haplotyper-battle-royale/main.nf

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Executable file

#!/usr/bin/env nextflow
include { validateParameters; fromSamplesheet } from 'plugin/nf-validation'
include { CLIQUESNV } from './modules/cliquesnv'
include { EFETCH } from './modules/efetch'
include { FASTERQ_DUMP } from './modules/fasterq_dump'
include { HAPLINK_HAPLOTYPES as HAPLINK_ML_HAPLOTYPES } from './modules/haplink/haplotypes'
include { HAPLINK_HAPLOTYPES as HAPLINK_RAW_HAPLOTYPES } from './modules/haplink/haplotypes'
include { HAPLINK_SEQUENCES } from './modules/haplink/sequences'
include { HAPLINK_VARIANTS } from './modules/haplink/variants'
include { MINIMAP2 } from './modules/minimap2'
include { NANOFILT } from './modules/nanofilt'
include { QUASIRECOMB } from './modules/quasirecomb'
include { SHORAH_AMPLICON } from './modules/shorah/amplicon'
include { SHORAH_SHOTGUN } from './modules/shorah/shotgun'
include { VIQUAS } from './modules/viquas'
workflow {
validateParameters()
Channel
.fromSamplesheet("input")
.set { ch_sras }
FASTERQ_DUMP( ch_sras )
FASTERQ_DUMP
.out
.set { ch_input }
EFETCH("${params.reference}")
EFETCH
.out
.set { ch_reference }
NANOFILT( ch_input )
NANOFILT
.out
.set { ch_reads }
MINIMAP2( ch_reads, ch_reference )
MINIMAP2
.out
.set { ch_alignments }
CLIQUESNV(
ch_alignments,
'snv-pacbio'
)
HAPLINK_VARIANTS( ch_alignments, ch_reference )
HAPLINK_VARIANTS
.out
.set { ch_variants }
ch_alignments
.join( ch_variants )
.set { ch_haplotype_calling }
HAPLINK_RAW_HAPLOTYPES(
ch_haplotype_calling,
ch_reference
)
HAPLINK_RAW_HAPLOTYPES
.out
.map{ [ it[0], 'HAPLINK_RAW_HAPLOTYPES', it[1] ] }
.set{ ch_raw_haplotypes }
HAPLINK_ML_HAPLOTYPES(
ch_haplotype_calling,
ch_reference
)
HAPLINK_ML_HAPLOTYPES
.out
.map{ [ it[0], 'HAPLINK_ML_HAPLOTYPES', it[1] ] }
.set{ ch_ml_haplotypes }
ch_raw_haplotypes
.mix(ch_ml_haplotypes)
.set{ ch_all_haplotypes }
HAPLINK_SEQUENCES(
ch_all_haplotypes,
ch_reference
)
QUASIRECOMB( ch_alignments )
SHORAH_AMPLICON(
ch_alignments,
ch_reference
)
SHORAH_SHOTGUN(
ch_alignments,
ch_reference
)
VIQUAS(
ch_alignments,
ch_reference
)
}