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Add picard/sortvcf (#1370)
* sortvcf * add files * update meta * update java mem * update documentation link * remove todo * review suggestions * fix test.yml * fix conda error * fix version code
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49
modules/picard/sortvcf/main.nf
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49
modules/picard/sortvcf/main.nf
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process PICARD_SORTVCF {
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tag "$meta.id"
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label 'process_medium'
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conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
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container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
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'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
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'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
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input:
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tuple val(meta), path(vcf)
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path reference
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path sequence_dict
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output:
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tuple val(meta), path("*_sorted.vcf.gz"), emit: vcf
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path "versions.yml" , emit: versions
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when:
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task.ext.when == null || task.ext.when
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script:
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def args = task.ext.args ?: ''
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def prefix = task.ext.prefix ?: "${meta.id}"
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def seq_dict = sequence_dict ? "-SEQUENCE_DICTIONARY $sequence_dict" : ""
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def reference = reference ? "-REFERENCE_SEQUENCE $reference" : ""
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def avail_mem = 3
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if (!task.memory) {
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log.info '[Picard SortVcf] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
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} else {
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avail_mem = task.memory.giga
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}
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"""
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picard \\
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SortVcf \\
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-Xmx${avail_mem}g \\
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--INPUT $vcf \\
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$args \\
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$seq_dict \\
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$reference \\
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--OUTPUT ${prefix}_sorted.vcf.gz
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cat <<-END_VERSIONS > versions.yml
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"${task.process}":
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picard: \$(picard SortVcf --version 2>&1 | grep -o 'Version:.*' | cut -f2- -d:)
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END_VERSIONS
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"""
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}
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40
modules/picard/sortvcf/meta.yml
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modules/picard/sortvcf/meta.yml
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name: picard_sortvcf
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description: Sorts vcf files
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keywords:
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- sort
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- vcf
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tools:
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- picard:
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description: Java tools for working with NGS data in the BAM/CRAM/SAM and VCF format
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homepage: https://broadinstitute.github.io/picard/
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documentation: https://broadinstitute.github.io/picard/command-line-overview.html#SortVcf
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licence: ['MIT']
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input:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- vcf:
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type: file
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description: VCF file
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pattern: "*.{vcf,vcf.gz}"
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output:
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- meta:
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type: map
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description: |
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Groovy Map containing sample information
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e.g. [ id:'test', single_end:false ]
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- versions:
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type: file
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description: File containing software versions
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pattern: "versions.yml"
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- vcf:
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type: file
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description: Sorted VCF file
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pattern: "*.{vcf}"
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authors:
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- "@ramprasadn"
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@ -1249,6 +1249,10 @@ picard/sortsam:
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- modules/picard/sortsam/**
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- tests/modules/picard/sortsam/**
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picard/sortvcf:
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- modules/picard/sortvcf/**
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- tests/modules/picard/sortvcf/**
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pirate:
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- modules/pirate/**
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- tests/modules/pirate/**
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18
tests/modules/picard/sortvcf/main.nf
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tests/modules/picard/sortvcf/main.nf
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#!/usr/bin/env nextflow
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nextflow.enable.dsl = 2
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include { PICARD_SORTVCF } from '../../../../modules/picard/sortvcf/main.nf'
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workflow test_picard_sortvcf {
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input = [ [ id:'test' ], // meta map
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file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true)
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]
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fasta = [ file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ]
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dict = [ file(params.test_data['sarscov2']['genome']['genome_dict'], checkIfExists: true) ]
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PICARD_SORTVCF ( input, fasta, dict )
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}
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5
tests/modules/picard/sortvcf/nextflow.config
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5
tests/modules/picard/sortvcf/nextflow.config
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process {
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publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
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}
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7
tests/modules/picard/sortvcf/test.yml
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7
tests/modules/picard/sortvcf/test.yml
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- name: picard sortvcf
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command: nextflow run ./tests/modules/picard/sortvcf -entry test_picard_sortvcf -c ./tests/config/nextflow.config -c ./tests/modules/picard/sortvcf/nextflow.config
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tags:
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- picard
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- picard/sortvcf
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files:
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- path: output/picard/test_sorted.vcf.gz
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