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<!doctype html><html class=no-js lang=en><head><meta charset=utf-8><meta http-equiv=x-ua-compatible content="ie=edge"><meta name=viewport content="width=device-width,initial-scale=1"><title>Assessment of Porcine Rotavirus-associated virome variations in pigs with enteric disease - MillironX</title><link href="/styles/mix-twbs.min.css" rel=stylesheet></head><body><div class=container-fluid><div class="row wrapper min-vh-100 flex-column flex-sm-row"><aside class="col-12 col-md-3 p-0 bg-dark flex-shrink-1"><nav class="navbar navbar-expand-md navbar-dark bg-dark align-items-start flex-md-column flex-row"><div class=container-fluid><a class="navbar-brand d-block d-md-none" href=#><object class="d-inline-block align-text-top" width=80 height=24 style=filter:invert(100%) data=/graphics/millironx.svg>
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  Milliron X</h1></header></div><section class="container-fluid list-main"><div class="container px-5"><h5>Veterinary Microbiology</h5><h2>Assessment of Porcine Rotavirus-associated virome variations in pigs with enteric disease</h2><h3><small><ul class=list-inline></ul></small></h3><h4>April 27, 2022</h4><p>Enteric disease is the predominant cause of morbidity and mortality in young
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mammals including pigs. Viral species involved in porcine enteric disease
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complex (PEDC) include rotaviruses, coronaviruses, picornaviruses, astroviruses
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and pestiviruses among others. The virome of three groups of swine samples
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submitted to the Kansas State University Veterinary Diagnostic Laboratory for
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routine testing were assessed, namely, a Rotavirus A positive (RVA) group, a
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Rotavirus co-infection (RV) group and a Rotavirus Negative (RV Neg) group. All
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groups were designated by qRT-PCR results testing for Porcine Rotavirus A, B, C
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and H such that samples positive for RVA only went in the RVA group, samples
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positive for >1 rotavirus went in the RV group and samples negative for all were
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grouped in the RVNeg group. All of the animals had clinical enteric disease
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resulting in scours and swollen joints/lameness, enlarged heart and/or a cough.
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All samples were metagenomic sequenced and analyzed for viral species
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composition that identified 14 viral species and eight bacterial viruses/phages.
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Sapovirus and Escherichia coli phages were found at a high prevalence in RVA and
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RV samples but were found at low or no prevalence in the RV Neg samples.
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Picobirnavirus was identified at a high proportion and prevalence in RV Neg and
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RV samples but at a low prevalence in the RVA group. A sequence analysis of the
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possible host of Picobirnaviruses revealed fungi as the most likely host.
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Non-rotaviral diversity was highest in RVA samples followed by RV then RV Neg
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samples. Various sequences were extracted from the sample reads and a
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phylogenetic update was provided showing a high prevalence of G9 and P[23] RVA
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genotypes. These data are important for pathogen surveillance and control
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measures</p><div class="card border-dark m-3 p-3"><a href=https://doi.org/10.1016/j.vetmic.2022.109447>https://doi.org/10.1016/j.vetmic.2022.109447</a>
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purpose.</p></div><div class="tab-pane fade p-3" id=debug role=tabpanel><dl><dt>cardimage</dt><dd>cannulated-cows</dd><dt>categories</dt><dd>[paper]</dd><dt>date</dt><dd>2022-04-27 00:00:00 +0000 UTC</dd><dt>draft</dt><dd>false</dd><dt>featured</dt><dd>true</dd><dt>iscjklanguage</dt><dd>false</dd><dt>journal</dt><dd>Veterinary Microbiology</dd><dt>lastmod</dt><dd>2022-04-27 00:00:00 +0000 UTC</dd><dt>link</dt><dd>https://doi.org/10.1016/j.vetmic.2022.109447</dd><dt>people</dt><dd>[Tyler Doerksen Thomas A. Christensen II Andrea Lu Lance Noll Jianfa Bai Jamie Henningson Rachel Palinski]</dd><dt>publishdate</dt><dd>2022-04-27 00:00:00 +0000 UTC</dd><dt>tags</dt><dd>[porcine rotavirus porcine enteric disease virome rotavirus]</dd><dt>title</dt><dd>Assessment of Porcine Rotavirus-associated virome variations in pigs with enteric disease</dd></dl></div></div></div></div></div></div></body></html> |