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name : ivar_variants
description : Call variants from a BAM file using iVar
keywords :
- amplicon sequencing
- variants
- fasta
tools :
- ivar :
description : |
iVar - a computational package that contains functions broadly useful for viral amplicon-based sequencing.
homepage : https://github.com/andersen-lab/ivar
documentation : https://andersen-lab.github.io/ivar/html/manualpage.html
input :
- meta :
type : map
description : |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
- bam :
type : file
description : A sorted (with samtools sort) and trimmed (with iVar trim) bam file
pattern : "*.bam"
- fasta :
type : file
description : The reference sequence used for mapping and generating the BAM file
pattern : "*.fa"
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- gff :
type : file
description : A GFF file in the GFF3 format can be supplied to specify coordinates of open reading frames (ORFs). In absence of GFF file, amino acid translation will not be done.
patter : "*.gff"
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output :
- meta :
type : map
description : |
Groovy Map containing sample information
e.g. [ id:'test', single_end:false ]
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- tsv :
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type : file
description : iVar generated TSV file with the variants
pattern : "*.tsv"
- mpileup :
type : file
description : mpileup output from samtools mpileup [OPTIONAL]
pattern : "*.mpileup"
- version :
type : file
description : File containing software version
pattern : "*.{version.txt}"
authors :
- "@andersgs"
- "@drpatelh"