mirror of
https://github.com/MillironX/nf-core_modules.git
synced 2024-12-22 19:18:17 +00:00
55 lines
1.7 KiB
Text
55 lines
1.7 KiB
Text
process UMITOOLS_EXTRACT {
|
|
tag "$meta.id"
|
|
label "process_low"
|
|
|
|
conda (params.enable_conda ? "bioconda::umi_tools=1.1.2" : null)
|
|
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
|
'https://depot.galaxyproject.org/singularity/umi_tools:1.1.2--py38h4a8c8d9_0' :
|
|
'quay.io/biocontainers/umi_tools:1.1.2--py38h4a8c8d9_0' }"
|
|
|
|
input:
|
|
tuple val(meta), path(reads)
|
|
|
|
output:
|
|
tuple val(meta), path("*.fastq.gz"), emit: reads
|
|
tuple val(meta), path("*.log") , emit: log
|
|
path "versions.yml" , emit: versions
|
|
|
|
when:
|
|
task.ext.when == null || task.ext.when
|
|
|
|
script:
|
|
def args = task.ext.args ?: ''
|
|
def prefix = task.ext.prefix ?: "${meta.id}"
|
|
if (meta.single_end) {
|
|
"""
|
|
umi_tools \\
|
|
extract \\
|
|
-I $reads \\
|
|
-S ${prefix}.umi_extract.fastq.gz \\
|
|
$args \\
|
|
> ${prefix}.umi_extract.log
|
|
|
|
cat <<-END_VERSIONS > versions.yml
|
|
"${task.process}":
|
|
umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
|
|
END_VERSIONS
|
|
"""
|
|
} else {
|
|
"""
|
|
umi_tools \\
|
|
extract \\
|
|
-I ${reads[0]} \\
|
|
--read2-in=${reads[1]} \\
|
|
-S ${prefix}.umi_extract_1.fastq.gz \\
|
|
--read2-out=${prefix}.umi_extract_2.fastq.gz \\
|
|
$args \\
|
|
> ${prefix}.umi_extract.log
|
|
|
|
cat <<-END_VERSIONS > versions.yml
|
|
"${task.process}":
|
|
umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//')
|
|
END_VERSIONS
|
|
"""
|
|
}
|
|
}
|