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50 lines
2.5 KiB
Markdown
50 lines
2.5 KiB
Markdown
# nf-core/configs: MUNIN rnavar specific configuration
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Extra specific configuration for rnavar pipeline
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## Usage
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To use, run the pipeline with `-profile munin`.
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This will download and launch the rnavar specific [`munin.config`](../../../conf/pipeline/rnavar/munin.config) which has been pre-configured with a setup suitable for the `MUNIN` cluster.
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Example: `nextflow run nf-core/rnavar -profile munin`
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## rnavar specific configurations for MUNIN
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Specific configurations for `MUNIN` has been made for rnavar.
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Genome references
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- Path to `fasta`: `/data1/references/CTAT_GenomeLib_v37_Mar012021/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir/ref_genome.fa`
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- Path to `fasta_fai`: `/data1/references/CTAT_GenomeLib_v37_Mar012021/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir/ref_genome.fa.fai`
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- Path to `gtf`: `/data1/references/CTAT_GenomeLib_v37_Mar012021/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir/ref_annot.gtf`
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- Path to `gene_bed`: `/data1/references/CTAT_GenomeLib_v37_Mar012021/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir/ref_annot.bed`
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Known genome resources
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- Path to `dbsnp`: `/data1/references/annotations/GATK_bundle/dbsnp_146.hg38.vcf.gz`
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- Path to `dbsnp_tbi`: `/data1/references/annotations/GATK_bundle/dbsnp_146.hg38.vcf.gz.tbi`
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- Path to `known_indels`: `/data1/references/annotations/GATK_bundle/Mills_and_1000G_gold_standard.indels.hg38.vcf.gz`
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- Path to `known_indels_tbi`: `/data1/references/annotations/GATK_bundle/Mills_and_1000G_gold_standard.indels.hg38.vcf.gz.tbi`
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STAR index
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- Path to `star_index`: `/data1/references/CTAT_GenomeLib_v37_Mar012021/GRCh38_gencode_v37_CTAT_lib_Mar012021.plug-n-play/ctat_genome_lib_build_dir/STAR.2.7.9a_2x151bp/`
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- Params `read_length` set to `151`
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Variant annotation configurations
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- Params `annotation_cache` and `cadd_cache` set to `true`
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- Params `snpeff_db` set to `GRCh38.99`
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- Params `vep_cache_version` set to `99`
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- Params `vep_genome` set to `GRCh38`
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- Path to `snpeff_cache`: `/data1/cache/snpEff/`
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- Path to `vep_cache`: `/data1/cache/VEP/`
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- Path to `pon`: `/data1/PON/vcfs/BTB.PON.vcf.gz`
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- Path to `pon_index`: `/data1/PON/vcfs/BTB.PON.vcf.gz.tbi`
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- Path to `cadd_indels`: `/data1/cache/CADD/v1.4/InDels.tsv.gz`
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- Path to `cadd_indels_tbi`: `/data1/cache/CADD/v1.4/InDels.tsv.gz.tbi`
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- Path to `cadd_wg_snvs`: `/data1/cache/CADD/v1.4/whole_genome_SNVs.tsv.gz`
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- Path to `cadd_wg_snvs_tbi`: `/data1/cache/CADD/v1.4/whole_genome_SNVs.tsv.gz.tbi`
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