53 lines
No EOL
6.6 KiB
HTML
53 lines
No EOL
6.6 KiB
HTML
<!doctype html><html class=no-js lang=en><head><meta charset=utf-8><meta http-equiv=x-ua-compatible content="ie=edge"><meta name=viewport content="width=device-width,initial-scale=1"><title>Metagenomic analysis of rumen populations in week-old calves as altered by maternal late gestational nutrition and mode of delivery - MillironX</title>
|
|
<link href=/styles/millironx.min.css rel=stylesheet></head><body><header><object data=/graphics/millironx.svg>
|
|
<img src=/graphics/millironx.svg alt="Milliron X"></object><h1 class=font-small-caps>Milliron X</h1></header><div class=row><aside><nav><a href=/><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 576 512"><path d="M511.8 287.6H576V240L288.4.0.0 240v47.6H64.1V512H224V352H352V512H512.8l-1-224.4z"/></svg></span>
|
|
Home
|
|
</a><a href=/contact/><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 576 512"><path d="M224 0H0V512H384V427l-1.7 1.7-120.1 17.2-11.7 1.9-11.6 1.9-5.3-10.5L222.1 416H209.9l-11.6 23.2-4.4 8.8H184h-8-11.9l-3.4-11.4L144 381l-16.7 55.6L123.9 448H112 80 64V416H80h20.1l20.6-68.6 3.4-11.4H136h16 11.9l3.4 11.4 15.4 51.4 3-5.9 4.4-8.8H2e2h32 9.9l4.4 8.8 7.2 14.5 13.5-94 117-117V160H224V0zm32 0V128H384L256 0zM505 119.8l-41.3 41.3 71 71L576 190.8l-71-71zm-63.9 63.9L299.9 325 288 407.9 370.9 396 512.1 254.7l-71-71z"/></svg></span>
|
|
Contact
|
|
</a><a href=/academia/><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path d="M0 144 256 0 512 144v48H0V144zM0 512V464l64-48V224h64V416h40V224h64V416h48V224h64V416h40V224h64V416l64 48v48H0zM256 144a32 32 0 100-64 32 32 0 100 64z"/></svg></span>
|
|
Academia
|
|
</a><a href=/videos/><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 576 512"><path d="M384 64H0V448H384V64zM576 448V64L416 174.9V337.1L576 448z"/></svg></span>
|
|
Videos
|
|
</a><a href=/websites/><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 512 512"><path d="M0 32H512V480H0V32zm160 72v48H448V104H160zm-32-8H64v64h64V96z"/></svg></span>
|
|
Websites</a></nav></aside><main><section><h5>Westion Section American Society of Animal Science Annual Meeting: Boise, Idaho</h5><h2>Metagenomic analysis of rumen populations in week-old calves as altered by maternal late gestational nutrition and mode of delivery</h2><h3><small><a href=/people/thomas-a.-christensen-ii/ class="card-link
|
|
bolder"><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path d="M224 256A128 128 0 10224 0a128 128 0 100 256zM448 512 384 304H64L0 512H448z"/></svg></span>
|
|
Thomas A. Christensen II</a>
|
|
<a href=/people/kathy-j.-austin/ class=card-link><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path d="M224 256A128 128 0 10224 0a128 128 0 100 256zM448 512 384 304H64L0 512H448z"/></svg></span>
|
|
Kathy J. Austin</a>
|
|
<a href=/people/kristi-m.-cammack/ class=card-link><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path d="M224 256A128 128 0 10224 0a128 128 0 100 256zM448 512 384 304H64L0 512H448z"/></svg></span>
|
|
Kristi M. Cammack</a>
|
|
<a href=/people/hannah-c.-cunningham-hollinger/ class=card-link><span class="fa-container fa-fw"><svg xmlns="http://www.w3.org/2000/svg" viewBox="0 0 448 512"><path d="M224 256A128 128 0 10224 0a128 128 0 100 256zM448 512 384 304H64L0 512H448z"/></svg></span>
|
|
Hannah C. Cunningham-Hollinger</a></small></h3><h4>June 12, 2019</h4><p>Early colonization of the rumen microbiome is critical to host health and long
|
|
term performance. Factors that influence early colonization include maternal
|
|
factors such as gestational nutrition and mode of delivery. Therefore, we
|
|
hypothesized that late gestational nutrition and mode of delivery would
|
|
influence the calf rumen microbiome. Our objectives were to determine if
|
|
nutrient restriction during late gestation alters the calf rumen microbiome and
|
|
determine if ruminal microbiome composition differs in calves born vaginally
|
|
versus caesarean. Late gestating Angus cows were randomly allocated to one of
|
|
three treatment groups: control (<strong>CON</strong>; n = 6), caesarean section (<strong>CS</strong>; n =
|
|
4), and nutrient restricted (<strong>NR</strong>; n = 5), where CON were fed DDGS and hay to
|
|
meet NRC requirements and calved naturally; CS were fed similarly to CON and
|
|
calves were born via caesarean section; and NR were fed at a level to reduce BCS
|
|
by 1.5-2.0 points over the last trimester compared to CON and calved naturally.
|
|
Rumen fluid was collected via oral lavage prior to partition from cows and at d
|
|
7 from calves. Microbial DNA was isolated from the rumen fluid and metagenomic
|
|
shotgun sequencing was performed using the Illumina HiSeq 2500 platform.
|
|
Sequence data were analyzed using Metaxa2 for taxonomic assignment followed by
|
|
QIIME1 and QIIME2 to determine differential abundance and alpha- and
|
|
beta-diversity differences. There were no significant differences in
|
|
alpha-diversity as measured by shannon index across treatment groups for cows
|
|
(<em>P</em> = 0.239), but there were significant differences for calves (<em>P</em> = 0.015).
|
|
Similarly, there were no significant differences in beta-diversity as measured
|
|
by the bray-curtis dissimilarity matrix for cows (<em>P</em> = 0.059), but there were
|
|
significant differences for calves (<em>P</em> = 0.007). Alpha-diversity differed (<em>P</em>
|
|
< 0.001) between cows and calves, with cows having increased species richness
|
|
compared to calves. Beta-diversity also differed (<em>P</em> = 0.001) between cows and
|
|
calves. At total of 410 taxa were differentially abundant (<em>P</em> < 0.01) between
|
|
cows and calves. These results suggest that the mature rumen microbiome of cows
|
|
is able to withstand changes in feed intake, however the calf microbiome is
|
|
susceptible to alteration by maternal factors. These data also suggest that
|
|
there may be opportunities to develop management strategies during late
|
|
gestation that influence calf health and performance long-term.</p><a href=/academia/metagenomics/metagenomics_analysis_of_rumen_populations.pdf>/academia/metagenomics/metagenomics_analysis_of_rumen_populations.pdf</a>
|
|
<iframe src=/academia/metagenomics/metagenomics_analysis_of_rumen_populations.pdf style=width:100%;height:75vh></iframe></section></main></div><footer><img src=/graphics/brandedbull.min.svg height=95rem><p>© 2019 Thomas A. Christensen II<br>Licensed
|
|
<a rel=license href=http://creativecommons.org/licenses/by/4.0/>CC-BY 4.0</a><br>Built with <a href=https://gohugo.io>Hugo</a> v0.126.1</p></footer><script data-goatcounter=https://millironx.goatcounter.com/count async src=//gc.zgo.at/count.js></script></body></html> |