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#!/usr/bin/env nextflow
nextflow.enable.dsl = 2
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include { BISMARK_GENOMEPREPARATION } from '../../../../software/bismark/genomepreparation/main.nf' addParams( options: [:] )
include { BISMARK_ALIGN as BISMARK_ALIGN_SE } from '../../../../software/bismark/align/main.nf' addParams( options: [ publish_dir:'test_single_end' ] )
include { BISMARK_ALIGN as BISMARK_ALIGN_PE } from '../../../../software/bismark/align/main.nf' addParams( options: [ publish_dir:'test_paired_end' ] )
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/*
* Test with single-end data
*/
workflow test_bismark_align_single_end {
input = [ [ id:'test', single_end:true ], // meta map
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[ file("${launchDir}/tests/data/genomics/sarscov2/illumina/fastq/test_methylated_1.fastq.gz", checkIfExists: true) ]
]
fasta = file("${launchDir}/tests/data/genomics/sarscov2/genome/genome.fasta", checkIfExists: true)
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BISMARK_GENOMEPREPARATION ( fasta )
BISMARK_ALIGN_SE ( input, BISMARK_GENOMEPREPARATION.out.index )
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}
/*
* Test with paired-end data
*/
workflow test_bismark_align_paired_end {
input = [ [ id:'test', single_end:false ], // meta map
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[ file("${launchDir}/tests/data/genomics/sarscov2/illumina/fastq/test_methylated_1.fastq.gz", checkIfExists: true),
file("${launchDir}/tests/data/genomics/sarscov2/illumina/fastq/test_methylated_2.fastq.gz", checkIfExists: true) ]
]
fasta = file("${launchDir}/tests/data/genomics/sarscov2/genome/genome.fasta", checkIfExists: true)
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BISMARK_GENOMEPREPARATION ( fasta )
BISMARK_ALIGN_PE ( input, BISMARK_GENOMEPREPARATION.out.index )
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}