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<!doctype html><html class=no-js lang=en><head><meta charset=utf-8><meta http-equiv=x-ua-compatible content="ie=edge"><meta name=viewport content="width=device-width,initial-scale=1"><title>Assessment of Porcine Rotavirus-associated virome variations in pigs with enteric disease - MillironX</title><link href="/styles/millironx.min.css" rel=stylesheet></head><body><header><object data=/graphics/millironx.svg>
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<img src=/graphics/millironx.svg alt="Milliron X"></object><h1 class=font-small-caps>Milliron X</h1></header><div class=row><aside><nav><a href=/><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 576 512"><path d="M511.8 287.6H576V240L288.4.0.0 240v47.6H64.1V512H224V352H352V512H512.8l-1-224.4z"/></svg></span>Home</a>
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<a href=/websites/><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path d="M0 32H512V480H0V32zm160 72v48H448V104H160zm-32-8H64v64h64V96z"/></svg></span>Websites</a></nav></aside><main><section><h5>Veterinary Microbiology</h5><h2>Assessment of Porcine Rotavirus-associated virome variations in pigs with enteric disease</h2><h3><small><a href=/people/tyler-doerksen/ class=card-link><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path d="M224 256A128 128 0 10224 0a128 128 0 100 256zM448 512 384 304H64L0 512H448z"/></svg></span>Tyler Doerksen</a>
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<a href=/people/thomas-a.-christensen-ii/ class="card-link
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bolder"><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path d="M224 256A128 128 0 10224 0a128 128 0 100 256zM448 512 384 304H64L0 512H448z"/></svg></span>Thomas A. Christensen II</a>
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<a href=/people/andrea-lu/ class=card-link><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path d="M224 256A128 128 0 10224 0a128 128 0 100 256zM448 512 384 304H64L0 512H448z"/></svg></span>Andrea Lu</a>
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<a href=/people/lance-noll/ class=card-link><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path d="M224 256A128 128 0 10224 0a128 128 0 100 256zM448 512 384 304H64L0 512H448z"/></svg></span>Lance Noll</a>
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<a href=/people/jianfa-bai/ class=card-link><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path d="M224 256A128 128 0 10224 0a128 128 0 100 256zM448 512 384 304H64L0 512H448z"/></svg></span>Jianfa Bai</a>
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<a href=/people/jamie-henningson/ class=card-link><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path d="M224 256A128 128 0 10224 0a128 128 0 100 256zM448 512 384 304H64L0 512H448z"/></svg></span>Jamie Henningson</a>
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<a href=/people/rachel-palinski/ class=card-link><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path d="M224 256A128 128 0 10224 0a128 128 0 100 256zM448 512 384 304H64L0 512H448z"/></svg></span>Rachel Palinski</a></small></h3><h4>April 27, 2022</h4><p>Enteric disease is the predominant cause of morbidity and mortality in young
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mammals including pigs. Viral species involved in porcine enteric disease
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complex (PEDC) include rotaviruses, coronaviruses, picornaviruses, astroviruses
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and pestiviruses among others. The virome of three groups of swine samples
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submitted to the Kansas State University Veterinary Diagnostic Laboratory for
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routine testing were assessed, namely, a Rotavirus A positive (RVA) group, a
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Rotavirus co-infection (RV) group and a Rotavirus Negative (RV Neg) group. All
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groups were designated by qRT-PCR results testing for Porcine Rotavirus A, B, C
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and H such that samples positive for RVA only went in the RVA group, samples
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positive for >1 rotavirus went in the RV group and samples negative for all were
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grouped in the RVNeg group. All of the animals had clinical enteric disease
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resulting in scours and swollen joints/lameness, enlarged heart and/or a cough.
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All samples were metagenomic sequenced and analyzed for viral species
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composition that identified 14 viral species and eight bacterial viruses/phages.
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Sapovirus and Escherichia coli phages were found at a high prevalence in RVA and
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RV samples but were found at low or no prevalence in the RV Neg samples.
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Picobirnavirus was identified at a high proportion and prevalence in RV Neg and
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RV samples but at a low prevalence in the RVA group. A sequence analysis of the
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possible host of Picobirnaviruses revealed fungi as the most likely host.
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Non-rotaviral diversity was highest in RVA samples followed by RV then RV Neg
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samples. Various sequences were extracted from the sample reads and a
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phylogenetic update was provided showing a high prevalence of G9 and P[23] RVA
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genotypes. These data are important for pathogen surveillance and control
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measures</p><a href=https://doi.org/10.1016/j.vetmic.2022.109447>https://doi.org/10.1016/j.vetmic.2022.109447</a>
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<iframe src=https://doi.org/10.1016/j.vetmic.2022.109447 style=width:100%;height:75vh></iframe></section></main></div><footer><img src=/graphics/brandedbull.min.svg height=95rem><p>© 2022 Thomas A. Christensen II<br>Licensed
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