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luslab-umitools | Initialized test.nf
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#!/usr/bin/env nextflow
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// Define DSL2
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nextflow.preview.dsl=2
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// Log
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log.info ("Starting test pipeline for BAM deduplication")
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/* Module inclusions
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--------------------------------------------------------------------------------------*/
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include umi_tools from './umi-tools.nf'
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/*------------------------------------------------------------------------------------*/
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/* Define input channels
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--------------------------------------------------------------------------------------*/
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testMetaDataBam = [
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['Sample1', "$baseDir/input/prpf8_ctrl_rep1.Aligned.sortedByCoord.out.bam"],
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['Sample2', "$baseDir/input/prpf8_ctrl_rep2.Aligned.sortedByCoord.out.bam"],
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['Sample3', "$baseDir/input/prpf8_ctrl_rep4.Aligned.sortedByCoord.out.bam"],
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['Sample4', "$baseDir/input/prpf8_eif4a3_rep1.Aligned.sortedByCoord.out.bam"],
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['Sample5', "$baseDir/input/prpf8_eif4a3_rep2.Aligned.sortedByCoord.out.bam"],
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['Sample6', "$baseDir/input/prpf8_eif4a3_rep4.Aligned.sortedByCoord.out.bam"]
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]
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testMetaDataBai = [
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['Sample1', "$baseDir/input/prpf8_ctrl_rep1.Aligned.sortedByCoord.out.bai"],
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['Sample2', "$baseDir/input/prpf8_ctrl_rep2.Aligned.sortedByCoord.out.bai"],
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['Sample3', "$baseDir/input/prpf8_ctrl_rep4.Aligned.sortedByCoord.out.bai"],
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['Sample4', "$baseDir/input/prpf8_eif4a3_rep1.Aligned.sortedByCoord.out.bai"],
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['Sample5', "$baseDir/input/prpf8_eif4a3_rep2.Aligned.sortedByCoord.out.bai"],
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['Sample6', "$baseDir/input/prpf8_eif4a3_rep4.Aligned.sortedByCoord.out.bai"]
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]
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// Create channels of test data
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//Bam input channel
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Channel
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.from(testMetaDataBam)
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.map { row -> [ row[0], file(row[1], checkIfExists: true) ]}
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.set {ch_test_meta_bam}
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//BamBai input channel
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Channel
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.from(testMetaDataBai)
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.map { row -> [ row[0], file(row[1], checkIfExists: true) ] }
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.join(ch_test_meta_bam)
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.set {ch_test_meta_bambai}
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// Run workflow
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workflow {
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// Run dedup
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umi_tools( ch_test_meta_bambai )
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// Collect file names and view output
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umi_tools.out.dedupBam | view
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}
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