* Update README
* Rename pytest_software.yml to pytest_modules.yml
* Rename main software directory to modules
* Remove deprecated modules
* Rename tests software to modules
* Replace paths for tests in pytest_modules.yml
* Replace software with modules in Github Actions
* Replace software with modules in main.nf tests
* Rename software to modules in test.yml
* testing works but need to fix paths and add exclude list
* edited meta file and removed TODOs
* left over TODOs
* test passed, final push
* resolving initial comments
* Update software/delly/call/main.nf
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* created templates
* updated functions.nf to the one on dev
* Created test main
* Fasta added and docker, conda, singularity passing
* Fixed output version lint
The `last/lastal` submodule takes query sequences to align to a target
index, and optionally takes one set of alignment parameters (including a
score matrix) computed by the `last/train` module for each of the
sequences.
In the previous implementation the sequences and the alignment
parameters were provided in different channels, causing them to be
sometimes desynchronised.
In the patched implementation, `last/lastal` takes a 3-tuple as
input to ensure synchronicity. To produce this tuple in a pipeline,
one can use the `join` command as in the following example.
LAST_TRAIN ( query,
target )
LAST_LASTAL ( query.join(LAST_TRAIN.out.param_file),
target )
In case no parameter file is computed one can pass a dummy file
to the module as follows:
LAST_LASTAL ( query.map { row -> [ row[0], row[1], [] ] },
target )
* Created module template (#558)
* Created meta and implemented main (#558)
* Conda test and lint passing (#558)
* Updated functions.nf to the one on dev (#558)
* Update software/lofreq/filter/main.nf
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
when using the suffix option in the fastqc module it gets added with a "." to the ${meta.id}, this leads to sample name clashing when passing the fastqc output of raw and processed files suffixed with "trim" or "clean" to multiqc.
I suggest to remove the "." this would also be more consistent with the prefix def in most of the other nf-core modules.
##' Ref:
https://nfcore.slack.com/archives/CJRH30T6V/p1625129138043900
* add software/pairtools
* create a branch for pairtools/select
* fix the different output of conda and docker.
* rmove the md5sum for gzip files.
* Update software/pairtools/select/main.nf
Co-authored-by: Jose Espinosa-Carrasco <kadomu@gmail.com>
* update test file and documentation.
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
Co-authored-by: Jose Espinosa-Carrasco <kadomu@gmail.com>
* Update salmon/quant to provide '--libType A' option
* Modify lib_type description to be a string
* Update tests
* lyb_type can be explicitly overwrite
* Update main.nf
* Update main.nf
* Update main.nf
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Update LAST to version 1238.
* Update functions.nf to the latest devel version.
* Update test MD5sums after updating software version.
* Make portable on MacOS
* Allow input alignments to be uncompressed.
While the strategy in this family of modules is to make all inputs and
outputs compressed, this change might be useful to some users.
As of LAST 2138, `last/split` does not allow its input to be compressed.
* Search for .des file, that is guaranteed to be unique.
Some LAST indexes have more than one .bck file and it makes the name
detection crash.
In this commit, I also standardise how the names are detected.
* Use value input channel and optional output channels to handle formats.
As discussed on Slack, it is preferred to use a value input channel
instead of sneaking options through `params.args2` or `params.format`
as we did.
Likewise, optional output channels with clearly labeled format are
preferred to 'catch-all' wildcards.
* Update test data names.
Closes: #459
Thanks: @FriederikeHanssen
(`samtools` tests are already fixed by another commit)
* Fix file names in tests.
The update of test data name required changes in the test yml files.
I also updated the `bismark/summary/main.nf` file to get its data
from the `params.test_data` map.
* hifiasm copied from fastqc
* hifiasm tests init from fastqc
* meta.yml init; test.yml and main.nf for printing version
* Add hifiasm version printing
* Removed spaced on an empty line
* Reverted hifiasm from main
* hifiasm copied from fastqc
* hifiasm tests init from fastqc
* meta.yml init; test.yml and main.nf for printing version
* Add hifiasm version printing
* Removed spaced on an empty line
* Reverted hifiasm from main
* Added seqtk/subseq and checking for seed in seqtk/sample
* Separate authors in software/seqtk/sample/meta.yml
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Align commans in output channesl software/seqtk/subseq/main.nf
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Define prefix in software/seqtk/subseq/main.nf
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Use prefix in output file name software/seqtk/subseq/main.nf
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Define suffix in options in tests/software/seqtk/subseq/main.nf
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Change output file name in tests/software/seqtk/subseq/test.yml
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Remove a to-do point from tests/software/seqtk/subseq/test.yml
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Added --no-name into gzip commands
* Update samtools from 1.10 to 1.12 (#530)
* feat: remove social preview image to use GitHub OpenGraph
* feat: update samtools from 1.10 to 1.12
* fix: CI tests
* fix: add meta.yml file for samtools/merge
* Update software/samtools/merge/meta.yml
Co-authored-by: Jose Espinosa-Carrasco <kadomu@gmail.com>
* Update software/samtools/merge/meta.yml
Co-authored-by: Jose Espinosa-Carrasco <kadomu@gmail.com>
* hifiasm copied from fastqc
* hifiasm tests init from fastqc
* meta.yml init; test.yml and main.nf for printing version
* Add hifiasm version printing
* Removed spaced on an empty line
* Reverted hifiasm from main
* Added seqtk/subseq and checking for seed in seqtk/sample
* hifiasm copied from fastqc
* hifiasm tests init from fastqc
* meta.yml init; test.yml and main.nf for printing version
* Add hifiasm version printing
* Removed spaced on an empty line
* Reverted hifiasm from main
* Separate authors in software/seqtk/sample/meta.yml
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Align commans in output channesl software/seqtk/subseq/main.nf
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Define prefix in software/seqtk/subseq/main.nf
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Use prefix in output file name software/seqtk/subseq/main.nf
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Define suffix in options in tests/software/seqtk/subseq/main.nf
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Change output file name in tests/software/seqtk/subseq/test.yml
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Remove a to-do point from tests/software/seqtk/subseq/test.yml
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Added --no-name into gzip commands
* Replaced functions.nf in seqtk/subseq
* Refreshed tests for sample and subseq
* Corrected paired-end test and YAML description for sample
Co-authored-by: Sviatoslav Sidorov <sviatoslav.sidorov@crick.ac.uk>
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
Co-authored-by: Maxime U. Garcia <max.u.garcia@gmail.com>
Co-authored-by: Jose Espinosa-Carrasco <kadomu@gmail.com>
* add software/pairtools
* create a branch for pairtools/restrict
* fix the different output of conda and docker
* remove customized code.
* add newline to Frag.bed file.
* change the folder of frag.bed.
* change \n to \r\n
* Remove work.frag.bed
Co-authored-by: JoseEspinosa <kadomu@gmail.com>
* New last/mafconvert module to convert MAF alignments.
The `maf-convert` tool distributed with [LAST](https://gitlab.com/mcfrith/last)
reads alignmnts in [MAF](https://genome-asia.ucsc.edu/FAQ/FAQformat.html#format5)
format and converts them in another format (axt, blast, blasttab, chain,
gff, html, psl, sam, tab).
This new module is part of the work described in Issue #464. During this
development, we fix the versiob of LAST to 1219 to ensure consistency.
We will upgrade it later.
* Delete white space.
* Update the function.nf file to the dev version.
The `last-postmask` tool distributed with [LAST](https://gitlab.com/mcfrith/last)
filters alignments in a MAF file to remove those with too many masked
(lower-case) positions compared with their score.
As other filter modules like `last/split`, its output file risks to
overwrite its input file as their names are constructed from the sample
ID when multiple filters are chained in the pipeline. I added a check
that gives a clearer error message in this case. Please let me know
what you think about; I can add this test to the existing LAST modules
as well.
This new module is part of the work discribed in Issue #464. During this
development, we fix the version of LAST to 1219 to ensure consistency.
We will upgrade it later.
* New last/dotplot module for pairwise similarity plots
The `last-dotplot` tool takes a pairwise alignment in
[MAF](http://genome.ucsc.edu/FAQ/FAQformat.html#format5) format,
possibly compressed with gzip, or in a tabular format produced by the
`maf-convert` tool, and produces a similarity dot-plot of the two
sequences in one of the graphical formats supported by the Python
Imaging Library.
A the tool guesses the output format by the file extension of the file,
which is constructed by the module at run time, I have used the `args2`
option to convey this information to the module.
This new module is part of the work described in Issue #464. During
this development, we fix the version of LAST to 1219 to ensure
consistency (hence please ignore lint's version warning).
* Update the functions.nf file to the dev branch.
https://raw.githubusercontent.com/nf-core/tools/dev/nf_core/module-template/software/functions.nf
* feat: remove social preview image to use GitHub OpenGraph
* feat: update samtools from 1.10 to 1.12
* fix: CI tests
* fix: add meta.yml file for samtools/merge
* Update software/samtools/merge/meta.yml
Co-authored-by: Jose Espinosa-Carrasco <kadomu@gmail.com>
* Update software/samtools/merge/meta.yml
Co-authored-by: Jose Espinosa-Carrasco <kadomu@gmail.com>
* add software/pairtools
* create a branch for pairtools/sort
* fix the different output of conda and docker.
* remove customized code.
* Apply suggestions from code review
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* add software/pairtools
* create a branch for pairtools/parse
* fix the issue of bioconda output is different from docker.
* remove customized code from test.
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* add software/pairtools
* create a branch for pairtools/flip
* fix the issue of PG line in output
* remove custom code from test.
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* New last/lastal to align query sequences on a target index
`lastal` is the main program of the [LAST](https://gitlab.com/mcfrith/last)
suite. It align query DNA sequences in FASTA or FASTQ format to a
target index of DNA or protein sequences. The index is produced by
the `lastdb` program (module `last/lastdb`). The score matrix for
evaluating the alignment can be chosen among preset ones or computed
iteratively by the `last-train` program (module `last/train`). For
this reason, the `last/lastal` module proposed here has one input
channel containing an optional file, that has to be dummy when not used.
The LAST aligner outputs MAF files that can be very large (up to
hundreds of gigabytes), therefore this module unconditionally compresses
its output with gzip.
This new module is part of the work described in Issue #464. During
this development, we fix the version of LAST to 1219 to ensure
consistency (hence ignore lint's version warning).
* Apply suggestions from code review
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
* Un-hardcode the path to the LAST index.
Among multiple alternatives I have chosen the following command to
detect the sample name of the index, because it fails in situations
where there is no index files in the index folder, and in situations
were there are two indexes files in the folder. Not failing would
result in feeding garbage information in the INDEX_NAME variable.
basename \$(ls $index/*.bck) .bck
In case of missing file, a clear error message is given by `ls`. In
case of more than one file, the error message of `basename` is more
cryptic, unfortunately. (`basename: extra operand ‘.bck’`)
Alternatives that do not fail if there is no .bck file:
basename $index/*bck .bck
find $index -name '*bck' | sed 's/.bck//'
Alternatives that do not fail if there are more than one .bck file:
basename -s .bck $index/*bck
ls $index/*.bck | xargs basename -s .bck
find $index -name '*bck' | sed 's/.bck//'
Co-authored-by: Harshil Patel <drpatelh@users.noreply.github.com>
