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taxprofiler/subworkflows/local/shortread_preprocessing.nf
2022-03-25 14:58:06 +01:00

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//
// Check input samplesheet and get read channels
//
include { SHORTREAD_FASTP } from './shortread_fastp'
include { FASTQC as FASTQC_PROCESSED } from '../../modules/nf-core/modules/fastqc/main'
workflow SHORTREAD_PREPROCESSING {
take:
reads // file: /path/to/samplesheet.csv
main:
ch_versions = Channel.empty()
ch_multiqc_files = Channel.empty()
//
// STEP: Read clipping and merging
//
// TODO give option to clip only and retain pairs
// TODO give option to retain singletons (probably fastp option likely)
// TODO move to subworkflow
if ( params.shortread_clipmerge_tool == "fastp" ) {
ch_processed_reads = SHORTREAD_FASTP ( reads ).reads
ch_versions = ch_versions.mix( SHORTREAD_FASTP.out.versions )
ch_multiqc_files = ch_multiqc_files.mix( SHORTREAD_FASTP.out.mqc )
} else {
ch_processed_reads = reads
}
//FASTQC_PROCESSED ( ch_processed_reads )
//ch_versions = ch_versions.mix( FASTQC_PROCESSED.out.versions )
//ch_multiqc_files = ch_multiqc_files.mix( FASTQC_PROCESSED.out.zip.collect{it[1]} )
emit:
// TODO: problem, this is being exported as a multi-channel output? This is why FASTQC is broken
reads = ch_processed_reads // channel: [ val(meta), [ reads ] ]
versions = ch_versions // channel: [ versions.yml ]
mqc = ch_multiqc_files
}