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49 lines
1.9 KiB
Plaintext
49 lines
1.9 KiB
Plaintext
process METAPHLAN3_METAPHLAN3 {
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tag "$meta.id"
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label 'process_high'
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conda "bioconda::metaphlan=3.0.12"
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/metaphlan:3.0.12--pyhb7b1952_0' :
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'quay.io/biocontainers/metaphlan:3.0.12--pyhb7b1952_0' }"
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input:
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tuple val(meta), path(input)
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path metaphlan_db
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output:
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tuple val(meta), path("*_profile.txt") , emit: profile
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tuple val(meta), path("*.biom") , emit: biom
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tuple val(meta), path('*.bowtie2out.txt'), optional:true, emit: bt2out
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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def prefix = task.ext.prefix ?: "${meta.id}"
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def input_type = ("$input".endsWith(".fastq.gz") || "$input".endsWith(".fq.gz")) ? "--input_type fastq" : ("$input".contains(".fasta")) ? "--input_type fasta" : ("$input".endsWith(".bowtie2out.txt")) ? "--input_type bowtie2out" : "--input_type sam"
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def input_data = ("$input_type".contains("fastq")) && !meta.single_end ? "${input[0]},${input[1]}" : "$input"
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def bowtie2_out = "$input_type" == "--input_type bowtie2out" || "$input_type" == "--input_type sam" ? '' : "--bowtie2out ${prefix}.bowtie2out.txt"
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"""
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BT2_DB=`find -L "${metaphlan_db}" -name "*rev.1.bt2" -exec dirname {} \\;`
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metaphlan \\
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--nproc $task.cpus \\
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$input_type \\
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$input_data \\
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$args \\
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$bowtie2_out \\
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--bowtie2db \$BT2_DB \\
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--biom ${prefix}.biom \\
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--output_file ${prefix}_profile.txt
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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metaphlan3: \$(metaphlan --version 2>&1 | awk '{print \$3}')
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END_VERSIONS
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"""
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}
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