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<!doctype html><html class=no-js lang=en><head><meta charset=utf-8><meta http-equiv=x-ua-compatible content="ie=edge"><meta name=viewport content="width=device-width,initial-scale=1"><title>Thomas A. Christensen II - MillironX</title><link href="/styles/mix-twbs.min.css" rel=stylesheet></head><body><div class=container-fluid><div class="row wrapper min-vh-100 flex-column flex-sm-row"><aside class="col-12 col-md-3 p-0 bg-dark flex-shrink-1"><nav class="navbar navbar-expand-md navbar-dark bg-dark align-items-start flex-md-column flex-row"><div class=container-fluid><a class="navbar-brand d-block d-md-none" href=#><object class="d-inline-block align-text-top" width=80 height=24 style=filter:invert(100%) data=/graphics/millironx.svg>
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&emsp; Milliron X</h1></header></div><div class=blurred-container><div class=motto><h1 id=motto>Thomas A. Christensen II</h1></div><div class=img-src style=background-image:url(/images/saddles.jpg)></div><div class="img-src blur" style=background-image:url(/images/saddles_hu1143faa57f5b1acd11a97eda612b56ee_388130_filter_6742909828560968691.jpg)></div></div><br><section class="container-fluid list-main"><div class="container px-5"></div><div class=row data-masonry='{"percentPosition": true}'><div class="col-xl-6 mb-4"><div class=card><div class=category-button><a href=/categories/paper/ class="btn btn-dark btn-sm" data-bs-toggle=tooltip title=Paper><i class="fad fa-book fa-fw"></i></a></div><div class=card-body><a href=https://doi.org/10.1101/2023.10.20.563221><h3 class=card-title>nf-core/taxprofiler: highly parallelised and flexible pipeline for metagenomic taxonomic classification and profiling</h3></a><div>23 Oct 2023</div><a href=/people/sofia-stamouli/ class="icon-link card-link"><i class="fad fa-user"></i> Sofia Stamouli</a>
<a href=/people/moritz-e.-beber/ class="icon-link card-link"><i class="fad fa-user"></i> Moritz E. Beber</a>
<a href=/people/tanja-normark/ class="icon-link card-link"><i class="fad fa-user"></i> Tanja Normark</a>
<a href=/people/thomas-a.-christensen-ii/ class="icon-link card-link
fw-bolder"><i class="fad fa-user"></i> Thomas A. Christensen II</a>
<a href=/people/lili-andersson-li/ class="icon-link card-link"><i class="fad fa-user"></i> Lili Andersson-Li</a>
<a href=/people/maxime-borry/ class="icon-link card-link"><i class="fad fa-user"></i> Maxime Borry</a>
<a href=/people/mahwash-jamy/ class="icon-link card-link"><i class="fad fa-user"></i> Mahwash Jamy</a>
<a href=/people/nf-core-community/ class="icon-link card-link"><i class="fad fa-user"></i> nf-core community</a>
<a href=/people/james-a.-fellows-yate/ class="icon-link card-link"><i class="fad fa-user"></i> James A. Fellows Yate</a><p class=card-text>Metagenomic classification tackles the problem of characterising the taxonomic source of all DNA sequencing reads in a sample. A common approach to address the differences and biases between the many different taxonomic classification tools is to run metagenomic data through multiple classification tools and databases. This, however, is a very time-consuming task when performed manually - particularly when combined with the appropriate preprocessing of sequencing reads before the classification. Here we present nf-core/taxprofiler, a highly parallelised read-processing and taxonomic classification pipeline.
<strong><small><a href=https://doi.org/10.1101/2023.10.20.563221>Read&nbsp;more &#187;</a></small></strong></p></div><div class=card-footer><a href=/tags/genomics/ class="icon-link card-link"><i class="fad fa-tag"></i> genomics</a></div></div></div><div class="col-xl-6 mb-4"><div class=card><div class=category-button><a href=/categories/paper/ class="btn btn-dark btn-sm" data-bs-toggle=tooltip title=Paper><i class="fad fa-book fa-fw"></i></a></div><div class=card-body><a href=https://doi.org/10.1021/acsestengg.2c00107><h3 class=card-title>Investigation of Hydronium Diffusion in Poly(vinyl alcohol) Hydrogels: A Critical First Step to Describe Acid Transport for Encapsulated Bioremediation</h3></a><div>02 Sep 2022</div><a href=/people/carson-j.-silsby/ class="icon-link card-link"><i class="fad fa-user"></i> Carson J. Silsby</a>
<a href=/people/jonathan-r.-counts/ class="icon-link card-link"><i class="fad fa-user"></i> Jonathan R. Counts</a>
<a href=/people/thomas-a.-christensen-ii/ class="icon-link card-link
fw-bolder"><i class="fad fa-user"></i> Thomas A. Christensen II</a>
<a href=/people/mark-f.-roll/ class="icon-link card-link"><i class="fad fa-user"></i> Mark F. Roll</a>
<a href=/people/kristopher-v.-waynant/ class="icon-link card-link"><i class="fad fa-user"></i> Kristopher V. Waynant</a>
<a href=/people/james-g.-moberly/ class="icon-link card-link"><i class="fad fa-user"></i> James G. Moberly</a><p class=card-text>Bioremediation of chlorinated aliphatic hydrocarbon-contaminated aquifers can be hindered by high contaminant concentrations and acids generated during remediation. Encapsulating microbes in hydrogels may provide a protective, tunable environment from inhibiting compounds; however, current approaches to formulate successful encapsulated systems rely on trial and error rather than engineering approaches because fundamental information on mass-transfer coefficients is lacking. To address this knowledge gap, hydronium ion mass-transfer rates through two commonly used hydrogel materials, poly(vinyl alcohol) and alginic acid, under two solidification methods (chemical and cryogenic) were measured.
<strong><small><a href=https://doi.org/10.1021/acsestengg.2c00107>Read&nbsp;more &#187;</a></small></strong></p></div><div class=card-footer><a href=/tags/diffusion/ class="icon-link card-link"><i class="fad fa-tag"></i> diffusion</a>
<a href=/tags/hydrogels/ class="icon-link card-link"><i class="fad fa-tag"></i> hydrogels</a>
<a href=/tags/ionic-strength/ class="icon-link card-link"><i class="fad fa-tag"></i> ionic strength</a>
<a href=/tags/polymers/ class="icon-link card-link"><i class="fad fa-tag"></i> polymers</a>
<a href=/tags/transport-properties/ class="icon-link card-link"><i class="fad fa-tag"></i> transport properties</a></div></div></div><div class="col-xl-6 mb-4"><div class=card><div class=category-button><a href=/categories/paper/ class="btn btn-dark btn-sm" data-bs-toggle=tooltip title=Paper><i class="fad fa-book fa-fw"></i></a></div><div class=card-body><a href=https://doi.org/10.1016/j.vetmic.2022.109447><h3 class=card-title>Assessment of Porcine Rotavirus-associated virome variations in pigs with enteric disease</h3></a><div>27 Apr 2022</div><a href=/people/tyler-doerksen/ class="icon-link card-link"><i class="fad fa-user"></i> Tyler Doerksen</a>
<a href=/people/thomas-a.-christensen-ii/ class="icon-link card-link
fw-bolder"><i class="fad fa-user"></i> Thomas A. Christensen II</a>
<a href=/people/andrea-lu/ class="icon-link card-link"><i class="fad fa-user"></i> Andrea Lu</a>
<a href=/people/lance-noll/ class="icon-link card-link"><i class="fad fa-user"></i> Lance Noll</a>
<a href=/people/jianfa-bai/ class="icon-link card-link"><i class="fad fa-user"></i> Jianfa Bai</a>
<a href=/people/jamie-henningson/ class="icon-link card-link"><i class="fad fa-user"></i> Jamie Henningson</a>
<a href=/people/rachel-palinski/ class="icon-link card-link"><i class="fad fa-user"></i> Rachel Palinski</a><p class=card-text>Enteric disease is the predominant cause of morbidity and mortality in young mammals including pigs. Viral species involved in porcine enteric disease complex (PEDC) include rotaviruses, coronaviruses, picornaviruses, astroviruses and pestiviruses among others. The virome of three groups of swine samples submitted to the Kansas State University Veterinary Diagnostic Laboratory for routine testing were assessed, namely, a Rotavirus A positive (RVA) group, a Rotavirus co-infection (RV) group and a Rotavirus Negative (RV Neg) group.
<strong><small><a href=https://doi.org/10.1016/j.vetmic.2022.109447>Read&nbsp;more &#187;</a></small></strong></p></div><div class=card-footer><a href=/tags/porcine-rotavirus/ class="icon-link card-link"><i class="fad fa-tag"></i> porcine rotavirus</a>
<a href=/tags/porcine-enteric-disease/ class="icon-link card-link"><i class="fad fa-tag"></i> porcine enteric disease</a>
<a href=/tags/virome/ class="icon-link card-link"><i class="fad fa-tag"></i> virome</a>
<a href=/tags/rotavirus/ class="icon-link card-link"><i class="fad fa-tag"></i> rotavirus</a></div></div></div><div class="col-xl-6 mb-4"><div class=card><div class=category-button><a href=/categories/thesis/ class="btn btn-dark btn-sm" data-bs-toggle=tooltip title=Thesis><i class="fad fa-graduation-cap fa-fw"></i></a></div><div class=card-body><a href=https://www.proquest.com/dissertations-theses/polyoxometalate-incorporation-effects-on-proton/docview/2502214356/se-2><h3 class=card-title>Polyoxometalate Incorporation and Effects on Proton Transport in Hydrogel Polymers</h3></a><div>07 Aug 2020</div><a href=/people/thomas-a.-christensen-ii/ class="icon-link card-link
fw-bolder"><i class="fad fa-user"></i> Thomas A. Christensen II</a><p class=card-text>Polyoxometalate clusters embedded into hydrogel biobeads may be able to solve the challenges posed by free proton generation during remediation of trichloroethylene by acting as buffers and reducing protons to hydrogen gas. In this thesis, the challenges posed by systems that contain both diffusion and reaction processes for protons are considered mathematically, and a computer simulation to was developed to prove the relationship between diaphragm cell lag period and reactive capabilities of membranes.
<strong><small><a href=https://www.proquest.com/dissertations-theses/polyoxometalate-incorporation-effects-on-proton/docview/2502214356/se-2>Read&nbsp;more &#187;</a></small></strong></p></div><div class=card-footer><a href=/tags/bioremediation/ class="icon-link card-link"><i class="fad fa-tag"></i> bioremediation</a>
<a href=/tags/polyoxometalate/ class="icon-link card-link"><i class="fad fa-tag"></i> polyoxometalate</a>
<a href=/tags/hydrogel-polymers/ class="icon-link card-link"><i class="fad fa-tag"></i> hydrogel polymers</a>
<a href=/tags/proton-transport/ class="icon-link card-link"><i class="fad fa-tag"></i> proton transport</a>
<a href=/tags/chemical-engineering/ class="icon-link card-link"><i class="fad fa-tag"></i> chemical engineering</a></div></div></div><div class="col-xl-6 mb-4"><div class=card><div class=category-button><a href=/categories/poster/ class="btn btn-dark btn-sm" data-bs-toggle=tooltip title=Poster><i class="fad fa-presentation fa-fw"></i></a></div><div class=card-body><a href=/academia/metagenomics/metagenomics_analysis_of_rumen_populations.pdf><h3 class=card-title>Metagenomic analysis of rumen populations in week-old calves as altered by maternal late gestational nutrition and mode of delivery</h3></a><div>12 Jun 2019</div><a href=/people/thomas-a.-christensen-ii/ class="icon-link card-link
fw-bolder"><i class="fad fa-user"></i> Thomas A. Christensen II</a>
<a href=/people/kathy-j.-austin/ class="icon-link card-link"><i class="fad fa-user"></i> Kathy J. Austin</a>
<a href=/people/kristi-m.-cammack/ class="icon-link card-link"><i class="fad fa-user"></i> Kristi M. Cammack</a>
<a href=/people/hannah-c.-cunningham-hollinger/ class="icon-link card-link"><i class="fad fa-user"></i> Hannah C. Cunningham-Hollinger</a><p class=card-text>Early colonization of the rumen microbiome is critical to host health and long term performance. Factors that influence early colonization include maternal factors such as gestational nutrition and mode of delivery. Therefore, we hypothesized that late gestational nutrition and mode of delivery would influence the calf rumen microbiome. Our objectives were to determine if nutrient restriction during late gestation alters the calf rumen microbiome and determine if ruminal microbiome composition differs in calves born vaginally versus caesarean.
<strong><small><a href=/academia/metagenomics/metagenomics_analysis_of_rumen_populations.pdf>Read&nbsp;more &#187;</a></small></strong></p></div><div class=card-footer><a href=/tags/gestation/ class="icon-link card-link"><i class="fad fa-tag"></i> gestation</a>
<a href=/tags/metagenomics/ class="icon-link card-link"><i class="fad fa-tag"></i> metagenomics</a>
<a href=/tags/microbiome/ class="icon-link card-link"><i class="fad fa-tag"></i> microbiome</a>
<a href=/tags/rumen/ class="icon-link card-link"><i class="fad fa-tag"></i> rumen</a></div></div></div><div class="col-xl-6 mb-4"><div class=card><div class=category-button><a href=/categories/thesis/ class="btn btn-dark btn-sm" data-bs-toggle=tooltip title=Thesis><i class="fad fa-graduation-cap fa-fw"></i></a></div><div class=card-body><img class="img img-thumbnail float-start me-3 md-max-width-33" src=/academia/cheme-car/thumbnail_hua27ceb9f6c1a8b01057b70de792ffbc6_1566619_600x0_resize_q75_box.jpg alt="Thumbnail of thumbnail.jpg">
<a href=https://doi.org/10.15786/13700938.v1><h3 class=card-title>The ChemE Car that Cud: AIChE ChemE Car Engineering Design Proposal</h3></a><div>14 May 2019</div><a href=/people/thomas-a.-christensen-ii/ class="icon-link card-link
fw-bolder"><i class="fad fa-user"></i> Thomas A. Christensen II</a><p class=card-text>The ChemE Car That Cud showcases Wyoming&rsquo;s dominant industries of agriculture and mining by utilizing rumen fluid from a cannulated beef cow to generate hydrogen to be used in a hydrogen fuel cell and radioactive cesium, a byproduct of uranium that is often obtained from Wyoming&rsquo;s mines, to time the car&rsquo;s stop. The concentration of cesium-137 source is measured using the radioactive decay of cesium shielded by aluminum. The painted aluminum chassis was obtained from a previous team at UW, and modified using plastic k&rsquo;nex toys to adapt to the current power source and stopping mechanism.
<strong><small><a href=https://doi.org/10.15786/13700938.v1>Read&nbsp;more &#187;</a></small></strong></p></div><div class=card-footer><a href=/tags/chemical-engineering/ class="icon-link card-link"><i class="fad fa-tag"></i> chemical engineering</a>
<a href=/tags/aiche/ class="icon-link card-link"><i class="fad fa-tag"></i> AIChE</a>
<a href=/tags/radiation/ class="icon-link card-link"><i class="fad fa-tag"></i> radiation</a>
<a href=/tags/rumen/ class="icon-link card-link"><i class="fad fa-tag"></i> rumen</a>
<a href=/tags/microbial-electrolysis-cells/ class="icon-link card-link"><i class="fad fa-tag"></i> microbial electrolysis cells</a></div></div></div><div class="col-xl-6 mb-4"><div class=card><div class=category-button><a href=/categories/poster/ class="btn btn-dark btn-sm" data-bs-toggle=tooltip title=Poster><i class="fad fa-presentation fa-fw"></i></a></div><div class=card-body><a href=/academia/pva-aiche/measuring_diffusion_of_trichloroethylene.pdf><h3 class=card-title>Measuring Diffusion of Trichlorethylene Breakdown Products in Polyvinylalginate</h3></a><div>29 Oct 2018</div><a href=/people/thomas-a.-christensen-ii/ class="icon-link card-link
fw-bolder"><i class="fad fa-user"></i> Thomas A. Christensen II</a>
<a href=/people/samuel-r.-wolfe/ class="icon-link card-link"><i class="fad fa-user"></i> Samuel R. Wolfe</a>
<a href=/people/jonathan-counts/ class="icon-link card-link"><i class="fad fa-user"></i> Jonathan Counts</a>
<a href=/people/mark-f.-roll/ class="icon-link card-link"><i class="fad fa-user"></i> Mark F. Roll</a>
<a href=/people/kristopher-v.-waynant/ class="icon-link card-link"><i class="fad fa-user"></i> Kristopher V. Waynant</a>
<a href=/people/james-g.-moberly/ class="icon-link card-link"><i class="fad fa-user"></i> James G. Moberly</a><p class=card-text>Trichloroethylene (TCE), a toxic and carcinogenic contaminant, presents unique challenges for cleanup because of its water solubility, density, and volatility. Bioremediation of TCE is a promising cleanup method; however, metabolism of TCE results in acid generation that inhibits remediating microorganisms. Calcium alginate(CA)-polyvinylalcohol (PVA) hydrogels show promise for protecting remediating microbes, however diffusion of TCE or its byproducts through these polymers is unknown. To measure the effective diffusion coefficient of TCE and byproducts through hydrogel membranes, we used a modified diaphragm cell.
<strong><small><a href=/academia/pva-aiche/measuring_diffusion_of_trichloroethylene.pdf>Read&nbsp;more &#187;</a></small></strong></p></div><div class=card-footer><a href=/tags/bioremediation/ class="icon-link card-link"><i class="fad fa-tag"></i> bioremediation</a>
<a href=/tags/polyoxometalate/ class="icon-link card-link"><i class="fad fa-tag"></i> polyoxometalate</a>
<a href=/tags/hydrogel-polymers/ class="icon-link card-link"><i class="fad fa-tag"></i> hydrogel polymers</a>
<a href=/tags/proton-transport/ class="icon-link card-link"><i class="fad fa-tag"></i> proton transport</a>
<a href=/tags/chemical-engineering/ class="icon-link card-link"><i class="fad fa-tag"></i> chemical engineering</a></div></div></div><div class="col-xl-6 mb-4"><div class=card><div class=category-button><a href=/categories/presentation/ class="btn btn-dark btn-sm" data-bs-toggle=tooltip title=Presentation><i class="fad fa-podium fa-fw"></i></a></div><div class=card-body><a href=/academia/how-to-build-a-cow-cud-fuel-cell/><h3 class=card-title>How to Build a Cow-Cud Fuel Cell</h3></a><div>01 Aug 2018</div><a href=/people/thomas-a.-christensen-ii/ class="icon-link card-link
fw-bolder"><i class="fad fa-user"></i> Thomas A. Christensen II</a><p class=card-text><strong><small><a href=/academia/how-to-build-a-cow-cud-fuel-cell/>Read&nbsp;more &#187;</a></small></strong></p></div><div class=card-footer></div></div></div><div class="col-xl-6 mb-4"><div class=card><div class=category-button><a href=/categories/poster/ class="btn btn-dark btn-sm" data-bs-toggle=tooltip title=Poster><i class="fad fa-presentation fa-fw"></i></a></div><div class=card-body><a href=/academia/pva-inbre/><h3 class=card-title>Measuring diffusion of protons in polyvinyalginate</h3></a><div>31 Jul 2018</div><a href=/people/thomas-a.-christensen-ii/ class="icon-link card-link
fw-bolder"><i class="fad fa-user"></i> Thomas A. Christensen II</a>
<a href=/people/jonathan-counts/ class="icon-link card-link"><i class="fad fa-user"></i> Jonathan Counts</a>
<a href=/people/james-g.-moberly/ class="icon-link card-link"><i class="fad fa-user"></i> James G. Moberly</a><p class=card-text>Trichloroethylene (TCE) is a toxic and carcinogenic contaminant that presents unique challenges for cleanup because of its density and volatility. Use of microorganisms may be a promising remediation method, however metabolism of TCE results in acid buildup, which consequently impedes the ability of microorganisms to perform this remediation. Polyvinylalginate (PVA) shows promise as a useful shield for microorganisms carrying out bioremediation of TCE by surrounding them in a protective biofilm-like layer, however, key information is missing which relates diffusion of TCE or its metabolic products through PVA.
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<a class="btn btn-dark" href=https://code.millironx.com/millironx/millironx.github.io/src/branch/master/LICENSE><i class="fad fa-scale-balanced"></i>&emsp;MIT Licensed &#187;</a></p><p>In addition, I used a number of excellent open-source libraries
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acknowledging them all.</p><table class="table table-condensed table-responsive table-striped"><tr><th>Project name</th><th>URL</th><th>License</th></tr><tr><th>Hugo</th><td><a href=https://gohugo.io/>gohugo.io</a></td><td><a href=https://github.com/gohugoio/hugo/blob/master/LICENSE>Apache License v2</a></td></tr><tr><th>Node.js</th><td><a href=https://nodejs.org/>nodejs.org</a></td><td><a href=https://github.com/nodejs/node/blob/HEAD/LICENSE>MIT License</a></td></tr><tr><th>Bootstrap 5</th><td><a href=https://getbootstrap.com/>getbootstrap.com</a></td><td><a href=https://github.com/twbs/bootstrap/blob/main/LICENSE>MIT License</a></td></tr><tr><th>Bootswatch Lux 5</th><td><a href=https://bootswatch.com/lux/>bootswatch.com</a></td><td><a href=https://github.com/thomaspark/bootswatch/blob/v5/LICENSE>MIT License</a></td></tr><tr><th>JQuery 3</th><td><a href=https://jquery.com/>jquery.com</a></td><td><a href=https://jquery.org/license>MIT License</a></td></tr><tr><th>Font Awesome 6</th><td><a href=https://fontawesome.com/>fontawesome.com</a></td><td><a href=https://fontawesome.com/license/>Font Awesome Pro License</a></td></tr><tr><th>Get S*** Done Toolkit</th><td><a href=https://www.creative-tim.com/product/get-shit-done-kit>creative-tim.com</a></td><td><a href=https://github.com/timcreative/freebies/blob/master/LICENSE.md>MIT License</a></td></tr><tr><th>FitText</th><td><a href=http://fittextjs.com/>fittextjs.com</a></td><td><a href=http://www.wtfpl.net/>WTFPL License</a></td></tr><tr><th>jQuery Mask Plugin</th><td><a href=https://igorescobar.github.io/jQuery-Mask-Plugin/>igorescobar.github.io</a></td><td><a href=https://github.com/igorescobar/jQuery-Mask-Plugin/blob/master/LICENSE>MIT License</a></td></tr><tr><th>PostCSS</th><td><a href=https://postcss.org/>postcss.com</a></td><td><a href=https://github.com/postcss/postcss/blob/main/LICENSE>MIT License</a></td></tr><tr><th>PurgeCSS</th><td><a href=https://purgecss.com/>purgecss.com</a></td><td><a href=https://github.com/FullHuman/purgecss/blob/main/LICENSE>MIT License</a></td></tr></table></div><div class="tab-pane fade p-3" id=privacy role=tabpanel><p>I take privacy very seriously. That said, I do need
<i>some</i> info on how many people visit. As a compromise, this
site uses <a href=https://goatcounter.com>GoatCounter</a>, a
privacy-friendly (as much as possible) web analytics library.</p><p>You may view all analytics gathered at
<a href=https://millironx.goatcounter.com/>millironx.goatcounter.com</a>.</p><p>If you do not wish to participate in my site's analytics, you may
install a content-blocking extension into your browser and block
the domain
<code>gc.zgo.at</code>. I recommend either
<a href=https://github.com/gorhill/uBlock>uBlock Origin</a>
(instructions
<a href=https://github.com/gorhill/uBlock/wiki/Dashboard:-My-filters>here</a>) or <a href=https://noscript.net>NoScript</a> for this
purpose.</p></div><div class="tab-pane fade p-3" id=debug role=tabpanel><dl><dt>draft</dt><dd>false</dd><dt>iscjklanguage</dt><dd>false</dd><dt>title</dt><dd>Thomas A. Christensen II</dd></dl></div></div></div></div></div></div></body></html>