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275 lines
11 KiB
Markdown
275 lines
11 KiB
Markdown
# nf-core/configs: UPPMAX Configuration
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All nf-core pipelines have been successfully configured for use on the Swedish UPPMAX clusters.
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## Getting help
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We have a Slack channel dedicated to UPPMAX users on the nf-core Slack: [https://nfcore.slack.com/channels/uppmax](https://nfcore.slack.com/channels/uppmax)
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## Using the UPPMAX config profile
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Before running the pipeline you will need to either install `Nextflow` or load it using the environment module system (this can be done with e.g. `module load bioinfo-tools Nextflow/<VERSION>` where `VERSION` is e.g. `20.10`).
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To use, run the pipeline with `-profile uppmax` (one hyphen).
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This will download and launch the [`uppmax.config`](../conf/uppmax.config) which has been pre-configured with a setup suitable for the UPPMAX servers.
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It will enable `Nextflow` to manage the pipeline jobs via the `Slurm` job scheduler.
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Using this profile, `Docker` image(s) containing required software(s) will be downloaded, and converted to `Singularity` image(s) if needed before execution of the pipeline.
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Recent version of `Nextflow` also support the environment variable `NXF_SINGULARITY_CACHEDIR` which can be used to supply images.
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Images for some `nf-core` pipelines are available under `/sw/data/ToolBox/nf-core/` and those can be used by `NXF_SINGULARITY_CACHEDIR=/sw/data/ToolBox/nf-core/; export NXF_SINGULARITY_CACHEDIR`.
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In addition to this config profile, you will also need to specify an UPPMAX project id.
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You can do this with the `--project` flag (two hyphens) when launching `Nextflow`.
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For example:
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```bash
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# Launch a nf-core pipeline with the uppmax profile for the project id snic2018-1-234
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$ nextflow run nf-core/<PIPELINE> -profile uppmax --project snic2018-1-234 [...]
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```
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> NB: If you're not sure what your UPPMAX project ID is, try running `groups` or checking SUPR.
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Just run `Nextflow` on a login node and it will handle everything else.
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Remember to use `-bg` to launch `Nextflow` in the background, so that the pipeline doesn't exit if you leave your terminal session.
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Alternatively, you can also launch `Nextflow` in a `screen` or a `tmux` session.
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## Using AWS iGenomes references
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A local copy of the `AWS iGenomes` resource has been made available on all UPPMAX clusters so you should be able to run the pipeline against any reference available in the `conf/igenomes.config`.
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You can do this by simply using the `--genome <GENOME_ID>` parameter.
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## Getting more memory
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If your `nf-core` pipeline run is running out of memory, you can run on a fat node with more memory using the following `Nextflow` flags:
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```bash
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--clusterOptions "-C mem256GB -p node" --max_memory "256GB"
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```
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This raises the ceiling of available memory from the default of `128.GB` to `256.GB`.
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`rackham` has nodes with 128GB, 256GB and 1TB memory available.
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Note that each job will still start with the same request as normal, but restarted attempts with larger requests will be able to request greater amounts of memory.
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All jobs will be submitted to fat nodes using this method, so it's only for use in extreme circumstances.
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## Different UPPMAX clusters
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The UPPMAX nf-core configuration profile uses the `hostname` of the active environment to automatically apply the following resource limits:
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* `rackham`
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* cpus available: 20 cpus
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* memory available: 125 GB
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* `bianca`
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* cpus available: 16 cpus
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* memory available: 109 GB
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* `irma`
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* cpus available: 16 cpus
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* memory available: 250 GB
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## Development config
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If doing pipeline development work on UPPMAX, the `devel` profile allows for faster testing.
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Applied after main UPPMAX config, it overwrites certain parts of the config and submits jobs to the `devcore` queue, which has much faster queue times.
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All jobs are limited to 1 hour to be eligible for this queue and only one job allowed at a time.
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It is not suitable for use with real data.
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To use it, submit with `-profile uppmax,devel`.
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## Running on bianca
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> :warning: For more information, please follow the following guides:
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>
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> * [UPPMAX `bianca` user guide](http://uppmax.uu.se/support/user-guides/bianca-user-guide/).
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> * [nf-core guide for running offline](https://nf-co.re/usage/offline)
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> * [nf-core `tools` guide for downloading pipelines for offline use](https://nf-co.re/tools#downloading-pipelines-for-offline-use).
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> * [UPPMAX `Singularity` guide](https://www.uppmax.uu.se/support-sv/user-guides/singularity-user-guide/).
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For security reasons, there is no internet access on `bianca` so you can't download from or upload files to the cluster directly.
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Before running a nf-core pipeline on `bianca` you will first have to download the pipeline and singularity images needed elsewhere and transfer them via the `wharf` area to your own `bianca` project.
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In this guide, we use `rackham` to download and transfer files to the `wharf` area, but it can also be done on your own computer.
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If you use `rackham` to download the pipeline and the singularity containers, we recommend using an interactive session (cf [interactive guide](https://www.uppmax.uu.se/support/faq/running-jobs-faq/how-can-i-run-interactively-on-a-compute-node/)), which is what we do in the following guide.
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### Download and install Nextflow
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You can use the `Nextflow` UPPMAX provided `module`, but if necessary, you can also download a more recent version.
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```bash
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# Connect to bianca
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$ ssh -A <USER>-<BIANCA_PROJECT>@bianca.uppmax.uu.se
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# See the available versions for the module
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module spider Nextflow
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# Load a specific version of the Nextflow module
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module load bioinfo-tools Nextflow/<VERSION>`
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```
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```bash
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# Connect to rackham
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$ ssh -X <USER>@rackham.uppmax.uu.se
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# Or stay in your terminal
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# Download the nextflow-all bundle
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$ wget https://github.com/nextflow-io/nextflow/releases/download/v<NEXTFLOW_VERSION>/nextflow-<NEXTFLOW_VERSION>-all
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# Connect to the wharf area using sftp
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$ sftp <USER>-<BIANCA_PROJECT>@bianca-sftp.uppmax.uu.se:<USER>-<BIANCA_PROJECT>
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# Transfer nextflow to the wharf area
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sftp> put nextflow-<NEXTFLOW_VERSION>-all .
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# Exit sftp
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$ exit
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# Connect to bianca
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$ ssh -A <USER>-<BIANCA_PROJECT>@bianca.uppmax.uu.se
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# Go to your project
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$ cd /castor/project/proj_nobackup
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# Make folder for Nextflow
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$ mkdir tools
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$ mkdir tools/nextflow
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# Move Nextflow from the wharf area to its directory
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$ mv /castor/project/proj_nobackup/wharf/<USER>/<USER>-<BIANCA_PROJECT>/nextflow-<NEXTFLOW_VERSION>-all /castor/project/proj_nobackup/tools/nextflow
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# Establish permission
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$ chmod a+x /castor/project/proj_nobackup/tools/nextflow/nextflow-<NEXTFLOW_VERSION>-all
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# If you want other people to use it
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# Be sure that your group has rights to the directory as well
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$ chown -R .<BIANCA_PROJECT> /castor/project/proj_nobackup/tools/nextflow/nextflow-<NEXTFLOW_VERSION>-all
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# Make a link to it
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$ ln -s /castor/project/proj_nobackup/tools/nextflow/nextflow-<NEXTFLOW_VERSION>-all /castor/project/proj_nobackup/tools/nextflow/nextflow
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# And every time you're launching Nextflow, don't forget to export the following ENV variables
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# Or add them to your .bashrc file
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$ export NXF_HOME=/castor/project/proj/nobackup/tools/nextflow/
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$ export PATH=${NXF_HOME}:${PATH}
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$ export NXF_TEMP=$SNIC_TMP
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$ export NXF_LAUNCHER=$SNIC_TMP
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$ export NXF_SINGULARITY_CACHEDIR=/castor/project/proj_nobackup/singularity-images
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```
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### Install nf-core tools
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You can use the `nf-core` UPPMAX provided `module`, but if necessary, you can also download a more recent version.
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```bash
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# Connect to rackham
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$ ssh -X <USER>@rackham.uppmax.uu.se
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# See the available versions for the module
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module spider nf-core
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# Load a specific version of the nf-core module
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module load bioinfo-tools nf-core/<VERSION>`
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```
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```bash
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# Connect to rackham
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$ ssh -X <USER>@rackham.uppmax.uu.se
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# Or stay in your terminal
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# Install the latest pip version
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$ pip3 install --upgrade --force-reinstall git+https://github.com/nf-core/tools.git@dev --user
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```
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### Download and transfer a nf-core pipeline
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```bash
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# Connect to rackham
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$ ssh -X <USER>@rackham.uppmax.uu.se
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# Or stay in your terminal
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# Open an interactive session (if you are on rackham)
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$ interactive <rackham_project>
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# Download a pipeline with the singularity images
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$ nf-core download <PIPELINE> -r <PIPELINE_VERSION> -s --compress none
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# If necessary, extra singularity images can be download separately
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# For example, if you downloaded nf-core/sarek, you will need extra images for annotation
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# Here we download the nf-core/sarek GRCh38 specific images
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$ singularity pull --name nfcore-sareksnpeff-2.7.GRCh38.img docker://nfcore/sareksnpeff:2.7.GRCh38
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$ singularity pull --name nfcore-sarekvep-2.7.GRCh38.img docker://nfcore/sarekvep:2.7.GRCh38
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# Which can then be moved into the nf-core/sarek download folder
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$ mv *.img nf-core-sarek-2.7/singularity-images/.
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# Connect to the wharf area using sftp
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$ sftp <USER>-<BIANCA_PROJECT>@bianca-sftp.uppmax.uu.se:<USER>-<BIANCA_PROJECT>
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# Transfer <PIPELINE> folder from rackham to the wharf area
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sftp> put -r nf-core-<PIPELINE>-<PIPELINE_VERSION> .
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# The archives will be in the wharf folder in your user home on your bianca project
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# Connect to bianca
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$ ssh -A <USER>-<BIANCA_PROJECT>@bianca.uppmax.uu.se
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# Go to your project
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$ cd /castor/project/proj_nobackup
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# Make and go into a nf-core directory (where you will store all nf-core pipelines')
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$ mkdir nf-core
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$ cd nf-core
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# Move the folder from the wharf area to the project
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$ cp /castor/project/proj_nobackup/wharf/<USER>/<USER>-<BIANCA_PROJECT>/nf-core-<PIPELINE>-<PIPELINE_VERSION> .
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# If you want other people to use it,
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# Be sure that your group has rights to the directory as well
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$ chown -R .<BIANCA_PROJECT> nf-core-<PIPELINE>-<PIPELINE_VERSION>
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# Make a symbolic link to the extracted repository
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$ ln -s nf-core-<PIPELINE>-<PIPELINE_VERSION> nf-core-<PIPELINE>-default
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```
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The principle is to have every member of your project to be able to use the same `nf-core/<PIPELINE>` version at the same time.
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So every member of the project who wants to use `nf-core/<PIPELINE>` will need to do:
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```bash
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# Connect to bianca
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$ ssh -A <USER>-<BIANCA_PROJECT>@bianca.uppmax.uu.se
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# Go to your user directory
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$ cd /home/<USER>
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# Make a symbolic link to the default nf-core/<PIPELINE>
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$ ln -s /castor/project/proj_nobackup/nf-core/nf-core-<PIPELINE>-default nf-core-<PIPELINE>
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```
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And then `nf-core/<PIPELINE>` can be used with:
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```bash
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# run <PIPELINE> on bianca
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$ nextflow run ~/<PIPELINE> -profile uppmax --project <BIANCA_PROJECT> --genome <GENOME_ASSEMBLY> ...
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```
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## Update a pipeline
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To update, repeat the same steps as for installing and update the link.
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```bash
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# Connect to bianca (Connect to rackham first if needed)
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$ ssh -A <USER>-<BIANCA_PROJECT>@bianca.uppmax.uu.se
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# Go to the nf-core directory in your project
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$ cd /castor/project/proj_nobackup/nf-core
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# Remove link
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$ unlink nf-core-<PIPELINE>-default
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# Link to new nf-core/<PIPELINE> version
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$ ln -s nf-core-<PIPELINE>-<PIPELINE_VERSION> nf-core-<PIPELINE>-default
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```
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You can for example keep a `nf-core-<PIPELINE>-default` version that you are sure is working, an make a link for a `nf-core-<PIPELINE>-testing` or `nf-core-<PIPELINE>-development`.
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