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Module bedtools-genomecov added
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software/bedtools/genomecov/Dockerfile
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software/bedtools/genomecov/Dockerfile
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FROM nfcore/base
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LABEL authors="Jose Espinosa-Carrasco" \
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description="Docker image containing all requirements for nf-core/modules/bedtools/genomecov"
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COPY environment.yml /
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RUN conda env create -f /environment.yml && conda clean -a
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ENV PATH /opt/conda/envs/nf-core-bedtools-complementbed/bin:$PATH
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software/bedtools/genomecov/environment.yml
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software/bedtools/genomecov/environment.yml
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# You can use this file to create a conda environment for this pipeline:
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# conda env create -f environment.yml
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name: nf-core-bedtools-genomecov
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channels:
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- conda-forge
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- bioconda
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- defaults
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dependencies:
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- bioconda::bedtools=2.29.2
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software/bedtools/genomecov/main.nf
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software/bedtools/genomecov/main.nf
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process GENOMECOV {
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tag {bam}
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container 'quay.io/biocontainers/bedtools:2.29.2--hc088bd4_0'
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input:
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path (bam)
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path (fasta_sizes)
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val (genomecov_args)
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output:
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stdout()
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script:
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"""
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bedtools genomecov -ibam ${bam} -g ${fasta_sizes} ${genomecov_args}
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"""
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}
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software/bedtools/genomecov/meta.yml
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software/bedtools/genomecov/meta.yml
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name: bedtools genomecov
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description: Returns feature coverage for a given genome in different formats
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keywords:
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- complement
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tools:
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- bedtools:
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description: |
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Bedtools is a software package that provides with a toolset to perform genome arithmetic operations.
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homepage: https://bedtools.readthedocs.io/en/latest/index.html
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documentation: https://bedtools.readthedocs.io/en/latest/index.html
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doi: 10.093/bioinformatics/btq033
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input:
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-
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- input_file:
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type: file
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description: Input genomic coordinates file
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pattern: "*.{bam}"
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- fasta_sizes:
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type: file
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description: Genome chromosome sizes
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pattern: "*.{txt,sizes}"
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output:
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-
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- index:
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type: stdout,file
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description:
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pattern: "stdout,*.{bed,bedGraph}"
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authors:
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- "@JoseEspinosa"
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software/bedtools/genomecov/test/input_data/genome.sizes
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software/bedtools/genomecov/test/input_data/genome.sizes
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chr1 1780869
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software/bedtools/genomecov/test/main.nf
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software/bedtools/genomecov/test/main.nf
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#!/usr/bin/env nextflow
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nextflow.preview.dsl = 2
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params.genomecov_args = '' //'-bg'
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include check_output from '../../../../tests/functions/check_process_outputs.nf' // params(params)
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include GENOMECOV from '../main.nf' params(params)
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// Define input channels
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ch_input = Channel.fromPath('./input_data/JK2067_downsampled_s0.1.bam')
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chrom_sizes = Channel.fromPath('./input_data/genome.sizes')
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// Run the workflow
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workflow {
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GENOMECOV(ch_input, chrom_sizes, params.genomecov_args)
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// .check_output()
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}
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software/bedtools/genomecov/test/nextflow.config
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software/bedtools/genomecov/test/nextflow.config
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docker.enabled = true
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params.outdir = './results'
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