haplotyper-battle-royale/main.nf

#!/usr/bin/env nextflow

include { CLIQUESNV } from './modules/cliquesnv'
include { EFETCH } from './modules/efetch'
include { HAPLINK_HAPLOTYPES as HAPLINK_ML_HAPLOTYPES } from './modules/haplink/haplotypes'
include { HAPLINK_HAPLOTYPES as HAPLINK_RAW_HAPLOTYPES } from './modules/haplink/haplotypes'
include { HAPLINK_SEQUENCES } from './modules/haplink/sequences'
include { HAPLINK_VARIANTS } from './modules/haplink/variants'
include { MINIMAP2 } from './modules/minimap2'
include { NANOFILT } from './modules/nanofilt'
include { QUASIRECOMB } from './modules/quasirecomb'
include { SHORAH_AMPLICON } from './modules/shorah/amplicon'
include { SHORAH_SHOTGUN } from './modules/shorah/shotgun'
include { VIQUAS } from './modules/viquas'

workflow {

    Channel
        .fromPath("*.fastq.gz")
        .map { file -> tuple(file.simpleName, file) }
        .set { ch_input }

    EFETCH('NC_036618.1')
    EFETCH
        .out
        .set { ch_reference }

    NANOFILT( ch_input )
    NANOFILT
        .out
        .set { ch_reads }

    MINIMAP2( ch_reads, ch_reference )
    MINIMAP2
        .out
        .set { ch_alignments }

    CLIQUESNV(
        ch_alignments,
        'snv-pacbio'
    )

    HAPLINK_VARIANTS( ch_alignments, ch_reference )
    HAPLINK_VARIANTS
        .out
        .set { ch_variants }

    ch_alignments
        .join( ch_variants )
        .set { ch_haplotype_calling }


    HAPLINK_RAW_HAPLOTYPES(
        ch_haplotype_calling,
        ch_reference
    )
    HAPLINK_RAW_HAPLOTYPES
        .out
    .map{ [ it[0], 'HAPLINK_RAW_HAPLOTYPES', it[1] ] }
    .set{ ch_raw_haplotypes }

    HAPLINK_ML_HAPLOTYPES(
        ch_haplotype_calling,
        ch_reference
    )
    HAPLINK_ML_HAPLOTYPES
        .out
        .map{ [ it[0], 'HAPLINK_ML_HAPLOTYPES', it[1] ] }
        .set{ ch_ml_haplotypes }

    ch_raw_haplotypes
        .mix(ch_ml_haplotypes)
    .set{ ch_all_haplotypes }

    HAPLINK_SEQUENCES(
        ch_all_haplotypes,
        ch_reference
    )

    QUASIRECOMB( ch_alignments )

    SHORAH_AMPLICON(
        ch_alignments,
        ch_reference
    )

    SHORAH_SHOTGUN(
        ch_alignments,
        ch_reference
    )

    VIQUAS(
        ch_alignments,
        ch_reference
    )
}