Illumina

This script downloads all FASTQs associated with a specific Run ID from Illumina's Basespace.

Usage

To be run in Cygwin

dl-fastqs.sh

The script will prompt for a Run ID. Copy the Run ID from the URL in Illumina Basespace.

Note that Ctrl+V does not paste in either Cygwin or Windows terminals. Use right-click, instead.

This script requires Cygwin. The install process is complicated thanks to requiring native features of both Windows and Cygwin.

Download and install Cygwin
1. Select "Just Me" when asked who to install for
2. Find and add the latest version of the "git" packages
3. All other default options will work
Press Win+X, then I to open a new PowerShell window
Run wget 'https://api.bintray.com/content/basespace/BaseSpaceCLI-EarlyAccess-BIN/latest/\$latest/amd64-windows/bs.exe?bt_package=latest' -O $env:LOCALAPPDATA\Microsoft\WindowsApps\bs.exe
Close PowerShell

Open Cygwin Console and run

BS=$(cygpath "C:\Users\\$USER\AppData\Local\Microsoft\WindowsApps\bs.exe")
$BS auth

Copy the given link, navigate to it in a browser, and login to Illumina Basespace

In Cygwin console now run

git clone https://github.com/MillironX/wphl-bioinformatics.git
cp wphl-bioinformatics/illumina/dl-fastqs.sh ~
chmod +x dl-fastqs.sh

Right-click the Windows desktop, and click "New | Shortcut..."
Type C:\cygwin64\bin\bash.exe and click Next
Type Download Illumina Run or something else memorable and click "Finish"
Right-click the newly created shortcut and click Properties
In the Target textbox, add a space and --login dl-fastqs.sh to the existing text
Click OK