nf-core_modules/modules/ivar/consensus/main.nf

48 lines
1.6 KiB
Text
Raw Normal View History

2021-02-09 13:26:35 -05:00
// Import generic module functions
include { initOptions; saveFiles; getSoftwareName } from './functions'
params.options = [:]
options = initOptions(params.options)
2021-02-09 13:26:35 -05:00
process IVAR_CONSENSUS {
tag "$meta.id"
label 'process_medium'
publishDir "${params.outdir}",
mode: params.publish_dir_mode,
saveAs: { filename -> saveFiles(filename:filename, options:params.options, publish_dir:getSoftwareName(task.process), meta:meta, publish_by_meta:['id']) }
2021-02-09 13:26:35 -05:00
conda (params.enable_conda ? "bioconda::ivar=1.3.1" : null)
2021-02-09 13:26:35 -05:00
if (workflow.containerEngine == 'singularity' && !params.singularity_pull_docker_container) {
container "https://depot.galaxyproject.org/singularity/ivar:1.3.1--h089eab3_0"
} else {
container "quay.io/biocontainers/ivar:1.3.1--h089eab3_0"
}
input:
tuple val(meta), path(bam)
2021-02-12 18:01:53 -05:00
path fasta
2021-02-09 13:26:35 -05:00
output:
tuple val(meta), path("*.fa") , emit: fasta
tuple val(meta), path("*.qual.txt"), emit: qual
tuple val(meta), path("*.mpileup") , optional:true, emit: mpileup
2021-02-09 13:26:35 -05:00
path "*.version.txt" , emit: version
script:
def software = getSoftwareName(task.process)
def prefix = options.suffix ? "${meta.id}${options.suffix}" : "${meta.id}"
def save_mpileup = params.save_mpileup ? "tee ${prefix}.mpileup |" : ""
2021-02-09 13:26:35 -05:00
"""
samtools mpileup \\
2021-02-23 15:36:03 -05:00
--reference $fasta \\
2021-02-09 13:26:35 -05:00
$options.args2 \\
$bam | \\
$save_mpileup \\
2021-02-09 13:26:35 -05:00
ivar consensus \\
2021-02-12 17:57:06 -05:00
$options.args \\
-p $prefix
2021-02-09 13:26:35 -05:00
2021-03-15 08:21:21 -04:00
echo \$(ivar version 2>&1) | sed 's/^.*iVar version //; s/ .*\$//' > ${software}.version.txt
2021-02-09 13:26:35 -05:00
"""
}