mirror of
https://github.com/MillironX/nf-core_modules.git
synced 2024-11-11 04:33:10 +00:00
Merge branch 'antismashlite' of github.com:jasmezz/modules into antismashlite
This commit is contained in:
jasmezz
commit
d19e65d2a2
91 changed files with 1194 additions and 326 deletions
64
.github/ISSUE_TEMPLATE/bug_report.md
vendored
64
.github/ISSUE_TEMPLATE/bug_report.md
vendored
|
|
@ -1,64 +0,0 @@
|
|||
---
|
||||
name: Bug report
|
||||
about: Report something that is broken or incorrect
|
||||
title: "[BUG]"
|
||||
---
|
||||
|
||||
<!--
|
||||
# nf-core/module bug report
|
||||
|
||||
Hi there!
|
||||
|
||||
Thanks for telling us about a problem with the modules.
|
||||
Please delete this text and anything that's not relevant from the template below:
|
||||
-->
|
||||
|
||||
## Check Documentation
|
||||
|
||||
I have checked the following places for your error:
|
||||
|
||||
- [ ] [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)
|
||||
- [ ] [nf-core/module documentation](https://github.com/nf-core/modules/blob/master/README.md)
|
||||
|
||||
## Description of the bug
|
||||
|
||||
<!-- A clear and concise description of what the bug is. -->
|
||||
|
||||
## Steps to reproduce
|
||||
|
||||
Steps to reproduce the behaviour:
|
||||
|
||||
1. Command line: <!-- [e.g. `nextflow run ...`] -->
|
||||
2. See error: <!-- [Please provide your error message] -->
|
||||
|
||||
## Expected behaviour
|
||||
|
||||
<!-- A clear and concise description of what you expected to happen. -->
|
||||
|
||||
## Log files
|
||||
|
||||
Have you provided the following extra information/files:
|
||||
|
||||
- [ ] The command used to run the module
|
||||
- [ ] The `.nextflow.log` file <!-- this is a hidden file in the directory where you launched the module -->
|
||||
|
||||
## System
|
||||
|
||||
- Hardware: <!-- [e.g. HPC, Desktop, Cloud...] -->
|
||||
- Executor: <!-- [e.g. slurm, local, awsbatch...] -->
|
||||
- OS: <!-- [e.g. CentOS Linux, macOS, Linux Mint...] -->
|
||||
- Version <!-- [e.g. 7, 10.13.6, 18.3...] -->
|
||||
|
||||
## Nextflow Installation
|
||||
|
||||
- Version: <!-- [e.g. 19.10.0] -->
|
||||
|
||||
## Container engine
|
||||
|
||||
- Engine: <!-- [e.g. Conda, Docker, Singularity or Podman] -->
|
||||
- version: <!-- [e.g. 1.0.0] -->
|
||||
- Image tag: <!-- [e.g. nfcore/module:2.6] -->
|
||||
|
||||
## Additional context
|
||||
|
||||
<!-- Add any other context about the problem here. -->
|
||||
52
.github/ISSUE_TEMPLATE/bug_report.yml
vendored
Normal file
52
.github/ISSUE_TEMPLATE/bug_report.yml
vendored
Normal file
|
|
@ -0,0 +1,52 @@
|
|||
name: Bug report
|
||||
description: Report something that is broken or incorrect
|
||||
labels: bug
|
||||
body:
|
||||
- type: checkboxes
|
||||
attributes:
|
||||
label: Have you checked the docs?
|
||||
description: I have checked the following places for my error
|
||||
options:
|
||||
- label: "[nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting)"
|
||||
required: true
|
||||
- label: "[nf-core modules documentation](https://nf-co.re/docs/contributing/modules)"
|
||||
required: true
|
||||
|
||||
- type: textarea
|
||||
id: description
|
||||
attributes:
|
||||
label: Description of the bug
|
||||
description: A clear and concise description of what the bug is.
|
||||
validations:
|
||||
required: true
|
||||
|
||||
- type: textarea
|
||||
id: command_used
|
||||
attributes:
|
||||
label: Command used and terminal output
|
||||
description: Steps to reproduce the behaviour. Please paste the command you used to launch the pipeline and the output from your terminal.
|
||||
render: console
|
||||
placeholder: |
|
||||
$ nextflow run ...
|
||||
|
||||
Some output where something broke
|
||||
|
||||
- type: textarea
|
||||
id: files
|
||||
attributes:
|
||||
label: Relevant files
|
||||
description: |
|
||||
Please drag and drop the relevant files here. Create a `.zip` archive if the extension is not allowed.
|
||||
Your verbose log file `.nextflow.log` is often useful _(this is a hidden file in the directory where you launched the pipeline)_ as well as custom Nextflow configuration files.
|
||||
|
||||
- type: textarea
|
||||
id: system
|
||||
attributes:
|
||||
label: System information
|
||||
description: |
|
||||
* Nextflow version _(eg. 21.10.3)_
|
||||
* Hardware _(eg. HPC, Desktop, Cloud)_
|
||||
* Executor _(eg. slurm, local, awsbatch)_
|
||||
* Container engine and version: _(e.g. Docker 1.0.0, Singularity, Conda, Podman, Shifter or Charliecloud)_
|
||||
* OS and version: _(eg. CentOS Linux, macOS, Ubuntu 22.04)_
|
||||
* Image tag: <!-- [e.g. nfcore/cellranger:2.6] -->
|
||||
32
.github/ISSUE_TEMPLATE/feature_request.md
vendored
32
.github/ISSUE_TEMPLATE/feature_request.md
vendored
|
|
@ -1,32 +0,0 @@
|
|||
---
|
||||
name: Feature request
|
||||
about: Suggest an idea for nf-core/modules
|
||||
title: "[FEATURE]"
|
||||
---
|
||||
|
||||
<!--
|
||||
# nf-core/modules feature request
|
||||
|
||||
Hi there!
|
||||
|
||||
Thanks for suggesting a new feature for the modules!
|
||||
Please delete this text and anything that's not relevant from the template below:
|
||||
-->
|
||||
|
||||
## Is your feature request related to a problem? Please describe
|
||||
|
||||
<!-- A clear and concise description of what the problem is. -->
|
||||
|
||||
<!-- e.g. [I'm always frustrated when ...] -->
|
||||
|
||||
## Describe the solution you'd like
|
||||
|
||||
<!-- A clear and concise description of what you want to happen. -->
|
||||
|
||||
## Describe alternatives you've considered
|
||||
|
||||
<!-- A clear and concise description of any alternative solutions or features you've considered. -->
|
||||
|
||||
## Additional context
|
||||
|
||||
<!-- Add any other context about the feature request here. -->
|
||||
32
.github/ISSUE_TEMPLATE/feature_request.yml
vendored
Normal file
32
.github/ISSUE_TEMPLATE/feature_request.yml
vendored
Normal file
|
|
@ -0,0 +1,32 @@
|
|||
name: Feature request
|
||||
description: Suggest an idea for nf-core/modules
|
||||
labels: feature
|
||||
title: "[FEATURE]"
|
||||
body:
|
||||
- type: textarea
|
||||
id: description
|
||||
attributes:
|
||||
label: Is your feature request related to a problem? Please describe
|
||||
description: A clear and concise description of what the bug is.
|
||||
placeholder: |
|
||||
<!-- e.g. [I'm always frustrated when ...] -->
|
||||
validations:
|
||||
required: true
|
||||
|
||||
- type: textarea
|
||||
id: solution
|
||||
attributes:
|
||||
label: Describe the solution you'd like
|
||||
description: A clear and concise description of the solution you want to happen.
|
||||
|
||||
- type: textarea
|
||||
id: alternatives
|
||||
attributes:
|
||||
label: Describe alternatives you've considered
|
||||
description: A clear and concise description of any alternative solutions or features you've considered.
|
||||
|
||||
- type: textarea
|
||||
id: additional_context
|
||||
attributes:
|
||||
label: Additional context
|
||||
description: Add any other context about the feature request here.
|
||||
26
.github/ISSUE_TEMPLATE/new_module.md
vendored
26
.github/ISSUE_TEMPLATE/new_module.md
vendored
|
|
@ -1,26 +0,0 @@
|
|||
---
|
||||
name: New module
|
||||
about: Suggest a new module for nf-core/modules
|
||||
title: "new module: TOOL/SUBTOOL"
|
||||
label: new module
|
||||
---
|
||||
|
||||
<!--
|
||||
# nf-core/modules new module suggestion
|
||||
|
||||
Hi there!
|
||||
|
||||
Thanks for suggesting a new module for the modules!
|
||||
Please delete this text and anything that's not relevant from the template below:
|
||||
|
||||
Replace TOOL with the bioconda name for the tool in the following text, so that the link is functional.
|
||||
|
||||
Replace TOOL/SUBTOOL in the issue title so that it's understandable.
|
||||
-->
|
||||
|
||||
I think it would be good to have a module for [TOOL](https://bioconda.github.io/recipes/TOOL/README.html)
|
||||
|
||||
- [ ] This module does not exist yet with the [`nf-core modules list`](https://github.com/nf-core/tools#list-modules) command
|
||||
- [ ] There is no [open pull request](https://github.com/nf-core/modules/pulls) for this module
|
||||
- [ ] There is no [open issue](https://github.com/nf-core/modules/issues) for this module
|
||||
- [ ] If I'm planning to work on this module, I added myself to the `Assignees` to facilitate tracking who is working on the module
|
||||
36
.github/ISSUE_TEMPLATE/new_module.yml
vendored
Normal file
36
.github/ISSUE_TEMPLATE/new_module.yml
vendored
Normal file
|
|
@ -0,0 +1,36 @@
|
|||
name: New module
|
||||
description: Suggest a new module for nf-core/modules
|
||||
title: "new module: TOOL/SUBTOOL"
|
||||
labels: new module
|
||||
body:
|
||||
- type: checkboxes
|
||||
attributes:
|
||||
label: Is there an existing module for this?
|
||||
description: This module does not exist yet with the [`nf-core modules list`](https://github.com/nf-core/tools#list-modules) command
|
||||
options:
|
||||
- label: I have searched for the existing module
|
||||
required: true
|
||||
|
||||
- type: checkboxes
|
||||
attributes:
|
||||
label: Is there an open PR for this?
|
||||
description: There is no [open pull request](https://github.com/nf-core/modules/pulls) for this module
|
||||
options:
|
||||
- label: I have searched for existing PRs
|
||||
required: true
|
||||
|
||||
- type: checkboxes
|
||||
attributes:
|
||||
label: Is there an open issue for this?
|
||||
description: There is no [open issue](https://github.com/nf-core/modules/issues) for this module
|
||||
options:
|
||||
- label: I have searched for existing issues
|
||||
required: true
|
||||
|
||||
- type: checkboxes
|
||||
attributes:
|
||||
label: Are you going to work on this?
|
||||
description: If I'm planning to work on this module, I added myself to the `Assignees` to facilitate tracking who is working on the module
|
||||
options:
|
||||
- label: If I'm planning to work on this module, I added myself to the `Assignees` to facilitate tracking who is working on the module
|
||||
required: false
|
||||
|
|
@ -7,8 +7,9 @@ process ANTISMASH_ANTISMASHLITEDOWNLOADDATABASES {
|
|||
'quay.io/biocontainers/antismash-lite:6.0.1--pyhdfd78af_1' }"
|
||||
|
||||
/*
|
||||
These files are normally downloaded by download-antismash-databases itself, and must be retrieved for input by manually running the command with conda or a standalone installation of antiSMASH. Therefore we do not recommend using this module for production pipelines, but rather require users to specify their own local copy of the antiSMASH database in pipelines. This is solely for use for CI tests of the nf-core/module version of antiSMASH.
|
||||
These files are normally downloaded/created by download-antismash-databases itself, and must be retrieved for input by manually running the command with conda or a standalone installation of antiSMASH. Therefore we do not recommend using this module for production pipelines, but rather require users to specify their own local copy of the antiSMASH database in pipelines. This is solely for use for CI tests of the nf-core/module version of antiSMASH.
|
||||
Reason: Upon execution, the tool checks if certain database files are present within the container and if not, it tries to create them in /usr/local/bin, for which only root user has write permissions. Mounting those database files with this module prevents the tool from trying to create them.
|
||||
These files are also emitted as output channels in this module to enable the antismash-lite module to use them as mount volumes to the docker/singularity containers.
|
||||
*/
|
||||
|
||||
containerOptions {
|
||||
|
|
@ -26,6 +27,9 @@ process ANTISMASH_ANTISMASHLITEDOWNLOADDATABASES {
|
|||
|
||||
output:
|
||||
path("antismash_db") , emit: database
|
||||
path("css"), emit: css_dir
|
||||
path("detection"), emit: detection_dir
|
||||
path("modules"), emit: modules_dir
|
||||
path "versions.yml", emit: versions
|
||||
|
||||
when:
|
||||
|
|
@ -40,7 +44,7 @@ process ANTISMASH_ANTISMASHLITEDOWNLOADDATABASES {
|
|||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
antismash: \$(antismash --version | sed 's/antiSMASH //')
|
||||
antismash-lite: \$(antismash --version | sed 's/antiSMASH //')
|
||||
END_VERSIONS
|
||||
"""
|
||||
}
|
||||
|
|
|
|||
|
|
@ -27,17 +27,17 @@ input:
|
|||
- database_css:
|
||||
type: directory
|
||||
description: |
|
||||
antismash/outputs/html/css folder which is being created during the antiSMASH database downloading step. These files are normally downloaded by download-antismash-databases itself, and must be retrieved by the use by manually running the command with conda or a standalone installation of antiSMASH. Therefore we do not recommend using this module for production pipelines, but rather require users to specify their own local copy of the antiSMASH database in pipelines.
|
||||
antismash/outputs/html/css folder which is being created during the antiSMASH database downloading step. These files are normally downloaded by download-antismash-databases itself, and must be retrieved by the user by manually running the command with conda or a standalone installation of antiSMASH. Therefore we do not recommend using this module for production pipelines, but rather require users to specify their own local copy of the antiSMASH database in pipelines.
|
||||
pattern: "css"
|
||||
- database_detection:
|
||||
type: directory
|
||||
description: |
|
||||
antismash/detection folder which is being created during the antiSMASH database downloading step. These files are normally downloaded by download-antismash-databases itself, and must be retrieved by the use by manually running the command with conda or a standalone installation of antiSMASH. Therefore we do not recommend using this module for production pipelines, but rather require users to specify their own local copy of the antiSMASH database in pipelines.
|
||||
antismash/detection folder which is being created during the antiSMASH database downloading step. These files are normally downloaded by download-antismash-databases itself, and must be retrieved by the user by manually running the command with conda or a standalone installation of antiSMASH. Therefore we do not recommend using this module for production pipelines, but rather require users to specify their own local copy of the antiSMASH database in pipelines.
|
||||
pattern: "detection"
|
||||
- database_modules:
|
||||
type: directory
|
||||
description: |
|
||||
antismash/modules folder which is being created during the antiSMASH database downloading step. These files are normally downloaded by download-antismash-databases itself, and must be retrieved by the use by manually running the command with conda or a standalone installation of antiSMASH. Therefore we do not recommend using this module for production pipelines, but rather require users to specify their own local copy of the antiSMASH database in pipelines.
|
||||
antismash/modules folder which is being created during the antiSMASH database downloading step. These files are normally downloaded by download-antismash-databases itself, and must be retrieved by the user by manually running the command with conda or a standalone installation of antiSMASH. Therefore we do not recommend using this module for production pipelines, but rather require users to specify their own local copy of the antiSMASH database in pipelines.
|
||||
pattern: "modules"
|
||||
|
||||
output:
|
||||
|
|
@ -50,6 +50,21 @@ output:
|
|||
type: directory
|
||||
description: Download directory for antiSMASH databases
|
||||
pattern: "antismash_db"
|
||||
- css_dir:
|
||||
type: directory
|
||||
description: |
|
||||
antismash/outputs/html/css folder which is being created during the antiSMASH database downloading step. These files are normally downloaded by download-antismash-databases itself, and must be retrieved by the user by manually running the command with conda or a standalone installation of antiSMASH. Therefore we do not recommend using this module for production pipelines, but rather require users to specify their own local copy of the antiSMASH database in pipelines.
|
||||
pattern: "css"
|
||||
- detection_dir:
|
||||
type: directory
|
||||
description: |
|
||||
antismash/detection folder which is being created during the antiSMASH database downloading step. These files are normally downloaded by download-antismash-databases itself, and must be retrieved by the user by manually running the command with conda or a standalone installation of antiSMASH. Therefore we do not recommend using this module for production pipelines, but rather require users to specify their own local copy of the antiSMASH database in pipelines.
|
||||
pattern: "detection"
|
||||
- modules_dir:
|
||||
type: directory
|
||||
description: |
|
||||
antismash/modules folder which is being created during the antiSMASH database downloading step. These files are normally downloaded by download-antismash-databases itself, and must be retrieved by the user by manually running the command with conda or a standalone installation of antiSMASH. Therefore we do not recommend using this module for production pipelines, but rather require users to specify their own local copy of the antiSMASH database in pipelines.
|
||||
pattern: "modules"
|
||||
|
||||
authors:
|
||||
- "@jasmezz"
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process BAMTOOLS_SPLIT {
|
|||
tag "$meta.id"
|
||||
label 'process_low'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::bamtools=2.5.1" : null)
|
||||
conda (params.enable_conda ? "bioconda::bamtools=2.5.2" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/bamtools:2.5.1--h9a82719_9' :
|
||||
'quay.io/biocontainers/bamtools:2.5.1--h9a82719_9' }"
|
||||
'https://depot.galaxyproject.org/singularity/bamtools:2.5.2--hd03093a_0' :
|
||||
'quay.io/biocontainers/bamtools:2.5.2--hd03093a_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bam)
|
||||
|
|
@ -20,11 +20,15 @@ process BAMTOOLS_SPLIT {
|
|||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
def input_list = bam.collect{"-in $it"}.join(' ')
|
||||
"""
|
||||
bamtools \\
|
||||
split \\
|
||||
-in $bam \\
|
||||
$args
|
||||
merge \\
|
||||
$input_list \\
|
||||
| bamtools \\
|
||||
split \\
|
||||
-stub $prefix \\
|
||||
$args
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
|||
|
|
@ -23,7 +23,7 @@ input:
|
|||
e.g. [ id:'test', single_end:false ]
|
||||
- bam:
|
||||
type: file
|
||||
description: A BAM file to split
|
||||
description: A list of one or more BAM files to merge and then split
|
||||
pattern: "*.bam"
|
||||
|
||||
output:
|
||||
|
|
@ -43,3 +43,4 @@ output:
|
|||
|
||||
authors:
|
||||
- "@sguizard"
|
||||
- "@matthdsm"
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process CUSTOM_GETCHROMSIZES {
|
|||
tag "$fasta"
|
||||
label 'process_low'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::samtools=1.15" : null)
|
||||
conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/samtools:1.15--h1170115_1' :
|
||||
'quay.io/biocontainers/samtools:1.15--h1170115_1' }"
|
||||
'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
|
||||
'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
|
||||
|
||||
input:
|
||||
path fasta
|
||||
|
|
|
|||
|
|
@ -2,20 +2,26 @@ process DIAMOND_BLASTP {
|
|||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
// Dimaond is limited to v2.0.9 because there is not a
|
||||
// singularity version higher than this at the current time.
|
||||
conda (params.enable_conda ? "bioconda::diamond=2.0.9" : null)
|
||||
conda (params.enable_conda ? "bioconda::diamond=2.0.15" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/diamond:2.0.9--hdcc8f71_0' :
|
||||
'quay.io/biocontainers/diamond:2.0.9--hdcc8f71_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/diamond:2.0.15--hb97b32f_0' :
|
||||
'quay.io/biocontainers/diamond:2.0.15--hb97b32f_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(fasta)
|
||||
path db
|
||||
path db
|
||||
val out_ext
|
||||
val blast_columns
|
||||
|
||||
output:
|
||||
tuple val(meta), path('*.txt'), emit: txt
|
||||
path "versions.yml" , emit: versions
|
||||
tuple val(meta), path('*.blast'), optional: true, emit: blast
|
||||
tuple val(meta), path('*.xml') , optional: true, emit: xml
|
||||
tuple val(meta), path('*.txt') , optional: true, emit: txt
|
||||
tuple val(meta), path('*.daa') , optional: true, emit: daa
|
||||
tuple val(meta), path('*.sam') , optional: true, emit: sam
|
||||
tuple val(meta), path('*.tsv') , optional: true, emit: tsv
|
||||
tuple val(meta), path('*.paf') , optional: true, emit: paf
|
||||
path "versions.yml" , emit: versions
|
||||
|
||||
when:
|
||||
task.ext.when == null || task.ext.when
|
||||
|
|
@ -23,6 +29,21 @@ process DIAMOND_BLASTP {
|
|||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
def columns = blast_columns ? "${blast_columns}" : ''
|
||||
switch ( out_ext ) {
|
||||
case "blast": outfmt = 0; break
|
||||
case "xml": outfmt = 5; break
|
||||
case "txt": outfmt = 6; break
|
||||
case "daa": outfmt = 100; break
|
||||
case "sam": outfmt = 101; break
|
||||
case "tsv": outfmt = 102; break
|
||||
case "paf": outfmt = 103; break
|
||||
default:
|
||||
outfmt = '6';
|
||||
out_ext = 'txt';
|
||||
log.warn("Unknown output file format provided (${out_ext}): selecting DIAMOND default of tabular BLAST output (txt)");
|
||||
break
|
||||
}
|
||||
"""
|
||||
DB=`find -L ./ -name "*.dmnd" | sed 's/.dmnd//'`
|
||||
|
||||
|
|
@ -31,8 +52,9 @@ process DIAMOND_BLASTP {
|
|||
--threads $task.cpus \\
|
||||
--db \$DB \\
|
||||
--query $fasta \\
|
||||
--outfmt ${outfmt} ${columns} \\
|
||||
$args \\
|
||||
--out ${prefix}.txt
|
||||
--out ${prefix}.${out_ext}
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
|||
|
|
@ -28,12 +28,50 @@ input:
|
|||
type: directory
|
||||
description: Directory containing the protein blast database
|
||||
pattern: "*"
|
||||
- out_ext:
|
||||
type: string
|
||||
description: |
|
||||
Specify the type of output file to be generated. `blast` corresponds to
|
||||
BLAST pairwise format. `xml` corresponds to BLAST xml format.
|
||||
`txt` corresponds to to BLAST tabular format. `tsv` corresponds to
|
||||
taxonomic classification format.
|
||||
pattern: "blast|xml|txt|daa|sam|tsv|paf"
|
||||
- blast_columns:
|
||||
type: string
|
||||
description: |
|
||||
Optional space separated list of DIAMOND tabular BLAST output keywords
|
||||
used for in conjunction with the 'txt' out_ext option (--outfmt 6). See
|
||||
DIAMOND documnetation for more information.
|
||||
|
||||
output:
|
||||
- txt:
|
||||
- blast:
|
||||
type: file
|
||||
description: File containing blastp hits
|
||||
pattern: "*.{blastp.txt}"
|
||||
pattern: "*.{blast}"
|
||||
- xml:
|
||||
type: file
|
||||
description: File containing blastp hits
|
||||
pattern: "*.{xml}"
|
||||
- txt:
|
||||
type: file
|
||||
description: File containing hits in tabular BLAST format.
|
||||
pattern: "*.{txt}"
|
||||
- daa:
|
||||
type: file
|
||||
description: File containing hits DAA format
|
||||
pattern: "*.{daa}"
|
||||
- sam:
|
||||
type: file
|
||||
description: File containing aligned reads in SAM format
|
||||
pattern: "*.{sam}"
|
||||
- tsv:
|
||||
type: file
|
||||
description: Tab separated file containing taxonomic classification of hits
|
||||
pattern: "*.{tsv}"
|
||||
- paf:
|
||||
type: file
|
||||
description: File containing aligned reads in pairwise mapping format format
|
||||
pattern: "*.{paf}"
|
||||
- versions:
|
||||
type: file
|
||||
description: File containing software versions
|
||||
|
|
@ -41,3 +79,4 @@ output:
|
|||
|
||||
authors:
|
||||
- "@spficklin"
|
||||
- "@jfy133"
|
||||
|
|
|
|||
|
|
@ -2,20 +2,26 @@ process DIAMOND_BLASTX {
|
|||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
// Dimaond is limited to v2.0.9 because there is not a
|
||||
// singularity version higher than this at the current time.
|
||||
conda (params.enable_conda ? "bioconda::diamond=2.0.9" : null)
|
||||
conda (params.enable_conda ? "bioconda::diamond=2.0.15" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/diamond:2.0.9--hdcc8f71_0' :
|
||||
'quay.io/biocontainers/diamond:2.0.9--hdcc8f71_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/diamond:2.0.15--hb97b32f_0' :
|
||||
'quay.io/biocontainers/diamond:2.0.15--hb97b32f_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(fasta)
|
||||
path db
|
||||
path db
|
||||
val out_ext
|
||||
val blast_columns
|
||||
|
||||
output:
|
||||
tuple val(meta), path('*.txt'), emit: txt
|
||||
path "versions.yml" , emit: versions
|
||||
tuple val(meta), path('*.blast'), optional: true, emit: blast
|
||||
tuple val(meta), path('*.xml') , optional: true, emit: xml
|
||||
tuple val(meta), path('*.txt') , optional: true, emit: txt
|
||||
tuple val(meta), path('*.daa') , optional: true, emit: daa
|
||||
tuple val(meta), path('*.sam') , optional: true, emit: sam
|
||||
tuple val(meta), path('*.tsv') , optional: true, emit: tsv
|
||||
tuple val(meta), path('*.paf') , optional: true, emit: paf
|
||||
path "versions.yml" , emit: versions
|
||||
|
||||
when:
|
||||
task.ext.when == null || task.ext.when
|
||||
|
|
@ -23,6 +29,21 @@ process DIAMOND_BLASTX {
|
|||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
def columns = blast_columns ? "${blast_columns}" : ''
|
||||
switch ( out_ext ) {
|
||||
case "blast": outfmt = 0; break
|
||||
case "xml": outfmt = 5; break
|
||||
case "txt": outfmt = 6; break
|
||||
case "daa": outfmt = 100; break
|
||||
case "sam": outfmt = 101; break
|
||||
case "tsv": outfmt = 102; break
|
||||
case "paf": outfmt = 103; break
|
||||
default:
|
||||
outfmt = '6';
|
||||
out_ext = 'txt';
|
||||
log.warn("Unknown output file format provided (${out_ext}): selecting DIAMOND default of tabular BLAST output (txt)");
|
||||
break
|
||||
}
|
||||
"""
|
||||
DB=`find -L ./ -name "*.dmnd" | sed 's/.dmnd//'`
|
||||
|
||||
|
|
@ -31,8 +52,9 @@ process DIAMOND_BLASTX {
|
|||
--threads $task.cpus \\
|
||||
--db \$DB \\
|
||||
--query $fasta \\
|
||||
--outfmt ${outfmt} ${columns} \\
|
||||
$args \\
|
||||
--out ${prefix}.txt
|
||||
--out ${prefix}.${out_ext}
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
|||
|
|
@ -28,12 +28,44 @@ input:
|
|||
type: directory
|
||||
description: Directory containing the nucelotide blast database
|
||||
pattern: "*"
|
||||
- out_ext:
|
||||
type: string
|
||||
description: |
|
||||
Specify the type of output file to be generated. `blast` corresponds to
|
||||
BLAST pairwise format. `xml` corresponds to BLAST xml format.
|
||||
`txt` corresponds to to BLAST tabular format. `tsv` corresponds to
|
||||
taxonomic classification format.
|
||||
pattern: "blast|xml|txt|daa|sam|tsv|paf"
|
||||
|
||||
output:
|
||||
- blast:
|
||||
type: file
|
||||
description: File containing blastp hits
|
||||
pattern: "*.{blast}"
|
||||
- xml:
|
||||
type: file
|
||||
description: File containing blastp hits
|
||||
pattern: "*.{xml}"
|
||||
- txt:
|
||||
type: file
|
||||
description: File containing blastx hits
|
||||
pattern: "*.{blastx.txt}"
|
||||
description: File containing hits in tabular BLAST format.
|
||||
pattern: "*.{txt}"
|
||||
- daa:
|
||||
type: file
|
||||
description: File containing hits DAA format
|
||||
pattern: "*.{daa}"
|
||||
- sam:
|
||||
type: file
|
||||
description: File containing aligned reads in SAM format
|
||||
pattern: "*.{sam}"
|
||||
- tsv:
|
||||
type: file
|
||||
description: Tab separated file containing taxonomic classification of hits
|
||||
pattern: "*.{tsv}"
|
||||
- paf:
|
||||
type: file
|
||||
description: File containing aligned reads in pairwise mapping format format
|
||||
pattern: "*.{paf}"
|
||||
- versions:
|
||||
type: file
|
||||
description: File containing software versions
|
||||
|
|
@ -41,3 +73,4 @@ output:
|
|||
|
||||
authors:
|
||||
- "@spficklin"
|
||||
- "@jfy133"
|
||||
|
|
|
|||
|
|
@ -2,12 +2,10 @@ process DIAMOND_MAKEDB {
|
|||
tag "$fasta"
|
||||
label 'process_medium'
|
||||
|
||||
// Dimaond is limited to v2.0.9 because there is not a
|
||||
// singularity version higher than this at the current time.
|
||||
conda (params.enable_conda ? 'bioconda::diamond=2.0.9' : null)
|
||||
conda (params.enable_conda ? "bioconda::diamond=2.0.15" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/diamond:2.0.9--hdcc8f71_0' :
|
||||
'quay.io/biocontainers/diamond:2.0.9--hdcc8f71_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/diamond:2.0.15--hb97b32f_0' :
|
||||
'quay.io/biocontainers/diamond:2.0.15--hb97b32f_0' }"
|
||||
|
||||
input:
|
||||
path fasta
|
||||
|
|
|
|||
43
modules/elprep/merge/main.nf
Normal file
43
modules/elprep/merge/main.nf
Normal file
|
|
@ -0,0 +1,43 @@
|
|||
process ELPREP_MERGE {
|
||||
tag "$meta.id"
|
||||
label 'process_low'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::elprep=5.1.2" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/elprep:5.1.2--he881be0_0':
|
||||
'quay.io/biocontainers/elprep:5.1.2--he881be0_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bam)
|
||||
|
||||
output:
|
||||
tuple val(meta), path("output/**.{bam,sam}") , emit: bam
|
||||
path "versions.yml" , emit: versions
|
||||
|
||||
when:
|
||||
task.ext.when == null || task.ext.when
|
||||
|
||||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
def suffix = args.contains("--output-type sam") ? "sam" : "bam"
|
||||
def single_end = meta.single_end ? " --single-end" : ""
|
||||
|
||||
"""
|
||||
# create directory and move all input so elprep can find and merge them before splitting
|
||||
mkdir input
|
||||
mv ${bam} input/
|
||||
|
||||
elprep merge \\
|
||||
input/ \\
|
||||
output/${prefix}.${suffix} \\
|
||||
$args \\
|
||||
${single_end} \\
|
||||
--nr-of-threads $task.cpus
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
elprep: \$(elprep 2>&1 | head -n2 | tail -n1 |sed 's/^.*version //;s/ compiled.*\$//')
|
||||
END_VERSIONS
|
||||
"""
|
||||
}
|
||||
44
modules/elprep/merge/meta.yml
Normal file
44
modules/elprep/merge/meta.yml
Normal file
|
|
@ -0,0 +1,44 @@
|
|||
name: "elprep_merge"
|
||||
description: Merge split bam/sam chunks in one file
|
||||
keywords:
|
||||
- bam
|
||||
- sam
|
||||
- merge
|
||||
tools:
|
||||
- "elprep":
|
||||
description: "elPrep is a high-performance tool for preparing .sam/.bam files for variant calling in sequencing pipelines. It can be used as a drop-in replacement for SAMtools/Picard/GATK4."
|
||||
homepage: "https://github.com/ExaScience/elprep"
|
||||
documentation: "https://github.com/ExaScience/elprep"
|
||||
tool_dev_url: "https://github.com/ExaScience/elprep"
|
||||
doi: "10.1371/journal.pone.0244471"
|
||||
licence: "['AGPL v3']"
|
||||
|
||||
input:
|
||||
- meta:
|
||||
type: map
|
||||
description: |
|
||||
Groovy Map containing sample information
|
||||
e.g. [ id:'test', single_end:false ]
|
||||
- bam:
|
||||
type: file
|
||||
description: List of BAM/SAM chunks to merge
|
||||
pattern: "*.{bam,sam}"
|
||||
|
||||
output:
|
||||
- meta:
|
||||
type: map
|
||||
description: |
|
||||
Groovy Map containing sample information
|
||||
e.g. [ id:'test', single_end:false ]
|
||||
#
|
||||
- versions:
|
||||
type: file
|
||||
description: File containing software versions
|
||||
pattern: "versions.yml"
|
||||
- bam:
|
||||
type: file
|
||||
description: Merged BAM/SAM file
|
||||
pattern: "*.{bam,sam}"
|
||||
|
||||
authors:
|
||||
- "@matthdsm"
|
||||
|
|
@ -17,7 +17,7 @@ process GATK4_HAPLOTYPECALLER {
|
|||
|
||||
output:
|
||||
tuple val(meta), path("*.vcf.gz"), emit: vcf
|
||||
tuple val(meta), path("*.tbi") , emit: tbi
|
||||
tuple val(meta), path("*.tbi") , optional:true, emit: tbi
|
||||
path "versions.yml" , emit: versions
|
||||
|
||||
when:
|
||||
|
|
|
|||
|
|
@ -12,7 +12,7 @@ process GATK4_MARKDUPLICATES {
|
|||
|
||||
output:
|
||||
tuple val(meta), path("*.bam") , emit: bam
|
||||
tuple val(meta), path("*.bai") , emit: bai
|
||||
tuple val(meta), path("*.bai") , optional:true, emit: bai
|
||||
tuple val(meta), path("*.metrics"), emit: metrics
|
||||
path "versions.yml" , emit: versions
|
||||
|
||||
|
|
|
|||
|
|
@ -8,7 +8,7 @@ process GATK4_SPLITNCIGARREADS {
|
|||
'quay.io/biocontainers/gatk4:4.2.5.0--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bam)
|
||||
tuple val(meta), path(bam), path(bai), path(intervals)
|
||||
path fasta
|
||||
path fai
|
||||
path dict
|
||||
|
|
@ -23,6 +23,7 @@ process GATK4_SPLITNCIGARREADS {
|
|||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
def interval_command = intervals ? "--intervals $intervals" : ""
|
||||
|
||||
def avail_mem = 3
|
||||
if (!task.memory) {
|
||||
|
|
@ -35,6 +36,7 @@ process GATK4_SPLITNCIGARREADS {
|
|||
--input $bam \\
|
||||
--output ${prefix}.bam \\
|
||||
--reference $fasta \\
|
||||
$interval_command \\
|
||||
--tmp-dir . \\
|
||||
$args
|
||||
|
||||
|
|
|
|||
|
|
@ -23,6 +23,13 @@ input:
|
|||
type: list
|
||||
description: BAM/SAM/CRAM file containing reads
|
||||
pattern: "*.{bam,sam,cram}"
|
||||
- bai:
|
||||
type: list
|
||||
description: BAI/SAI/CRAI index file (optional)
|
||||
pattern: "*.{bai,sai,crai}"
|
||||
- intervals:
|
||||
type: file
|
||||
description: Bed file with the genomic regions included in the library (optional)
|
||||
- fasta:
|
||||
type: file
|
||||
description: The reference fasta file
|
||||
|
|
|
|||
34
modules/krona/ktimporttext/main.nf
Normal file
34
modules/krona/ktimporttext/main.nf
Normal file
|
|
@ -0,0 +1,34 @@
|
|||
process KRONA_KTIMPORTTEXT {
|
||||
tag "$meta.id"
|
||||
label 'process_low'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::krona=2.8.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/krona:2.8.1--pl5321hdfd78af_1':
|
||||
'quay.io/biocontainers/krona:2.8.1--pl5321hdfd78af_1' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(report)
|
||||
|
||||
output:
|
||||
tuple val(meta), path ('*.html'), emit: html
|
||||
path "versions.yml" , emit: versions
|
||||
|
||||
when:
|
||||
task.ext.when == null || task.ext.when
|
||||
|
||||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
"""
|
||||
ktImportText \\
|
||||
$args \\
|
||||
-o ${prefix}.html \\
|
||||
$report
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
krona: \$( echo \$(ktImportText 2>&1) | sed 's/^.*KronaTools //g; s/- ktImportText.*\$//g')
|
||||
END_VERSIONS
|
||||
"""
|
||||
}
|
||||
47
modules/krona/ktimporttext/meta.yml
Normal file
47
modules/krona/ktimporttext/meta.yml
Normal file
|
|
@ -0,0 +1,47 @@
|
|||
name: "krona_ktimporttext"
|
||||
description: Creates a Krona chart from text files listing quantities and lineages.
|
||||
keywords:
|
||||
- plot
|
||||
- taxonomy
|
||||
- interactive
|
||||
- html
|
||||
- visualisation
|
||||
- krona chart
|
||||
- metagenomics
|
||||
tools:
|
||||
- krona:
|
||||
description: Krona Tools is a set of scripts to create Krona charts from several Bioinformatics tools as well as from text and XML files.
|
||||
homepage: https://github.com/marbl/Krona/wiki/KronaTools
|
||||
documentation: http://manpages.ubuntu.com/manpages/impish/man1/ktImportTaxonomy.1.html
|
||||
tool_dev_url: https://github.com/marbl/Krona
|
||||
doi: 10.1186/1471-2105-12-385
|
||||
licence: https://raw.githubusercontent.com/marbl/Krona/master/KronaTools/LICENSE.txt
|
||||
|
||||
input:
|
||||
- meta:
|
||||
type: map
|
||||
description: |
|
||||
Groovy Map containing sample information
|
||||
e.g. [ id:'test']
|
||||
- report:
|
||||
type: file
|
||||
description: "Tab-delimited text file. Each line should be a number followed by a list of wedges to contribute to (starting from the highest level). If no wedges are listed (and just a quantity is given), it will contribute to the top level. If the same lineage is listed more than once, the values will be added. Quantities can be omitted if -q is specified. Lines beginning with '#' will be ignored."
|
||||
pattern: "*.{txt}"
|
||||
|
||||
output:
|
||||
- meta:
|
||||
type: map
|
||||
description: |
|
||||
Groovy Map containing sample information
|
||||
e.g. [ id:'test' ]
|
||||
- versions:
|
||||
type: file
|
||||
description: File containing software versions
|
||||
pattern: "versions.yml"
|
||||
- html:
|
||||
type: file
|
||||
description: A html file containing an interactive krona plot.
|
||||
pattern: "*.{html}"
|
||||
|
||||
authors:
|
||||
- "@jianhong"
|
||||
|
|
@ -2,18 +2,22 @@ process MINIMAP2_ALIGN {
|
|||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
conda (params.enable_conda ? 'bioconda::minimap2=2.21' : null)
|
||||
conda (params.enable_conda ? 'bioconda::minimap2=2.21 bioconda::samtools=1.12' : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/minimap2:2.21--h5bf99c6_0' :
|
||||
'quay.io/biocontainers/minimap2:2.21--h5bf99c6_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' :
|
||||
'quay.io/biocontainers/mulled-v2-66534bcbb7031a148b13e2ad42583020b9cd25c4:1679e915ddb9d6b4abda91880c4b48857d471bd8-0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(reads)
|
||||
path reference
|
||||
val bam_format
|
||||
val cigar_paf_format
|
||||
val cigar_bam
|
||||
|
||||
output:
|
||||
tuple val(meta), path("*.paf"), emit: paf
|
||||
path "versions.yml" , emit: versions
|
||||
tuple val(meta), path("*.paf"), optional: true, emit: paf
|
||||
tuple val(meta), path("*.bam"), optional: true, emit: bam
|
||||
path "versions.yml" , emit: versions
|
||||
|
||||
when:
|
||||
task.ext.when == null || task.ext.when
|
||||
|
|
@ -22,13 +26,19 @@ process MINIMAP2_ALIGN {
|
|||
def args = task.ext.args ?: ''
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
def input_reads = meta.single_end ? "$reads" : "${reads[0]} ${reads[1]}"
|
||||
def bam_output = bam_format ? "-a | samtools sort | samtools view -@ ${task.cpus} -b -h -o ${prefix}.bam" : "-o ${prefix}.paf"
|
||||
def cigar_paf = cigar_paf_format && !bam_format ? "-c" : ''
|
||||
def set_cigar_bam = cigar_bam && bam_format ? "-L" : ''
|
||||
"""
|
||||
minimap2 \\
|
||||
$args \\
|
||||
-t $task.cpus \\
|
||||
$reference \\
|
||||
$input_reads \\
|
||||
> ${prefix}.paf
|
||||
$cigar_paf \\
|
||||
$set_cigar_bam \\
|
||||
$bam_output
|
||||
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
|||
|
|
@ -29,6 +29,17 @@ input:
|
|||
type: file
|
||||
description: |
|
||||
Reference database in FASTA format.
|
||||
- bam_format:
|
||||
type: boolean
|
||||
description: Specify that output should be in BAM format
|
||||
- cigar_paf_format:
|
||||
type: boolean
|
||||
description: Specify that output CIGAR should be in PAF format
|
||||
- cigar_bam:
|
||||
type: boolean
|
||||
description: |
|
||||
Write CIGAR with >65535 ops at the CG tag. This is recommended when
|
||||
doing XYZ (https://github.com/lh3/minimap2#working-with-65535-cigar-operations)
|
||||
output:
|
||||
- meta:
|
||||
type: map
|
||||
|
|
@ -39,9 +50,16 @@ output:
|
|||
type: file
|
||||
description: Alignment in PAF format
|
||||
pattern: "*.paf"
|
||||
- bam:
|
||||
type: file
|
||||
description: Alignment in BAM format
|
||||
pattern: "*.bam"
|
||||
- versions:
|
||||
type: file
|
||||
description: File containing software versions
|
||||
pattern: "versions.yml"
|
||||
authors:
|
||||
- "@heuermh"
|
||||
- "@sofstam"
|
||||
- "@sateeshperi"
|
||||
- "@jfy133"
|
||||
|
|
|
|||
|
|
@ -22,11 +22,12 @@ process PHANTOMPEAKQUALTOOLS {
|
|||
task.ext.when == null || task.ext.when
|
||||
|
||||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def args = task.ext.args ?: ''
|
||||
def args2 = task.ext.args2 ?: ''
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
"""
|
||||
RUN_SPP=`which run_spp.R`
|
||||
Rscript $args -e "library(caTools); source(\\"\$RUN_SPP\\")" -c="$bam" -savp="${prefix}.spp.pdf" -savd="${prefix}.spp.Rdata" -out="${prefix}.spp.out"
|
||||
Rscript $args -e "library(caTools); source(\\"\$RUN_SPP\\")" -c="$bam" -savp="${prefix}.spp.pdf" -savd="${prefix}.spp.Rdata" -out="${prefix}.spp.out" $args2
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_ADDORREPLACEREADGROUPS {
|
|||
tag "$meta.id"
|
||||
label 'process_low'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.9" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.9--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.9--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bam)
|
||||
|
|
@ -38,12 +38,12 @@ process PICARD_ADDORREPLACEREADGROUPS {
|
|||
-Xmx${avail_mem}g \\
|
||||
--INPUT ${bam} \\
|
||||
--OUTPUT ${prefix}.bam \\
|
||||
-ID ${ID} \\
|
||||
-LB ${LIBRARY} \\
|
||||
-PL ${PLATFORM} \\
|
||||
-PU ${BARCODE} \\
|
||||
-SM ${SAMPLE} \\
|
||||
-CREATE_INDEX true
|
||||
--RGID ${ID} \\
|
||||
--RGLB ${LIBRARY} \\
|
||||
--RGPL ${PLATFORM} \\
|
||||
--RGPU ${BARCODE} \\
|
||||
--RGSM ${SAMPLE} \\
|
||||
--CREATE_INDEX true
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_CLEANSAM {
|
|||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.9" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.9--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.9--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bam)
|
||||
|
|
@ -31,8 +31,8 @@ process PICARD_CLEANSAM {
|
|||
-Xmx${avail_mem}g \\
|
||||
CleanSam \\
|
||||
${args} \\
|
||||
-I ${bam} \\
|
||||
-O ${prefix}.bam
|
||||
--INPUT ${bam} \\
|
||||
--OUTPUT ${prefix}.bam
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_COLLECTHSMETRICS {
|
|||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bam)
|
||||
|
|
@ -38,10 +38,10 @@ process PICARD_COLLECTHSMETRICS {
|
|||
CollectHsMetrics \\
|
||||
$args \\
|
||||
$reference \\
|
||||
-BAIT_INTERVALS $bait_intervals \\
|
||||
-TARGET_INTERVALS $target_intervals \\
|
||||
-INPUT $bam \\
|
||||
-OUTPUT ${prefix}.CollectHsMetrics.coverage_metrics
|
||||
--BAIT_INTERVALS $bait_intervals \\
|
||||
--TARGET_INTERVALS $target_intervals \\
|
||||
--INPUT $bam \\
|
||||
--OUTPUT ${prefix}.CollectHsMetrics.coverage_metrics
|
||||
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_COLLECTMULTIPLEMETRICS {
|
|||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bam)
|
||||
|
|
@ -33,9 +33,9 @@ process PICARD_COLLECTMULTIPLEMETRICS {
|
|||
-Xmx${avail_mem}g \\
|
||||
CollectMultipleMetrics \\
|
||||
$args \\
|
||||
INPUT=$bam \\
|
||||
OUTPUT=${prefix}.CollectMultipleMetrics \\
|
||||
REFERENCE_SEQUENCE=$fasta
|
||||
--INPUT $bam \\
|
||||
--OUTPUT ${prefix}.CollectMultipleMetrics \\
|
||||
--REFERENCE_SEQUENCE $fasta
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
|||
|
|
@ -2,13 +2,13 @@ process PICARD_COLLECTWGSMETRICS {
|
|||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bam), path(bai)
|
||||
tuple val(meta), path(bam)
|
||||
path fasta
|
||||
|
||||
output:
|
||||
|
|
@ -32,9 +32,10 @@ process PICARD_COLLECTWGSMETRICS {
|
|||
-Xmx${avail_mem}g \\
|
||||
CollectWgsMetrics \\
|
||||
$args \\
|
||||
INPUT=$bam \\
|
||||
OUTPUT=${prefix}.CollectWgsMetrics.coverage_metrics \\
|
||||
REFERENCE_SEQUENCE=$fasta
|
||||
--INPUT $bam \\
|
||||
--OUTPUT ${prefix}.CollectWgsMetrics.coverage_metrics \\
|
||||
--REFERENCE_SEQUENCE $fasta
|
||||
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_CREATESEQUENCEDICTIONARY {
|
|||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.9" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.9--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.9--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(fasta)
|
||||
|
|
@ -31,8 +31,8 @@ process PICARD_CREATESEQUENCEDICTIONARY {
|
|||
-Xmx${avail_mem}g \\
|
||||
CreateSequenceDictionary \\
|
||||
$args \\
|
||||
R=$fasta \\
|
||||
O=${prefix}.dict
|
||||
--REFERENCE $fasta \\
|
||||
--OUTPUT ${prefix}.dict
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_CROSSCHECKFINGERPRINTS {
|
|||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(input1)
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_FILTERSAMREADS {
|
|||
tag "$meta.id"
|
||||
label 'process_low'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bam), path(readlist)
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_FIXMATEINFORMATION {
|
|||
tag "$meta.id"
|
||||
label 'process_low'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.9" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.9--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.9--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bam)
|
||||
|
|
@ -31,8 +31,8 @@ process PICARD_FIXMATEINFORMATION {
|
|||
picard \\
|
||||
FixMateInformation \\
|
||||
-Xmx${avail_mem}g \\
|
||||
-I ${bam} \\
|
||||
-O ${prefix}.bam \\
|
||||
--INPUT ${bam} \\
|
||||
--OUTPUT ${prefix}.bam \\
|
||||
--VALIDATION_STRINGENCY ${STRINGENCY}
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_LIFTOVERVCF {
|
|||
tag "$meta.id"
|
||||
label 'process_low'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(input_vcf)
|
||||
|
|
@ -35,11 +35,11 @@ process PICARD_LIFTOVERVCF {
|
|||
-Xmx${avail_mem}g \\
|
||||
LiftoverVcf \\
|
||||
$args \\
|
||||
I=$input_vcf \\
|
||||
O=${prefix}.lifted.vcf.gz \\
|
||||
CHAIN=$chain \\
|
||||
REJECT=${prefix}.unlifted.vcf.gz \\
|
||||
R=$fasta
|
||||
--INPUT $input_vcf \\
|
||||
--OUTPUT ${prefix}.lifted.vcf.gz \\
|
||||
--CHAIN $chain \\
|
||||
--REJECT ${prefix}.unlifted.vcf.gz \\
|
||||
--REFERENCE_SEQUENCE $fasta
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_MARKDUPLICATES {
|
|||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bam)
|
||||
|
|
@ -33,9 +33,9 @@ process PICARD_MARKDUPLICATES {
|
|||
-Xmx${avail_mem}g \\
|
||||
MarkDuplicates \\
|
||||
$args \\
|
||||
I=$bam \\
|
||||
O=${prefix}.bam \\
|
||||
M=${prefix}.MarkDuplicates.metrics.txt
|
||||
--INPUT $bam \\
|
||||
--OUTPUT ${prefix}.bam \\
|
||||
--METRICS_FILE ${prefix}.MarkDuplicates.metrics.txt
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_MERGESAMFILES {
|
|||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bams)
|
||||
|
|
@ -33,8 +33,8 @@ process PICARD_MERGESAMFILES {
|
|||
-Xmx${avail_mem}g \\
|
||||
MergeSamFiles \\
|
||||
$args \\
|
||||
${'INPUT='+bam_files.join(' INPUT=')} \\
|
||||
OUTPUT=${prefix}.bam
|
||||
${'--INPUT '+bam_files.join(' --INPUT ')} \\
|
||||
--OUTPUT ${prefix}.bam
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
picard: \$( echo \$(picard MergeSamFiles --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:)
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_SORTSAM {
|
|||
tag "$meta.id"
|
||||
label 'process_low'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(bam)
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process PICARD_SORTVCF {
|
|||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::picard=2.26.10" : null)
|
||||
conda (params.enable_conda ? "bioconda::picard=2.27.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.26.10--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.26.10--hdfd78af_0' }"
|
||||
'https://depot.galaxyproject.org/singularity/picard:2.27.1--hdfd78af_0' :
|
||||
'quay.io/biocontainers/picard:2.27.1--hdfd78af_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(vcf)
|
||||
|
|
@ -22,8 +22,8 @@ process PICARD_SORTVCF {
|
|||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
def seq_dict = sequence_dict ? "-SEQUENCE_DICTIONARY $sequence_dict" : ""
|
||||
def reference = reference ? "-REFERENCE_SEQUENCE $reference" : ""
|
||||
def seq_dict = sequence_dict ? "--SEQUENCE_DICTIONARY $sequence_dict" : ""
|
||||
def reference = reference ? "--REFERENCE_SEQUENCE $reference" : ""
|
||||
def avail_mem = 3
|
||||
if (!task.memory) {
|
||||
log.info '[Picard SortVcf] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.'
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process RSEM_CALCULATEEXPRESSION {
|
|||
tag "$meta.id"
|
||||
label 'process_high'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::rsem=1.3.3 bioconda::star=2.7.6a" : null)
|
||||
conda (params.enable_conda ? "bioconda::rsem=1.3.3 bioconda::star=2.7.10a" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:606b713ec440e799d53a2b51a6e79dbfd28ecf3e-0' :
|
||||
'quay.io/biocontainers/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:606b713ec440e799d53a2b51a6e79dbfd28ecf3e-0' }"
|
||||
'https://depot.galaxyproject.org/singularity/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:64aad4a4e144878400649e71f42105311be7ed87-0' :
|
||||
'quay.io/biocontainers/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:64aad4a4e144878400649e71f42105311be7ed87-0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(reads)
|
||||
|
|
|
|||
|
|
@ -2,10 +2,10 @@ process RSEM_PREPAREREFERENCE {
|
|||
tag "$fasta"
|
||||
label 'process_high'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::rsem=1.3.3 bioconda::star=2.7.6a" : null)
|
||||
conda (params.enable_conda ? "bioconda::rsem=1.3.3 bioconda::star=2.7.10a" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:606b713ec440e799d53a2b51a6e79dbfd28ecf3e-0' :
|
||||
'quay.io/biocontainers/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:606b713ec440e799d53a2b51a6e79dbfd28ecf3e-0' }"
|
||||
'https://depot.galaxyproject.org/singularity/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:64aad4a4e144878400649e71f42105311be7ed87-0' :
|
||||
'quay.io/biocontainers/mulled-v2-cf0123ef83b3c38c13e3b0696a3f285d3f20f15b:64aad4a4e144878400649e71f42105311be7ed87-0' }"
|
||||
|
||||
input:
|
||||
path fasta, stageAs: "rsem/*"
|
||||
|
|
|
|||
35
modules/samtools/bamtocram/main.nf
Normal file
35
modules/samtools/bamtocram/main.nf
Normal file
|
|
@ -0,0 +1,35 @@
|
|||
//There is a -L option to only output alignments in interval, might be an option for exons/panel data?
|
||||
process SAMTOOLS_BAMTOCRAM {
|
||||
tag "$meta.id"
|
||||
label 'process_medium'
|
||||
|
||||
conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null)
|
||||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
|
||||
'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' :
|
||||
'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }"
|
||||
|
||||
input:
|
||||
tuple val(meta), path(input), path(index)
|
||||
path fasta
|
||||
path fai
|
||||
|
||||
output:
|
||||
tuple val(meta), path("*.cram"), path("*.crai"), emit: cram_crai
|
||||
path "versions.yml" , emit: versions
|
||||
|
||||
when:
|
||||
task.ext.when == null || task.ext.when
|
||||
|
||||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
"""
|
||||
samtools view --threads ${task.cpus} --reference ${fasta} -C $args $input > ${prefix}.cram
|
||||
samtools index -@${task.cpus} ${prefix}.cram
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//')
|
||||
END_VERSIONS
|
||||
"""
|
||||
}
|
||||
52
modules/samtools/bamtocram/meta.yml
Normal file
52
modules/samtools/bamtocram/meta.yml
Normal file
|
|
@ -0,0 +1,52 @@
|
|||
name: samtools_bamtocram
|
||||
description: filter/convert and then index CRAM file
|
||||
keywords:
|
||||
- view
|
||||
- index
|
||||
- bam
|
||||
- cram
|
||||
tools:
|
||||
- samtools:
|
||||
description: |
|
||||
SAMtools is a set of utilities for interacting with and post-processing
|
||||
short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li.
|
||||
These files are generated as output by short read aligners like BWA.
|
||||
homepage: http://www.htslib.org/
|
||||
documentation: hhttp://www.htslib.org/doc/samtools.html
|
||||
doi: 10.1093/bioinformatics/btp352
|
||||
licence: ["MIT"]
|
||||
input:
|
||||
- meta:
|
||||
type: map
|
||||
description: |
|
||||
Groovy Map containing sample information
|
||||
e.g. [ id:'test', single_end:false ]
|
||||
- input:
|
||||
type: file
|
||||
description: BAM/SAM file
|
||||
pattern: "*.{bam,sam}"
|
||||
- index:
|
||||
type: file
|
||||
description: BAM/SAM index file
|
||||
pattern: "*.{bai,sai}"
|
||||
- fasta:
|
||||
type: file
|
||||
description: Reference file to create the CRAM file
|
||||
pattern: "*.{fasta,fa}"
|
||||
output:
|
||||
- meta:
|
||||
type: map
|
||||
description: |
|
||||
Groovy Map containing sample information
|
||||
e.g. [ id:'test', single_end:false ]
|
||||
- cram_crai:
|
||||
type: file
|
||||
description: filtered/converted CRAM file + index
|
||||
pattern: "*{.cram,.crai}"
|
||||
- version:
|
||||
type: file
|
||||
description: File containing software version
|
||||
pattern: "*.{version.txt}"
|
||||
authors:
|
||||
- "@FriederikeHanssen"
|
||||
- "@maxulysse"
|
||||
|
|
@ -11,7 +11,8 @@ process TABIX_TABIX {
|
|||
tuple val(meta), path(tab)
|
||||
|
||||
output:
|
||||
tuple val(meta), path("*.tbi"), emit: tbi
|
||||
tuple val(meta), path("*.tbi"), optional:true, emit: tbi
|
||||
tuple val(meta), path("*.csi"), optional:true, emit: csi
|
||||
path "versions.yml" , emit: versions
|
||||
|
||||
when:
|
||||
|
|
|
|||
|
|
@ -31,6 +31,10 @@ output:
|
|||
type: file
|
||||
description: tabix index file
|
||||
pattern: "*.{tbi}"
|
||||
- csi:
|
||||
type: file
|
||||
description: coordinate sorted index file
|
||||
pattern: "*.{csi}"
|
||||
- versions:
|
||||
type: file
|
||||
description: File containing software versions
|
||||
|
|
|
|||
|
|
@ -24,7 +24,7 @@ process TIDDIT_SV {
|
|||
script:
|
||||
def args = task.ext.args ?: ''
|
||||
def prefix = task.ext.prefix ?: "${meta.id}"
|
||||
def reference = fasta == "dummy_file.txt" ? "--ref $fasta" : ""
|
||||
def reference = fasta ? "--ref $fasta" : ""
|
||||
"""
|
||||
tiddit \\
|
||||
--sv \\
|
||||
|
|
|
|||
|
|
@ -11,12 +11,13 @@ process TRIMGALORE {
|
|||
tuple val(meta), path(reads)
|
||||
|
||||
output:
|
||||
tuple val(meta), path("*.fq.gz") , emit: reads
|
||||
tuple val(meta), path("*report.txt"), emit: log
|
||||
path "versions.yml" , emit: versions
|
||||
tuple val(meta), path("*{trimmed,val}*.fq.gz"), emit: reads
|
||||
tuple val(meta), path("*report.txt") , emit: log
|
||||
path "versions.yml" , emit: versions
|
||||
|
||||
tuple val(meta), path("*.html"), emit: html optional true
|
||||
tuple val(meta), path("*.zip") , emit: zip optional true
|
||||
tuple val(meta), path("*unpaired*.fq.gz") , emit: unpaired, optional: true
|
||||
tuple val(meta), path("*.html") , emit: html , optional: true
|
||||
tuple val(meta), path("*.zip") , emit: zip , optional: true
|
||||
|
||||
when:
|
||||
task.ext.when == null || task.ext.when
|
||||
|
|
@ -52,6 +53,7 @@ process TRIMGALORE {
|
|||
$c_r1 \\
|
||||
$tpc_r1 \\
|
||||
${prefix}.fastq.gz
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
trimgalore: \$(echo \$(trim_galore --version 2>&1) | sed 's/^.*version //; s/Last.*\$//')
|
||||
|
|
@ -73,6 +75,7 @@ process TRIMGALORE {
|
|||
$tpc_r2 \\
|
||||
${prefix}_1.fastq.gz \\
|
||||
${prefix}_2.fastq.gz
|
||||
|
||||
cat <<-END_VERSIONS > versions.yml
|
||||
"${task.process}":
|
||||
trimgalore: \$(echo \$(trim_galore --version 2>&1) | sed 's/^.*version //; s/Last.*\$//')
|
||||
|
|
|
|||
|
|
@ -37,6 +37,11 @@ output:
|
|||
List of input adapter trimmed FastQ files of size 1 and 2 for
|
||||
single-end and paired-end data, respectively.
|
||||
pattern: "*.{fq.gz}"
|
||||
- unpaired:
|
||||
type: file
|
||||
description: |
|
||||
FastQ files containing unpaired reads from read 1 or read 2
|
||||
pattern: "*unpaired*.fq.gz"
|
||||
- html:
|
||||
type: file
|
||||
description: FastQC report (optional)
|
||||
|
|
|
|||
41
subworkflows/nf-core/bam_qc_picard/main.nf
Normal file
41
subworkflows/nf-core/bam_qc_picard/main.nf
Normal file
|
|
@ -0,0 +1,41 @@
|
|||
//
|
||||
// Run QC steps on BAM/CRAM files using Picard
|
||||
//
|
||||
|
||||
include { PICARD_COLLECTMULTIPLEMETRICS } from '../../../modules/picard/collectmultiplemetrics/main'
|
||||
include { PICARD_COLLECTWGSMETRICS } from '../../../modules/picard/collectwgsmetrics/main'
|
||||
include { PICARD_COLLECTHSMETRICS } from '../../../modules/picard/collecthsmetrics/main'
|
||||
|
||||
workflow BAM_QC_PICARD {
|
||||
take:
|
||||
ch_bam // channel: [ val(meta), [ bam ]]
|
||||
ch_fasta // channel: [ fasta ]
|
||||
ch_fasta_fai // channel: [ fasta_fai ]
|
||||
ch_bait_interval // channel: [ bait_interval ]
|
||||
ch_target_interval // channel: [ target_interval ]
|
||||
|
||||
main:
|
||||
ch_versions = Channel.empty()
|
||||
ch_coverage_metrics = Channel.empty()
|
||||
|
||||
PICARD_COLLECTMULTIPLEMETRICS( ch_bam, ch_fasta )
|
||||
ch_versions = ch_versions.mix(PICARD_COLLECTMULTIPLEMETRICS.out.versions.first())
|
||||
|
||||
if (ch_bait_interval || ch_target_interval) {
|
||||
if (!ch_bait_interval) log.error("Bait interval channel is empty")
|
||||
if (!ch_target_interval) log.error("Target interval channel is empty")
|
||||
PICARD_COLLECTHSMETRICS( ch_bam, ch_fasta, ch_fasta_fai, ch_bait_interval, ch_target_interval )
|
||||
ch_coverage_metrics = ch_coverage_metrics.mix(PICARD_COLLECTHSMETRICS.out.metrics)
|
||||
ch_versions = ch_versions.mix(PICARD_COLLECTHSMETRICS.out.versions.first())
|
||||
} else {
|
||||
PICARD_COLLECTWGSMETRICS( ch_bam, ch_fasta )
|
||||
ch_versions = ch_versions.mix(PICARD_COLLECTWGSMETRICS.out.versions.first())
|
||||
ch_coverage_metrics = ch_coverage_metrics.mix(PICARD_COLLECTWGSMETRICS.out.metrics)
|
||||
}
|
||||
|
||||
emit:
|
||||
coverage_metrics = ch_coverage_metrics // channel: [ val(meta), [ coverage_metrics ] ]
|
||||
multiple_metrics = PICARD_COLLECTMULTIPLEMETRICS.out.metrics // channel: [ val(meta), [ multiple_metrics ] ]
|
||||
|
||||
versions = ch_versions // channel: [ versions.yml ]
|
||||
}
|
||||
60
subworkflows/nf-core/bam_qc_picard/meta.yml
Normal file
60
subworkflows/nf-core/bam_qc_picard/meta.yml
Normal file
|
|
@ -0,0 +1,60 @@
|
|||
name: bam_qc
|
||||
description: Produces comprehensive statistics from BAM file
|
||||
keywords:
|
||||
- statistics
|
||||
- counts
|
||||
- hs_metrics
|
||||
- wgs_metrics
|
||||
- bam
|
||||
- sam
|
||||
- cram
|
||||
modules:
|
||||
- picard/collectmultiplemetrics
|
||||
- picard/collectwgsmetrics
|
||||
- picard/collecthsmetrics
|
||||
input:
|
||||
- meta:
|
||||
type: map
|
||||
description: |
|
||||
Groovy Map containing sample information
|
||||
e.g. [ id:'test', single_end:false ]
|
||||
- bam:
|
||||
type: file
|
||||
description: BAM/CRAM/SAM file
|
||||
pattern: "*.{bam,cram,sam}"
|
||||
- fasta:
|
||||
type: optional file
|
||||
description: Reference fasta file
|
||||
pattern: "*.{fasta,fa}"
|
||||
- fasta_fai:
|
||||
type: optional file
|
||||
description: Reference fasta file index
|
||||
pattern: "*.{fasta,fa}.fai"
|
||||
- bait_intervals:
|
||||
type: optional file
|
||||
description: An interval list file that contains the locations of the baits used.
|
||||
pattern: "baits.interval_list"
|
||||
- target_intervals:
|
||||
type: optional file
|
||||
description: An interval list file that contains the locations of the targets.
|
||||
pattern: "targets.interval_list"
|
||||
output:
|
||||
- meta:
|
||||
type: map
|
||||
description: |
|
||||
Groovy Map containing sample information
|
||||
e.g. [ id:'test', single_end:false ]
|
||||
- coverage_metrics:
|
||||
type: file
|
||||
description: Alignment metrics files generated by picard CollectHsMetrics or CollectWgsMetrics
|
||||
pattern: "*_metrics.txt"
|
||||
- multiple_metrics:
|
||||
type: file
|
||||
description: Alignment metrics files generated by picard CollectMultipleMetrics
|
||||
pattern: "*_{metrics}"
|
||||
- versions:
|
||||
type: file
|
||||
description: File containing software versions
|
||||
pattern: "versions.yml"
|
||||
authors:
|
||||
- "@matthdsm"
|
||||
|
|
@ -607,6 +607,10 @@ elprep/filter:
|
|||
- modules/elprep/filter/**
|
||||
- tests/modules/elprep/filter/**
|
||||
|
||||
elprep/merge:
|
||||
- modules/elprep/merge/**
|
||||
- tests/modules/elprep/merge/**
|
||||
|
||||
elprep/split:
|
||||
- modules/elprep/split/**
|
||||
- tests/modules/elprep/split/**
|
||||
|
|
@ -1054,6 +1058,10 @@ krona/ktimporttaxonomy:
|
|||
- modules/krona/ktimporttaxonomy/**
|
||||
- tests/modules/krona/ktimporttaxonomy/**
|
||||
|
||||
krona/ktimporttext:
|
||||
- modules/krona/ktimporttext/**
|
||||
- tests/modules/krona/ktimporttext/**
|
||||
|
||||
last/dotplot:
|
||||
- modules/last/dotplot/**
|
||||
- tests/modules/last/dotplot/**
|
||||
|
|
@ -1599,6 +1607,10 @@ samtools/bam2fq:
|
|||
- modules/samtools/bam2fq/**
|
||||
- tests/modules/samtools/bam2fq/**
|
||||
|
||||
samtools/bamtocram:
|
||||
- modules/samtools/bamtocram/**
|
||||
- tests/modules/samtools/bamtocram/**
|
||||
|
||||
samtools/collatefastq:
|
||||
- modules/samtools/collatefastq/**
|
||||
- tests/modules/samtools/collatefastq/**
|
||||
|
|
|
|||
|
|
@ -14,6 +14,7 @@ params {
|
|||
genome_paf = "${test_data_dir}/genomics/sarscov2/genome/genome.paf"
|
||||
genome_sizes = "${test_data_dir}/genomics/sarscov2/genome/genome.sizes"
|
||||
transcriptome_fasta = "${test_data_dir}/genomics/sarscov2/genome/transcriptome.fasta"
|
||||
proteome_fasta = "${test_data_dir}/genomics/sarscov2/genome/proteome.fasta"
|
||||
transcriptome_paf = "${test_data_dir}/genomics/sarscov2/genome/transcriptome.paf"
|
||||
|
||||
test_bed = "${test_data_dir}/genomics/sarscov2/genome/bed/test.bed"
|
||||
|
|
@ -109,6 +110,9 @@ params {
|
|||
|
||||
test_sequencing_summary = "${test_data_dir}/genomics/sarscov2/nanopore/sequencing_summary/test.sequencing_summary.txt"
|
||||
}
|
||||
'metagenome' {
|
||||
kraken_report = "${test_data_dir}/genomics/sarscov2/metagenome/test_1.kraken2.report.txt"
|
||||
}
|
||||
}
|
||||
'homo_sapiens' {
|
||||
'genome' {
|
||||
|
|
@ -245,8 +249,8 @@ params {
|
|||
test2_2_fastq_gz = "${test_data_dir}/genomics/homo_sapiens/illumina/fastq/test2_2.fastq.gz"
|
||||
test2_umi_1_fastq_gz = "${test_data_dir}/genomics/homo_sapiens/illumina/fastq/test2.umi_1.fastq.gz"
|
||||
test2_umi_2_fastq_gz = "${test_data_dir}/genomics/homo_sapiens/illumina/fastq/test2.umi_2.fastq.gz"
|
||||
test_rnaseq_1_fastq_gz = "${test_data_dir}/genomics/homo_sapiens/illumina/fastq/test.rnaseq_1.fastq.gz"
|
||||
test_rnaseq_2_fastq_gz = "${test_data_dir}/genomics/homo_sapiens/illumina/fastq/test.rnaseq_2.fastq.gz"
|
||||
test_rnaseq_1_fastq_gz = "${test_data_dir}/genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz"
|
||||
test_rnaseq_2_fastq_gz = "${test_data_dir}/genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz"
|
||||
|
||||
test_baserecalibrator_table = "${test_data_dir}/genomics/homo_sapiens/illumina/gatk/test.baserecalibrator.table"
|
||||
test2_baserecalibrator_table = "${test_data_dir}/genomics/homo_sapiens/illumina/gatk/test2.baserecalibrator.table"
|
||||
|
|
|
|||
|
|
@ -1,14 +1,17 @@
|
|||
- name: antismash antismashlitedownloaddatabases test_antismash_antismashlitedownloaddatabases
|
||||
command: nextflow run tests/modules/antismash/antismashlitedownloaddatabases -entry test_antismash_antismashlitedownloaddatabases -c tests/config/nextflow.config
|
||||
tags:
|
||||
- antismash/antismashlitedownloaddatabases
|
||||
- antismash
|
||||
- antismash/antismashlitedownloaddatabases
|
||||
files:
|
||||
- path: output/antismash/versions.yml
|
||||
md5sum: e2656c8d2bcc7469eba40eb1ee5c91b3
|
||||
md5sum: 24859c67023abab99de295d3675a24b6
|
||||
- path: output/antismash/antismash_db
|
||||
- path: output/antismash/antismash_db/clusterblast
|
||||
- path: output/antismash/antismash_db/clustercompare
|
||||
- path: output/antismash/antismash_db/pfam
|
||||
- path: output/antismash/antismash_db/resfam
|
||||
- path: output/antismash/antismash_db/tigrfam
|
||||
- path: output/antismash/css
|
||||
- path: output/antismash/detection
|
||||
- path: output/antismash/modules
|
||||
|
|
|
|||
|
|
@ -2,13 +2,29 @@
|
|||
|
||||
nextflow.enable.dsl = 2
|
||||
|
||||
include { BAMTOOLS_SPLIT } from '../../../../modules/bamtools/split/main.nf'
|
||||
include { BAMTOOLS_SPLIT as BAMTOOLS_SPLIT_SINGLE } from '../../../../modules/bamtools/split/main.nf'
|
||||
include { BAMTOOLS_SPLIT as BAMTOOLS_SPLIT_MULTIPLE } from '../../../../modules/bamtools/split/main.nf'
|
||||
|
||||
workflow test_bamtools_split {
|
||||
workflow test_bamtools_split_single_input {
|
||||
|
||||
input = [
|
||||
[ id:'test', single_end:false ], // meta map
|
||||
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) ]
|
||||
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
|
||||
]
|
||||
|
||||
BAMTOOLS_SPLIT ( input )
|
||||
BAMTOOLS_SPLIT_SINGLE ( input )
|
||||
}
|
||||
|
||||
workflow test_bamtools_split_multiple {
|
||||
|
||||
input = [
|
||||
[ id:'test', single_end:false ], // meta map
|
||||
[
|
||||
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
|
||||
file(params.test_data['homo_sapiens']['illumina']['test2_paired_end_sorted_bam'], checkIfExists: true)
|
||||
]
|
||||
]
|
||||
|
||||
BAMTOOLS_SPLIT_MULTIPLE ( input )
|
||||
}
|
||||
|
||||
|
|
|
|||
|
|
@ -1,10 +1,23 @@
|
|||
- name: bamtools split test_bamtools_split
|
||||
command: nextflow run ./tests/modules/bamtools/split -entry test_bamtools_split -c ./tests/config/nextflow.config -c ./tests/modules/bamtools/split/nextflow.config
|
||||
- name: bamtools split test_bamtools_split_single_input
|
||||
command: nextflow run ./tests/modules/bamtools/split -entry test_bamtools_split_single_input -c ./tests/config/nextflow.config -c ./tests/modules/bamtools/split/nextflow.config
|
||||
tags:
|
||||
- bamtools/split
|
||||
- bamtools
|
||||
- bamtools/split
|
||||
files:
|
||||
- path: output/bamtools/test.paired_end.sorted.REF_chr22.bam
|
||||
- path: output/bamtools/test.REF_chr22.bam
|
||||
md5sum: b7dc50e0edf9c6bfc2e3b0e6d074dc07
|
||||
- path: output/bamtools/test.paired_end.sorted.REF_unmapped.bam
|
||||
- path: output/bamtools/test.REF_unmapped.bam
|
||||
md5sum: e0754bf72c51543b2d745d96537035fb
|
||||
- path: output/bamtools/versions.yml
|
||||
|
||||
- name: bamtools split test_bamtools_split_multiple
|
||||
command: nextflow run ./tests/modules/bamtools/split -entry test_bamtools_split_multiple -c ./tests/config/nextflow.config -c ./tests/modules/bamtools/split/nextflow.config
|
||||
tags:
|
||||
- bamtools
|
||||
- bamtools/split
|
||||
files:
|
||||
- path: output/bamtools/test.REF_chr22.bam
|
||||
md5sum: 585675bea34c48ebe9db06a561d4b4fa
|
||||
- path: output/bamtools/test.REF_unmapped.bam
|
||||
md5sum: 16ad644c87b9471f3026bc87c98b4963
|
||||
- path: output/bamtools/versions.yml
|
||||
|
|
|
|||
|
|
@ -7,9 +7,22 @@ include { DIAMOND_BLASTP } from '../../../../modules/diamond/blastp/main.nf'
|
|||
|
||||
workflow test_diamond_blastp {
|
||||
|
||||
db = [ file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ]
|
||||
fasta = [ file(params.test_data['sarscov2']['genome']['transcriptome_fasta'], checkIfExists: true) ]
|
||||
db = [ file(params.test_data['sarscov2']['genome']['proteome_fasta'], checkIfExists: true) ]
|
||||
fasta = [ file(params.test_data['sarscov2']['genome']['proteome_fasta'], checkIfExists: true) ]
|
||||
out_ext = 'txt'
|
||||
blast_columns = 'qseqid qlen'
|
||||
|
||||
DIAMOND_MAKEDB ( db )
|
||||
DIAMOND_BLASTP ( [ [id:'test'], fasta ], DIAMOND_MAKEDB.out.db )
|
||||
DIAMOND_BLASTP ( [ [id:'test'], fasta ], DIAMOND_MAKEDB.out.db, out_ext, blast_columns )
|
||||
}
|
||||
|
||||
workflow test_diamond_blastp_daa {
|
||||
|
||||
db = [ file(params.test_data['sarscov2']['genome']['proteome_fasta'], checkIfExists: true) ]
|
||||
fasta = [ file(params.test_data['sarscov2']['genome']['proteome_fasta'], checkIfExists: true) ]
|
||||
out_ext = 'daa'
|
||||
blast_columns = []
|
||||
|
||||
DIAMOND_MAKEDB ( db )
|
||||
DIAMOND_BLASTP ( [ [id:'test'], fasta ], DIAMOND_MAKEDB.out.db, out_ext, blast_columns )
|
||||
}
|
||||
|
|
|
|||
|
|
@ -1,8 +1,17 @@
|
|||
- name: diamond blastp
|
||||
command: nextflow run ./tests/modules/diamond/blastp -entry test_diamond_blastp -c ./tests/config/nextflow.config -c ./tests/modules/diamond/blastp/nextflow.config
|
||||
- name: diamond blastp test_diamond_blastp
|
||||
command: nextflow run tests/modules/diamond/blastp -entry test_diamond_blastp -c tests/config/nextflow.config
|
||||
tags:
|
||||
- diamond
|
||||
- diamond/blastp
|
||||
- diamond
|
||||
files:
|
||||
- path: ./output/diamond/test.diamond_blastp.txt
|
||||
md5sum: 3ca7f6290c1d8741c573370e6f8b4db0
|
||||
- path: output/diamond/test.diamond_blastp.txt
|
||||
- path: output/diamond/versions.yml
|
||||
|
||||
- name: diamond blastp test_diamond_blastp_daa
|
||||
command: nextflow run tests/modules/diamond/blastp -entry test_diamond_blastp_daa -c tests/config/nextflow.config
|
||||
tags:
|
||||
- diamond/blastp
|
||||
- diamond
|
||||
files:
|
||||
- path: output/diamond/test.diamond_blastp.daa
|
||||
- path: output/diamond/versions.yml
|
||||
|
|
|
|||
|
|
@ -7,9 +7,22 @@ include { DIAMOND_BLASTX } from '../../../../modules/diamond/blastx/main.nf'
|
|||
|
||||
workflow test_diamond_blastx {
|
||||
|
||||
db = [ file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ]
|
||||
db = [ file(params.test_data['sarscov2']['genome']['proteome_fasta'], checkIfExists: true) ]
|
||||
fasta = [ file(params.test_data['sarscov2']['genome']['transcriptome_fasta'], checkIfExists: true) ]
|
||||
out_ext = 'tfdfdt' // Nonsense file extension to check default case.
|
||||
blast_columns = 'qseqid qlen'
|
||||
|
||||
DIAMOND_MAKEDB ( db )
|
||||
DIAMOND_BLASTX ( [ [id:'test'], fasta ], DIAMOND_MAKEDB.out.db )
|
||||
DIAMOND_BLASTX ( [ [id:'test'], fasta ], DIAMOND_MAKEDB.out.db, out_ext, blast_columns )
|
||||
}
|
||||
|
||||
workflow test_diamond_blastx_daa {
|
||||
|
||||
db = [ file(params.test_data['sarscov2']['genome']['proteome_fasta'], checkIfExists: true) ]
|
||||
fasta = [ file(params.test_data['sarscov2']['genome']['transcriptome_fasta'], checkIfExists: true) ]
|
||||
out_ext = 'daa'
|
||||
blast_columns = []
|
||||
|
||||
DIAMOND_MAKEDB ( db )
|
||||
DIAMOND_BLASTX ( [ [id:'test'], fasta ], DIAMOND_MAKEDB.out.db, out_ext, blast_columns )
|
||||
}
|
||||
|
|
|
|||
|
|
@ -1,8 +1,18 @@
|
|||
- name: diamond blastx
|
||||
command: nextflow run ./tests/modules/diamond/blastx -entry test_diamond_blastx -c ./tests/config/nextflow.config -c ./tests/modules/diamond/blastx/nextflow.config
|
||||
- name: diamond blastx test_diamond_blastx
|
||||
command: nextflow run tests/modules/diamond/blastx -entry test_diamond_blastx -c tests/config/nextflow.config
|
||||
tags:
|
||||
- diamond
|
||||
- diamond/blastx
|
||||
files:
|
||||
- path: ./output/diamond/test.diamond_blastx.txt
|
||||
md5sum: d41d8cd98f00b204e9800998ecf8427e
|
||||
- path: output/diamond/test.diamond_blastx.txt
|
||||
- path: output/diamond/versions.yml
|
||||
|
||||
- name: diamond blastx test_diamond_blastx_daa
|
||||
command: nextflow run tests/modules/diamond/blastx -entry test_diamond_blastx_daa -c tests/config/nextflow.config
|
||||
tags:
|
||||
- diamond
|
||||
- diamond/blastx
|
||||
files:
|
||||
- path: output/diamond/test.diamond_blastx.daa
|
||||
md5sum: 0df4a833408416f32981415873facc11
|
||||
- path: output/diamond/versions.yml
|
||||
|
|
|
|||
|
|
@ -6,7 +6,7 @@ include { DIAMOND_MAKEDB } from '../../../../modules/diamond/makedb/main.nf'
|
|||
|
||||
workflow test_diamond_makedb {
|
||||
|
||||
input = [ file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ]
|
||||
input = [ file(params.test_data['sarscov2']['genome']['proteome_fasta'], checkIfExists: true) ]
|
||||
|
||||
DIAMOND_MAKEDB ( input )
|
||||
}
|
||||
|
|
|
|||
|
|
@ -1,8 +1,9 @@
|
|||
- name: diamond makedb test_diamond_makedb
|
||||
command: nextflow run ./tests/modules/diamond/makedb -entry test_diamond_makedb -c ./tests/config/nextflow.config -c ./tests/modules/diamond/makedb/nextflow.config
|
||||
command: nextflow run tests/modules/diamond/makedb -entry test_diamond_makedb -c tests/config/nextflow.config
|
||||
tags:
|
||||
- diamond
|
||||
- diamond/makedb
|
||||
- diamond
|
||||
files:
|
||||
- path: output/diamond/genome.fasta.dmnd
|
||||
md5sum: 2447fb376394c20d43ea3aad2aa5d15d
|
||||
- path: output/diamond/proteome.fasta.dmnd
|
||||
md5sum: fc28c50b202dd7a7c5451cddff2ba1f4
|
||||
- path: output/diamond/versions.yml
|
||||
|
|
|
|||
17
tests/modules/elprep/merge/main.nf
Normal file
17
tests/modules/elprep/merge/main.nf
Normal file
|
|
@ -0,0 +1,17 @@
|
|||
#!/usr/bin/env nextflow
|
||||
|
||||
nextflow.enable.dsl = 2
|
||||
|
||||
include { ELPREP_SPLIT } from '../../../../modules/elprep/split/main.nf'
|
||||
include { ELPREP_MERGE } from '../../../../modules/elprep/merge/main.nf'
|
||||
|
||||
workflow test_elprep_merge {
|
||||
|
||||
input = [
|
||||
[ id:'test', single_end:false ], // meta map
|
||||
file(params.test_data['homo_sapiens']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
|
||||
]
|
||||
|
||||
ELPREP_SPLIT ( input )
|
||||
ELPREP_MERGE ( ELPREP_SPLIT.out.bam )
|
||||
}
|
||||
5
tests/modules/elprep/merge/nextflow.config
Normal file
5
tests/modules/elprep/merge/nextflow.config
Normal file
|
|
@ -0,0 +1,5 @@
|
|||
process {
|
||||
withName : ELPREP_MERGE {
|
||||
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
|
||||
}
|
||||
}
|
||||
8
tests/modules/elprep/merge/test.yml
Normal file
8
tests/modules/elprep/merge/test.yml
Normal file
|
|
@ -0,0 +1,8 @@
|
|||
- name: elprep merge test_elprep_merge
|
||||
command: nextflow run tests/modules/elprep/merge -entry test_elprep_merge -c tests/config/nextflow.config
|
||||
tags:
|
||||
- elprep
|
||||
- elprep/merge
|
||||
files:
|
||||
- path: output/elprep/output/test.bam
|
||||
- path: output/elprep/versions.yml
|
||||
|
|
@ -6,7 +6,23 @@ include { GATK4_SPLITNCIGARREADS } from '../../../../modules/gatk4/splitncigarre
|
|||
|
||||
workflow test_gatk4_splitncigarreads {
|
||||
input = [ [ id:'test' ], // meta map
|
||||
[ file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true) ]
|
||||
file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true),
|
||||
[],
|
||||
[]
|
||||
]
|
||||
|
||||
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
||||
fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
|
||||
dict = file(params.test_data['sarscov2']['genome']['genome_dict'], checkIfExists: true)
|
||||
|
||||
GATK4_SPLITNCIGARREADS ( input, fasta, fai, dict )
|
||||
}
|
||||
|
||||
workflow test_gatk4_splitncigarreads_intervals {
|
||||
input = [ [ id:'test' ], // meta map
|
||||
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
|
||||
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true),
|
||||
file(params.test_data['sarscov2']['genome']['test_bed'], checkIfExists: true)
|
||||
]
|
||||
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
||||
fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
|
||||
|
|
|
|||
|
|
@ -5,5 +5,14 @@
|
|||
- gatk4/splitncigarreads
|
||||
files:
|
||||
- path: output/gatk4/test.bam
|
||||
md5sum: ceed15c0bd64ff5c38d3816905933b0b
|
||||
md5sum: 436d8e31285c6b588bdd1c7f1d07f6f2
|
||||
- path: output/gatk4/versions.yml
|
||||
- name: gatk4 splitncigarreads test_gatk4_splitncigarreads_intervals
|
||||
command: nextflow run tests/modules/gatk4/splitncigarreads -entry test_gatk4_splitncigarreads_intervals -c tests/config/nextflow.config
|
||||
tags:
|
||||
- gatk4
|
||||
- gatk4/splitncigarreads
|
||||
files:
|
||||
- path: output/gatk4/test.bam
|
||||
md5sum: cd56e3225950f519fd47164cca60a0bb
|
||||
- path: output/gatk4/versions.yml
|
||||
|
|
|
|||
31
tests/modules/krona/ktimporttext/main.nf
Normal file
31
tests/modules/krona/ktimporttext/main.nf
Normal file
|
|
@ -0,0 +1,31 @@
|
|||
#!/usr/bin/env nextflow
|
||||
|
||||
nextflow.enable.dsl = 2
|
||||
|
||||
include { KRONA_KTIMPORTTEXT } from '../../../../modules/krona/ktimporttext/main.nf'
|
||||
|
||||
workflow test_krona_ktimporttext_multi {
|
||||
|
||||
input = [
|
||||
[ id:'test', single_end:false ], // meta map
|
||||
[
|
||||
file('https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/delete_me/krona/ktimporttext.txt', checkIfExists: true), // krona default test file
|
||||
file(params.test_data['sarscov2']['metagenome']['kraken_report'], checkIfExists: true), //Kraken2 report file
|
||||
file('https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/delete_me/krona/kaiju_out4krona.txt', checkIfExists: true) // Kaiju output 4 krona
|
||||
]
|
||||
]
|
||||
|
||||
KRONA_KTIMPORTTEXT ( input )
|
||||
}
|
||||
|
||||
workflow test_krona_ktimporttext_single {
|
||||
|
||||
input = [
|
||||
[ id:'test', single_end:false ], // meta map
|
||||
[
|
||||
file('http://krona.sourceforge.net/examples/text.txt', checkIfExists: true) // krona default test file
|
||||
]
|
||||
]
|
||||
|
||||
KRONA_KTIMPORTTEXT ( input )
|
||||
}
|
||||
5
tests/modules/krona/ktimporttext/nextflow.config
Normal file
5
tests/modules/krona/ktimporttext/nextflow.config
Normal file
|
|
@ -0,0 +1,5 @@
|
|||
process {
|
||||
|
||||
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
|
||||
|
||||
}
|
||||
19
tests/modules/krona/ktimporttext/test.yml
Normal file
19
tests/modules/krona/ktimporttext/test.yml
Normal file
|
|
@ -0,0 +1,19 @@
|
|||
- name: krona ktimporttext test_krona_ktimporttext_multi
|
||||
command: nextflow run tests/modules/krona/ktimporttext -entry test_krona_ktimporttext_multi -c tests/config/nextflow.config
|
||||
tags:
|
||||
- krona
|
||||
- krona/ktimporttext
|
||||
files:
|
||||
- path: output/krona/test.html
|
||||
contains:
|
||||
- "DOCTYPE html PUBLIC"
|
||||
|
||||
- name: krona ktimporttext test_krona_ktimporttext_single
|
||||
command: nextflow run tests/modules/krona/ktimporttext -entry test_krona_ktimporttext_single -c tests/config/nextflow.config
|
||||
tags:
|
||||
- krona
|
||||
- krona/ktimporttext
|
||||
files:
|
||||
- path: output/krona/test.html
|
||||
contains:
|
||||
- "DOCTYPE html PUBLIC"
|
||||
|
|
@ -9,8 +9,11 @@ workflow test_minimap2_align_single_end {
|
|||
[ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)]
|
||||
]
|
||||
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
||||
bam_format = true
|
||||
cigar_paf_format = false
|
||||
cigar_bam = false
|
||||
|
||||
MINIMAP2_ALIGN ( input, fasta )
|
||||
MINIMAP2_ALIGN ( input, fasta, bam_format, cigar_paf_format, cigar_bam)
|
||||
}
|
||||
|
||||
workflow test_minimap2_align_paired_end {
|
||||
|
|
@ -19,6 +22,9 @@ workflow test_minimap2_align_paired_end {
|
|||
file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ]
|
||||
]
|
||||
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
||||
bam_format = true
|
||||
cigar_paf_format = false
|
||||
cigar_bam = false
|
||||
|
||||
MINIMAP2_ALIGN ( input, fasta )
|
||||
MINIMAP2_ALIGN ( input, fasta, bam_format, cigar_paf_format, cigar_bam )
|
||||
}
|
||||
|
|
|
|||
|
|
@ -1,17 +1,17 @@
|
|||
- name: minimap2 align single-end
|
||||
command: nextflow run ./tests/modules/minimap2/align -entry test_minimap2_align_single_end -c ./tests/config/nextflow.config -c ./tests/modules/minimap2/align/nextflow.config
|
||||
- name: minimap2 align test_minimap2_align_single_end
|
||||
command: nextflow run tests/modules/minimap2/align -entry test_minimap2_align_single_end -c tests/config/nextflow.config
|
||||
tags:
|
||||
- minimap2
|
||||
- minimap2/align
|
||||
files:
|
||||
- path: ./output/minimap2/test.paf
|
||||
md5sum: 70e8cf299ee3ecd33e629d10c1f588ce
|
||||
- path: output/minimap2/test.bam
|
||||
- path: output/minimap2/versions.yml
|
||||
|
||||
- name: minimap2 align paired-end
|
||||
command: nextflow run ./tests/modules/minimap2/align -entry test_minimap2_align_paired_end -c ./tests/config/nextflow.config -c ./tests/modules/minimap2/align/nextflow.config
|
||||
- name: minimap2 align test_minimap2_align_paired_end
|
||||
command: nextflow run tests/modules/minimap2/align -entry test_minimap2_align_paired_end -c tests/config/nextflow.config
|
||||
tags:
|
||||
- minimap2
|
||||
- minimap2/align
|
||||
files:
|
||||
- path: ./output/minimap2/test.paf
|
||||
md5sum: 5e7b55a26bf0ea3a2843423d3e0b9a28
|
||||
- path: output/minimap2/test.bam
|
||||
- path: output/minimap2/versions.yml
|
||||
|
|
|
|||
|
|
@ -7,4 +7,3 @@
|
|||
- path: output/picard/test.bam
|
||||
md5sum: 7b82f3461c2d80fc6a10385e78c9427f
|
||||
- path: output/picard/versions.yml
|
||||
md5sum: 8a2d176295e1343146ea433c79bb517f
|
||||
|
|
|
|||
|
|
@ -7,4 +7,3 @@
|
|||
- path: output/picard/test.bam
|
||||
md5sum: a48f8e77a1480445efc57570c3a38a68
|
||||
- path: output/picard/versions.yml
|
||||
md5sum: e6457d7c6de51bf6f4b577eda65e57ac
|
||||
|
|
|
|||
|
|
@ -6,8 +6,7 @@ include { PICARD_COLLECTWGSMETRICS } from '../../../../modules/picard/collectwgs
|
|||
|
||||
workflow test_picard_collectwgsmetrics {
|
||||
input = [ [ id:'test', single_end:false ], // meta map
|
||||
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
|
||||
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)
|
||||
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
|
||||
]
|
||||
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
||||
|
||||
|
|
|
|||
|
|
@ -7,4 +7,3 @@
|
|||
- path: output/picard/test.dict
|
||||
contains: ["SN:MT192765.1"]
|
||||
- path: output/picard/versions.yml
|
||||
md5sum: b3d8c7ea65b8a6d3237b153d13fe2014
|
||||
|
|
|
|||
|
|
@ -7,4 +7,3 @@
|
|||
- path: output/picard/test.bam
|
||||
md5sum: 746102e8c242c0ef42e045c49d320030
|
||||
- path: output/picard/versions.yml
|
||||
md5sum: 4329ba7cdca8f4f6018dfd5c019ba2eb
|
||||
|
|
|
|||
|
|
@ -1,5 +1,5 @@
|
|||
process {
|
||||
ext.args = "WARN_ON_MISSING_CONTIG=true"
|
||||
ext.args = "--WARN_ON_MISSING_CONTIG true"
|
||||
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
|
||||
|
||||
}
|
||||
|
|
|
|||
|
|
@ -3,7 +3,7 @@ process {
|
|||
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
|
||||
|
||||
withName: PICARD_MARKDUPLICATES_UNSORTED {
|
||||
ext.args = 'ASSUME_SORT_ORDER=queryname'
|
||||
ext.args = '--ASSUME_SORT_ORDER queryname'
|
||||
}
|
||||
|
||||
}
|
||||
|
|
|
|||
|
|
@ -42,7 +42,7 @@
|
|||
- path: output/rsem/rsem/genome.transcripts.fa
|
||||
md5sum: 050c521a2719c2ae48267c1e65218f29
|
||||
- path: output/rsem/rsem/genomeParameters.txt
|
||||
md5sum: 2fe3a030e1706c3e8cd4df3818e6dd2f
|
||||
md5sum: df5a456e3242520cc36e0083a6a7d9dd
|
||||
- path: output/rsem/rsem/sjdbInfo.txt
|
||||
md5sum: 5690ea9d9f09f7ff85b7fd47bd234903
|
||||
- path: output/rsem/rsem/sjdbList.fromGTF.out.tab
|
||||
|
|
@ -63,4 +63,4 @@
|
|||
- path: output/rsem/test.stat/test.theta
|
||||
md5sum: de2e4490c98cc5383a86ae8225fd0a28
|
||||
- path: output/rsem/test.transcript.bam
|
||||
md5sum: 7846491086c478858419667d60f18edd
|
||||
md5sum: ed681d39f5700ffc74d6321525330d93
|
||||
|
|
|
|||
17
tests/modules/samtools/bamtocram/main.nf
Normal file
17
tests/modules/samtools/bamtocram/main.nf
Normal file
|
|
@ -0,0 +1,17 @@
|
|||
#!/usr/bin/env nextflow
|
||||
|
||||
nextflow.enable.dsl = 2
|
||||
|
||||
include { SAMTOOLS_BAMTOCRAM } from '../../../../modules/samtools/bamtocram/main.nf'
|
||||
|
||||
workflow test_samtools_bamtocram {
|
||||
|
||||
input = [ [ id:'test', single_end:false ], // meta map
|
||||
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true),
|
||||
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true)]
|
||||
|
||||
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
||||
fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
|
||||
|
||||
SAMTOOLS_BAMTOCRAM ( input, fasta, fai )
|
||||
}
|
||||
5
tests/modules/samtools/bamtocram/nextflow.config
Normal file
5
tests/modules/samtools/bamtocram/nextflow.config
Normal file
|
|
@ -0,0 +1,5 @@
|
|||
process {
|
||||
|
||||
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
|
||||
|
||||
}
|
||||
9
tests/modules/samtools/bamtocram/test.yml
Normal file
9
tests/modules/samtools/bamtocram/test.yml
Normal file
|
|
@ -0,0 +1,9 @@
|
|||
- name: samtools bamtocram test_samtools_bamtocram
|
||||
command: nextflow run ./tests/modules/samtools/bamtocram -entry test_samtools_bamtocram -c ./tests/config/nextflow.config -c ./tests/modules/samtools/bamtocram/nextflow.config
|
||||
tags:
|
||||
- samtools/bamtocram
|
||||
- samtools
|
||||
files:
|
||||
- path: output/samtools/test.cram
|
||||
- path: output/samtools/test.cram.crai
|
||||
- path: output/samtools/versions.yml
|
||||
|
|
@ -2,9 +2,10 @@
|
|||
|
||||
nextflow.enable.dsl = 2
|
||||
|
||||
include { TABIX_TABIX as TABIX_BED } from '../../../../modules/tabix/tabix/main.nf'
|
||||
include { TABIX_TABIX as TABIX_GFF } from '../../../../modules/tabix/tabix/main.nf'
|
||||
include { TABIX_TABIX as TABIX_VCF } from '../../../../modules/tabix/tabix/main.nf'
|
||||
include { TABIX_TABIX as TABIX_BED } from '../../../../modules/tabix/tabix/main.nf'
|
||||
include { TABIX_TABIX as TABIX_GFF } from '../../../../modules/tabix/tabix/main.nf'
|
||||
include { TABIX_TABIX as TABIX_VCF_TBI } from '../../../../modules/tabix/tabix/main.nf'
|
||||
include { TABIX_TABIX as TABIX_VCF_CSI } from '../../../../modules/tabix/tabix/main.nf'
|
||||
|
||||
workflow test_tabix_tabix_bed {
|
||||
input = [ [ id:'B.bed' ], // meta map
|
||||
|
|
@ -22,10 +23,18 @@ workflow test_tabix_tabix_gff {
|
|||
TABIX_GFF ( input )
|
||||
}
|
||||
|
||||
workflow test_tabix_tabix_vcf {
|
||||
workflow test_tabix_tabix_vcf_tbi {
|
||||
input = [ [ id:'test.vcf' ], // meta map
|
||||
[ file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true) ]
|
||||
]
|
||||
|
||||
TABIX_VCF ( input )
|
||||
TABIX_VCF_TBI ( input )
|
||||
}
|
||||
|
||||
workflow test_tabix_tabix_vcf_csi {
|
||||
input = [ [ id:'test.vcf' ], // meta map
|
||||
[ file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true) ]
|
||||
]
|
||||
|
||||
TABIX_VCF_CSI ( input )
|
||||
}
|
||||
|
|
|
|||
|
|
@ -10,8 +10,12 @@ process {
|
|||
ext.args = '-p gff'
|
||||
}
|
||||
|
||||
withName: TABIX_VCF {
|
||||
withName: TABIX_VCF_TBI {
|
||||
ext.args = '-p vcf'
|
||||
}
|
||||
|
||||
withName: TABIX_VCF_CSI {
|
||||
ext.args = '-p vcf --csi'
|
||||
}
|
||||
|
||||
}
|
||||
|
|
|
|||
|
|
@ -15,10 +15,18 @@
|
|||
- path: ./output/tabix/genome.gff3.gz.tbi
|
||||
md5sum: f79a67d95a98076e04fbe0455d825926
|
||||
- name: tabix tabix vcf
|
||||
command: nextflow run ./tests/modules/tabix/tabix -entry test_tabix_tabix_vcf -c ./tests/config/nextflow.config -c ./tests/modules/tabix/tabix/nextflow.config
|
||||
command: nextflow run ./tests/modules/tabix/tabix -entry test_tabix_tabix_vcf_tbi -c ./tests/config/nextflow.config -c ./tests/modules/tabix/tabix/nextflow.config
|
||||
tags:
|
||||
- tabix
|
||||
- tabix/tabix
|
||||
files:
|
||||
- path: output/tabix/test.vcf.gz.tbi
|
||||
md5sum: 36e11bf96ed0af4a92caa91a68612d64
|
||||
- name: tabix tabix vcf csi
|
||||
command: nextflow run ./tests/modules/tabix/tabix -entry test_tabix_tabix_vcf_csi -c ./tests/config/nextflow.config -c ./tests/modules/tabix/tabix/nextflow.config
|
||||
tags:
|
||||
- tabix
|
||||
- tabix/tabix
|
||||
files:
|
||||
- path: output/tabix/test.vcf.gz.csi
|
||||
md5sum: 5f930522d2b9dcdba2807b7da4dfa3fd
|
||||
|
|
|
|||
|
|
@ -9,6 +9,7 @@
|
|||
- path: output/tiddit/test.signals.tab
|
||||
md5sum: dab4b2fec4ddf8eb1c23005b0770150e
|
||||
- path: output/tiddit/test.vcf
|
||||
md5sum: bdce14ae8292bf3deb81f6f255baf859
|
||||
|
||||
- name: tiddit sv no ref
|
||||
command: nextflow run ./tests/modules/tiddit/sv -entry test_tiddit_sv_no_ref -c ./tests/config/nextflow.config -c ./tests/modules/tiddit/sv/nextflow.config
|
||||
|
|
@ -21,3 +22,4 @@
|
|||
- path: output/tiddit/test.signals.tab
|
||||
md5sum: dab4b2fec4ddf8eb1c23005b0770150e
|
||||
- path: output/tiddit/test.vcf
|
||||
md5sum: 3d0e83a8199b2bdb81cfe3e6b12bf64b
|
||||
|
|
|
|||
27
tests/subworkflows/nf-core/bam_qc_picard/main.nf
Normal file
27
tests/subworkflows/nf-core/bam_qc_picard/main.nf
Normal file
|
|
@ -0,0 +1,27 @@
|
|||
#!/usr/bin/env nextflow
|
||||
|
||||
nextflow.enable.dsl = 2
|
||||
|
||||
include { BAM_QC_PICARD } from '../../../../subworkflows/nf-core/bam_qc_picard/main' addParams([:])
|
||||
|
||||
workflow test_bam_qc_picard_wgs {
|
||||
input = [ [ id:'test', single_end:false ], // meta map
|
||||
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
|
||||
]
|
||||
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
||||
fasta_fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
|
||||
|
||||
BAM_QC_PICARD ( input, fasta, fasta_fai, [], [] )
|
||||
}
|
||||
|
||||
workflow test_bam_qc_picard_targetted {
|
||||
input = [ [ id:'test', single_end:false ], // meta map
|
||||
file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true)
|
||||
]
|
||||
fasta = file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true)
|
||||
fasta_fai = file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true)
|
||||
bait = file(params.test_data['sarscov2']['genome']['baits_interval_list'], checkIfExists: true)
|
||||
target = file(params.test_data['sarscov2']['genome']['targets_interval_list'], checkIfExists: true)
|
||||
|
||||
BAM_QC_PICARD ( input, fasta, fasta_fai, bait, target )
|
||||
}
|
||||
5
tests/subworkflows/nf-core/bam_qc_picard/nextflow.config
Normal file
5
tests/subworkflows/nf-core/bam_qc_picard/nextflow.config
Normal file
|
|
@ -0,0 +1,5 @@
|
|||
process {
|
||||
|
||||
publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" }
|
||||
|
||||
}
|
||||
33
tests/subworkflows/nf-core/bam_qc_picard/test.yml
Normal file
33
tests/subworkflows/nf-core/bam_qc_picard/test.yml
Normal file
|
|
@ -0,0 +1,33 @@
|
|||
- name: bam qc picard wgs
|
||||
command: nextflow run ./tests/subworkflows/nf-core/bam_qc_picard -entry test_bam_qc_picard_wgs -c tests/config/nextflow.config
|
||||
tags:
|
||||
- subworkflows
|
||||
# - subworkflows/bam_qc_picard
|
||||
# Modules
|
||||
# - picard
|
||||
# - picard/collectmultiplemetrics
|
||||
# - picard/collectwgsmetrics
|
||||
files:
|
||||
- path: ./output/picard/test.CollectMultipleMetrics.alignment_summary_metrics
|
||||
- path: ./output/picard/test.CollectMultipleMetrics.insert_size_metrics
|
||||
- path: ./output/picard/test.CollectMultipleMetrics.base_distribution_by_cycle_metrics
|
||||
- path: ./output/picard/test.CollectMultipleMetrics.quality_by_cycle_metrics
|
||||
- path: ./output/picard/test.CollectMultipleMetrics.quality_distribution_metrics
|
||||
- path: ./output/picard/test.CollectWgsMetrics.coverage_metrics
|
||||
|
||||
- name: bam qc picard targetted
|
||||
command: nextflow run ./tests/subworkflows/nf-core/bam_qc_picard -entry test_bam_qc_picard_targetted -c tests/config/nextflow.config
|
||||
tags:
|
||||
- subworkflows
|
||||
# - subworkflows/bam_qc_picard
|
||||
# Modules
|
||||
# - picard
|
||||
# - picard/collectmultiplemetrics
|
||||
# - picard/collecthsmetrics
|
||||
files:
|
||||
- path: ./output/picard/test.CollectMultipleMetrics.alignment_summary_metrics
|
||||
- path: ./output/picard/test.CollectMultipleMetrics.insert_size_metrics
|
||||
- path: ./output/picard/test.CollectMultipleMetrics.base_distribution_by_cycle_metrics
|
||||
- path: ./output/picard/test.CollectMultipleMetrics.quality_by_cycle_metrics
|
||||
- path: ./output/picard/test.CollectMultipleMetrics.quality_distribution_metrics
|
||||
- path: ./output/picard/test.CollectHsMetrics.coverage_metrics
|
||||
Loading…
Reference in a new issue