|
|
## we add "-no-name ._" to ensure silly Mac OSX metafiles files aren't included
|
|
db_name=`find -L ${db} -name "*.1.cf" -not -name "._*" | sed 's/.1.cf//'`
|
|
centrifuge \\
|
|
-x \$db_name \\
|
|
-p $task.cpus \\
|
|
$paired \\
|
|
--report-file ${prefix}.report.txt \\
|
|
-S ${prefix}.results.txt \\
|
|
$unaligned \\
|
|
$aligned \\
|
|
$sam_output \\
|
|
$args
|
|
|
|
cat <<-END_VERSIONS > versions.yml
|
|
"${task.process}":
|
|
centrifuge: \$( centrifuge --version | sed -n 1p | sed 's/^.*centrifuge-class version //')
|
|
END_VERSIONS
|
|
"""
|
|
}
|