Add rotavirus paper
Signed-off-by: Thomas A. Christensen II <25492070+MillironX@users.noreply.github.com>
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content/academia/rotavirus-virome.md
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content/academia/rotavirus-virome.md
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---
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title:
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"Assessment of Porcine Rotavirus-associated virome variations in pigs with
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enteric disease"
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date: 2022-04-27
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cardImage: cannulated-cows
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draft: true
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featured: true
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keywords:
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- porcine rotavirus
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- porcine enteric disease
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- virome
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- rotavirus
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type: paper
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authors:
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- Tyler Doerksen
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- Thomas A. Christensen II
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- Andrea Lu
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- Lance Noll
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- Jianfa Bai
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- Jamie Henningson
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- Rachel Palinski
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link: https://doi.org/10.1016/j.vetmic.2022.109447
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journal: Veterinary Microbiology
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---
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Enteric disease is the predominant cause of morbidity and mortality in young
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mammals including pigs. Viral species involved in porcine enteric disease
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complex (PEDC) include rotaviruses, coronaviruses, picornaviruses, astroviruses
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and pestiviruses among others. The virome of three groups of swine samples
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submitted to the Kansas State University Veterinary Diagnostic Laboratory for
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routine testing were assessed, namely, a Rotavirus A positive (RVA) group, a
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Rotavirus co-infection (RV) group and a Rotavirus Negative (RV Neg) group. All
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groups were designated by qRT-PCR results testing for Porcine Rotavirus A, B, C
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and H such that samples positive for RVA only went in the RVA group, samples
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positive for >1 rotavirus went in the RV group and samples negative for all were
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grouped in the RVNeg group. All of the animals had clinical enteric disease
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resulting in scours and swollen joints/lameness, enlarged heart and/or a cough.
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All samples were metagenomic sequenced and analyzed for viral species
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composition that identified 14 viral species and eight bacterial viruses/phages.
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Sapovirus and Escherichia coli phages were found at a high prevalence in RVA and
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RV samples but were found at low or no prevalence in the RV Neg samples.
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Picobirnavirus was identified at a high proportion and prevalence in RV Neg and
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RV samples but at a low prevalence in the RVA group. A sequence analysis of the
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possible host of Picobirnaviruses revealed fungi as the most likely host.
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Non-rotaviral diversity was highest in RVA samples followed by RV then RV Neg
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samples. Various sequences were extracted from the sample reads and a
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phylogenetic update was provided showing a high prevalence of G9 and P[23] RVA
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genotypes. These data are important for pathogen surveillance and control
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measures
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