mirror of
https://github.com/MillironX/taxprofiler.git
synced 2024-11-26 02:09:54 +00:00
Update docs/output.md
Co-authored-by: James A. Fellows Yates <jfy133@gmail.com>
This commit is contained in:
parent
3c33ba66ca
commit
964771c5f2
1 changed files with 15 additions and 8 deletions
|
@ -209,20 +209,27 @@ You will only recieve the FASTQs and `*classifiedreads.txt` file if you supply `
|
||||||
|
|
||||||
### KrakenUniq
|
### KrakenUniq
|
||||||
|
|
||||||
|
[KrakenUniq](https://github.com/fbreitwieser/krakenuniq) (formerly KrakenHLL) is an extenson to the fast k-mer-based classification [Kraken](https://github.com/DerrickWood/kraken) with an efficient algorithm for additionally assessing the coverage of unique k-mers found in each species in a dataset.
|
||||||
|
|
||||||
<details markdown="1">
|
<details markdown="1">
|
||||||
<summary>Output files</summary>
|
<summary>Output files</summary>
|
||||||
|
|
||||||
- `krakenuniq`
|
- `krakenuniq/`
|
||||||
|
- <db_name>/
|
||||||
- `<sample_id>.classified.fastq.gz`
|
- `<sample_id>_<db_name>.classified.fastq.gz`: FASTQ file containing all reads that had a hit against a reference in the database for a given sample
|
||||||
- `<sample_id>.krakenuniq.classified.txt`
|
- `<sample_id>_<db_name>.unclassified.fastq.gz`: FASTQ file containing all reads that did not have a hit in the database for a given sample
|
||||||
- `<sample_id>.krakenuniq.report.txt`
|
- `<sample_id>_<db_name>.report.txt`: A Kraken2-style report that summarises the fraction abundance, taxonomic ID, number of Kmers, taxonomic path of all the hits, with an additional column for k-mer coverage, that allows for more accurate distinguishing between false-positive/true-postitive hits
|
||||||
- `<sample_id>.unclassified.fastq.gz`
|
- `<sample_id>_<db_name>.classifiedreads.txt`: A list of read IDs and the hits each read had against each database for a given sample
|
||||||
|
|
||||||
## interleaved?
|
|
||||||
|
|
||||||
</details>
|
</details>
|
||||||
|
|
||||||
|
The main taxonomic profiling file from KrakenUniq is the `*report.txt` file. This is an extension of the Kraken2 report with the additional k-mer coverage information that provides more information about the accuracy of hits.
|
||||||
|
|
||||||
|
> ⚠️ The output system of KrakenUniq can result in other `stdout` or `stderr` logging information being saved in the report file, therefore you must check your report files before downstream use!
|
||||||
|
|
||||||
|
You will only receive the FASTQs and `*classifiedreads.txt` file if you supply `--krakenuniq_save_reads` and/or `--krakenuniq_save_readclassification` parameters to the pipeline.
|
||||||
|
|
||||||
|
|
||||||
### Centrifuge
|
### Centrifuge
|
||||||
|
|
||||||
<details markdown="1">
|
<details markdown="1">
|
||||||
|
|
Loading…
Reference in a new issue