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taxprofiler/conf/modules.config

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/*
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Config file for defining DSL2 per module options and publishing paths
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Available keys to override module options:
ext.args = Additional arguments appended to command in module.
ext.args2 = Second set of arguments appended to command in module (multi-tool modules).
ext.args3 = Third set of arguments appended to command in module (multi-tool modules).
ext.prefix = File name prefix for output files.
----------------------------------------------------------------------------------------
*/
process {
withName: FASTQC {
ext.args = '--quiet'
ext.prefix = { "${meta.id}_${meta.run_accession}_raw" }
publishDir = [
path: { "${params.outdir}/fastqc/raw" },
mode: params.publish_dir_mode,
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pattern: '*.{html,zip}'
]
}
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withName: FASTQC_PROCESSED {
ext.args = '--quiet'
ext.prefix = { "${meta.id}_${meta.run_accession}_processed" }
publishDir = [
path: { "${params.outdir}/fastqc/processed" },
mode: params.publish_dir_mode,
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pattern: '*.{html,zip}'
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]
}
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withName: FALCO {
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ext.prefix = { "${meta.id}_${meta.run_accession}_raw_falco" }
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publishDir = [
path: { "${params.outdir}/falco/raw" },
mode: params.publish_dir_mode,
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pattern: '*.{html,txt,zip}'
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]
}
withName: FALCO_PROCESSED {
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ext.prefix = { "${meta.id}_${meta.run_accession}_processed_falco" }
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publishDir = [
path: { "${params.outdir}/falco/processed" },
mode: params.publish_dir_mode,
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pattern: '*.{html,txt,zip}'
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]
}
withName: FASTP_SINGLE {
ext.args = [
// trimming options
params.shortread_qc_skipadaptertrim ? "--disable_adapter_trimming" : "",
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params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter1 ? "--adapter_sequence ${params.shortread_qc_adapter1}" : "",
// filtering options
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"--length_required ${params.shortread_qc_minlength}",
(params.perform_shortread_complexityfilter && params.shortread_complexityfilter_tool == 'fastp') ? "--low_complexity_filter --complexity_threshold ${params.shortread_complexityfilter_fastp_threshold}" : ''
].join(' ').trim()
ext.prefix = { "${meta.id}_${meta.run_accession}" }
publishDir = [
[
path: { "${params.outdir}/fastp" },
mode: params.publish_dir_mode,
pattern: '*.fastq.gz',
enabled: params.save_preprocessed_reads
],
[
path: { "${params.outdir}/fastp" },
mode: params.publish_dir_mode,
pattern: '*.{log,html,json}'
],
[
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path: { "${params.outdir}/analysis_ready_fastqs" },
mode: params.publish_dir_mode,
pattern: '*.fastq.gz',
enabled: params.save_analysis_ready_fastqs,
// Don't know why `!` doesn't work here, but `== false` makes it work...
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saveAs: { ( params.perform_runmerging == false || ( params.perform_runmerging && !meta.is_multirun ) ) && !params.perform_shortread_hostremoval && !params.perform_shortread_complexityfilter && params.perform_shortread_qc && params.save_analysis_ready_fastqs ? it : null }
]
]
}
withName: FASTP_PAIRED {
ext.args = [
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// collapsing options - option to retain singletons
params.shortread_qc_includeunmerged ? '--include_unmerged' : '',
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// trimming options
params.shortread_qc_skipadaptertrim ? "--disable_adapter_trimming" : "",
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params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter1 ? "--adapter_sequence ${params.shortread_qc_adapter1}" : "",
params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter2 ? "--adapter_sequence_r2 ${params.shortread_qc_adapter2}" : "--detect_adapter_for_pe",
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// filtering options
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"--length_required ${params.shortread_qc_minlength}",
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params.perform_shortread_complexityfilter && params.shortread_complexityfilter_tool == 'fastp' ? "--low_complexity_filter --complexity_threshold ${params.shortread_complexityfilter_fastp_threshold}" : ''
].join(' ').trim()
ext.prefix = { "${meta.id}_${meta.run_accession}" }
publishDir = [
[
path: { "${params.outdir}/fastp" },
mode: params.publish_dir_mode,
pattern: '*.fastq.gz',
enabled: params.save_preprocessed_reads
],
[
path: { "${params.outdir}/fastp" },
mode: params.publish_dir_mode,
pattern: '*.{log,html,json}'
],
[
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path: { "${params.outdir}/analysis_ready_fastqs" },
mode: params.publish_dir_mode,
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pattern: params.shortread_qc_mergepairs ? '*merged.fastq.gz' : '*.fastp.fastq.gz',
enabled: params.save_analysis_ready_fastqs,
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saveAs: { ( params.perform_runmerging == false || ( params.perform_runmerging && !meta.is_multirun ) ) && !params.perform_shortread_hostremoval && !params.perform_shortread_complexityfilter && params.perform_shortread_qc && params.save_analysis_ready_fastqs ? it : null }
]
]
}
withName: ADAPTERREMOVAL_SINGLE {
ext.args = [
// trimming options
params.shortread_qc_skipadaptertrim ? "--adapter1 ''" : params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter1 ? "--adapter1 ${params.shortread_qc_adapter1}" : "",
// filtering options
"--minlength ${params.shortread_qc_minlength}"
].join(' ').trim()
ext.prefix = { "${meta.id}_${meta.run_accession}" }
publishDir = [
[
path: { "${params.outdir}/adapterremoval" },
mode: params.publish_dir_mode,
pattern: '*.fastq.gz',
enabled: params.save_preprocessed_reads
],
[
path: { "${params.outdir}/adapterremoval" },
mode: params.publish_dir_mode,
pattern: '*.settings'
],
[
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path: { "${params.outdir}/analysis_ready_fastqs" },
mode: params.publish_dir_mode,
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pattern: '*truncated.fastq.gz',
enabled: params.save_analysis_ready_fastqs,
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saveAs: { ( params.perform_runmerging == false || ( params.perform_runmerging && !meta.is_multirun ) ) && !params.perform_shortread_hostremoval && !params.perform_shortread_complexityfilter && params.perform_shortread_qc && params.save_analysis_ready_fastqs ? it : null }
]
]
}
withName: ADAPTERREMOVAL_PAIRED {
ext.args = [
// collapsing options
params.shortread_qc_mergepairs ? "--collapse" : "",
// trimming options
params.shortread_qc_skipadaptertrim ? "--adapter1 ''" : params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter1 ? "--adapter1 ${params.shortread_qc_adapter1}" : "", // adding adapter list happens at module input channel level
params.shortread_qc_skipadaptertrim ? "--adapter2 ''" : params.shortread_qc_adapterlist ? "" : params.shortread_qc_adapter2 ? "--adapter2 ${params.shortread_qc_adapter2}" : "",
// filtering options
"--minlength ${params.shortread_qc_minlength}"
].join(' ').trim()
ext.prefix = { "${meta.id}_${meta.run_accession}" }
publishDir = [
[
path: { "${params.outdir}/adapterremoval" },
mode: params.publish_dir_mode,
pattern: '*.fastq.gz',
enabled: params.save_preprocessed_reads
],
[
path: { "${params.outdir}/adapterremoval" },
mode: params.publish_dir_mode,
pattern: '*.settings'
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],
[
path: { "${params.outdir}/analysis_ready_fastqs" },
mode: params.publish_dir_mode,
pattern: '*{truncated.fastq,singleton.truncated}.gz',
enabled: params.save_analysis_ready_fastqs,
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saveAs: { ( params.perform_runmerging == false || ( params.perform_runmerging && !meta.is_multirun ) ) && !params.perform_shortread_hostremoval && !params.perform_shortread_complexityfilter && params.perform_shortread_qc && !params.shortread_qc_mergepairs && params.save_analysis_ready_fastqs ? it : null}
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]
]
}
// AdapterRemoval separate output merging
withName: CAT_FASTQ {
ext.prefix = { "${meta.id}_${meta.run_accession}" }
publishDir = [
[
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path: { "${params.outdir}/analysis_ready_fastqs" },
mode: params.publish_dir_mode,
pattern: '*.fastq.gz',
enabled: params.save_analysis_ready_fastqs,
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saveAs: { ( params.perform_runmerging == false || ( params.perform_runmerging && !meta.is_multirun ) ) && !params.perform_shortread_hostremoval && !params.perform_shortread_complexityfilter && params.perform_shortread_qc && params.save_analysis_ready_fastqs ? it : null }
]
]
}
withName: PORECHOP_PORECHOP {
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ext.prefix = { "${meta.id}_${meta.run_accession}" }
publishDir = [
[
path: { "${params.outdir}/porechop" },
mode: params.publish_dir_mode,
pattern: '*_porechopped.fastq.gz',
enabled: params.save_preprocessed_reads
],
[
path: { "${params.outdir}/porechop" },
mode: params.publish_dir_mode,
pattern: '*.log'
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],
[
path: { "${params.outdir}/analysis_ready_fastqs" },
mode: params.publish_dir_mode,
pattern: '*_porechopped.fastq.gz',
enabled: params.save_analysis_ready_fastqs,
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saveAs: { ( params.perform_runmerging == false || ( params.perform_runmerging && !meta.is_multirun ) ) && !params.perform_longread_hostremoval && params.longread_qc_skipqualityfilter && !params.longread_qc_skipadaptertrim && params.perform_longread_qc && params.save_analysis_ready_fastqs ? it : null }
]
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]
}
withName: FILTLONG {
ext.args = [
"--min_length ${params.longread_qc_qualityfilter_minlength}",
"--keep_percent ${params.longread_qc_qualityfilter_keeppercent}",
"--target_bases ${params.longread_qc_qualityfilter_targetbases}"
]
.join(' ').trim()
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ext.prefix = { "${meta.id}_${meta.run_accession}_filtered" }
publishDir = [
[
path: { "${params.outdir}/filtlong" },
mode: params.publish_dir_mode,
pattern: '*.fastq.gz',
enabled: params.save_preprocessed_reads
],
[
path: { "${params.outdir}/filtlong" },
mode: params.publish_dir_mode,
pattern: '*.log'
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],
[
path: { "${params.outdir}/analysis_ready_fastqs" },
mode: params.publish_dir_mode,
pattern: '*.fastq.gz',
enabled: params.save_analysis_ready_fastqs,
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saveAs: { ( params.perform_runmerging == false || ( params.perform_runmerging && !meta.is_multirun ) ) && !params.perform_longread_hostremoval && !params.longread_qc_skipqualityfilter && params.perform_longread_qc && params.save_analysis_ready_fastqs ? it : null }
]
]
}
withName: BBMAP_BBDUK {
ext.args = [
"entropy=${params.shortread_complexityfilter_entropy}",
"entropywindow=${params.shortread_complexityfilter_bbduk_windowsize}",
params.shortread_complexityfilter_bbduk_mask ? "entropymask=t" : "entropymask=f"
].join(' ').trim()
ext.prefix = { "${meta.id}_${meta.run_accession}" }
publishDir = [
[
path: { "${params.outdir}/bbduk/" },
mode: params.publish_dir_mode,
pattern: '*.{fastq.gz}',
enabled: params.save_complexityfiltered_reads
],
[
path: { "${params.outdir}/bbduk/" },
mode: params.publish_dir_mode,
pattern: '*.log'
],
[
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path: { "${params.outdir}/analysis_ready_fastqs" },
mode: params.publish_dir_mode,
pattern: '*.fastq.gz',
enabled: params.save_analysis_ready_fastqs,
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saveAs: { ( params.perform_runmerging == false || ( params.perform_runmerging && !meta.is_multirun ) ) && !params.perform_shortread_hostremoval && params.shortread_complexityfilter_tool && params.save_analysis_ready_fastqs ? it : null }
]
]
}
withName: PRINSEQPLUSPLUS {
ext.args = [
params.shortread_complexityfilter_prinseqplusplus_mode == 'dust' ? "-lc_dust=${params.shortread_complexityfilter_prinseqplusplus_dustscore}" : "-lc_entropy=${params.shortread_complexityfilter_entropy}",
"-trim_qual_left=0 -trim_qual_left=0 -trim_qual_window=0 -trim_qual_step=0",
].join(' ').trim()
ext.prefix = { "${meta.id}_${meta.run_accession}" }
publishDir = [
[
path: { "${params.outdir}/prinseqplusplus/" },
mode: params.publish_dir_mode,
pattern: '*{_good_out.fastq.gz,_good_out_R1.fastq.gz,_good_out_R2.fastq.gz}',
enabled: params.save_complexityfiltered_reads
],
[
path: { "${params.outdir}/prinseqplusplus/" },
mode: params.publish_dir_mode,
pattern: '*.log'
],
[
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path: { "${params.outdir}/analysis_ready_fastqs" },
mode: params.publish_dir_mode,
pattern: '*{_good_out.fastq.gz,_good_out_R1.fastq.gz,_good_out_R2.fastq.gz}',
enabled: params.save_analysis_ready_fastqs,
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saveAs: { ( params.perform_runmerging == false || ( params.perform_runmerging && !meta.is_multirun ) ) && !params.perform_shortread_hostremoval && params.shortread_complexityfilter_tool && params.save_analysis_ready_fastqs ? it : null }
]
]
}
withName: BOWTIE2_BUILD {
publishDir = [
[
path: { "${params.outdir}/bowtie2/build" },
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mode: params.publish_dir_mode,
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pattern: 'bowtie2',
enabled: params.save_hostremoval_index
]
]
}
// Saving unmapped reads as FQ comes via input channel!
withName: BOWTIE2_ALIGN {
ext.prefix = { "${meta.id}_${meta.run_accession}" }
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publishDir = [
[
path: { "${params.outdir}/bowtie2/align" },
mode: params.publish_dir_mode,
pattern: '*.log'
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],
[
path: { "${params.outdir}/bowtie2/align" },
mode: params.publish_dir_mode,
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pattern: '*.bam',
enabled: params.save_hostremoval_bam
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],
[
path: { "${params.outdir}/bowtie2/align" },
mode: params.publish_dir_mode,
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pattern: '*.fastq.gz',
enabled: params.save_hostremoval_unmapped
],
[
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path: { "${params.outdir}/analysis_ready_fastqs" },
mode: params.publish_dir_mode,
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enabled: params.perform_shortread_hostremoval,
pattern: '*.fastq.gz',
enabled: params.save_analysis_ready_fastqs,
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saveAs: { ( params.perform_runmerging == false || ( params.perform_runmerging && !meta.is_multirun ) ) && params.perform_shortread_hostremoval && params.save_analysis_ready_fastqs ? it : null }
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]
]
}
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withName: MINIMAP2_INDEX {
ext.args = '-x map-ont'
publishDir = [
path: { "${params.outdir}/minimap2/index" },
mode: params.publish_dir_mode,
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pattern: '*.mmi',
enabled: params.save_hostremoval_index
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]
}
withName: MINIMAP2_ALIGN {
ext.prefix = { "${meta.id}_${meta.run_accession}" }
publishDir = [
path: { "${params.outdir}/minimap2/align" },
mode: params.publish_dir_mode,
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pattern: '*.bam',
enabled: params.save_hostremoval_bam
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]
}
withName: SAMTOOLS_VIEW {
ext.args = '-f 4'
ext.prefix = { "${meta.id}_${meta.run_accession}.unmapped" }
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}
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withName: SAMTOOLS_FASTQ {
ext.prefix = { "${meta.id}_${meta.run_accession}.unmapped" }
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publishDir = [
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[
path: { "${params.outdir}/samtools/fastq" },
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mode: params.publish_dir_mode,
pattern: '*.fastq.gz',
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enabled: params.save_hostremoval_unmapped
],
[
path: { "${params.outdir}/analysis_ready_fastqs" },
mode: params.publish_dir_mode,
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pattern: '*.fq.gz',
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enabled: params.save_analysis_ready_fastqs,
saveAs: { ( params.perform_runmerging == false || ( params.perform_runmerging && !meta.is_multirun) ) && params.perform_longread_hostremoval && params.save_analysis_ready_fastqs ? it : null }
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]
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]
}
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withName: SAMTOOLS_STATS {
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ext.prefix = { "${meta.id}_${meta.run_accession}" }
publishDir = [
path: { "${params.outdir}/samtools/stats" },
mode: params.publish_dir_mode,
pattern: '*stats'
]
}
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withName: MERGE_RUNS {
ext.prefix = { "${meta.id}" }
publishDir = [
[
path: { "${params.outdir}/run_merging/" },
mode: params.publish_dir_mode,
pattern: '*.fastq.gz',
enabled: params.save_runmerged_reads
],
[
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path: { "${params.outdir}/analysis_ready_fastqs" },
mode: params.publish_dir_mode,
pattern: '*.fastq.gz',
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enabled: params.perform_runmerging && params.save_analysis_ready_fastqs
]
]
}
withName: MALT_RUN {
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ext.args = { "${meta.db_params} -m ${params.malt_mode}" }
// one run with multiple samples, so fix ID to just db name to ensure clean log name
ext.prefix = { "${meta.db_name}" }
publishDir = [
path: { "${params.outdir}/malt/${meta.db_name}/" },
mode: params.publish_dir_mode,
pattern: '*.{rma6,log,sam}'
]
}
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withName: 'MEGAN_RMA2INFO_TSV' {
ext.args = "-c2c Taxonomy"
ext.prefix = { "${meta.id}" }
publishDir = [
path: { "${params.outdir}/malt/${meta.db_name}/" },
mode: params.publish_dir_mode,
pattern: '*.{txt.gz,megan}'
]
}
withName: KRAKEN2_KRAKEN2 {
ext.args = params.kraken2_save_minimizers ? { "${meta.db_params} --report-minimizer-data" } : { "${meta.db_params}" }
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ext.prefix = params.perform_runmerging ? { meta.tool == "bracken" ? "${meta.id}_${meta.db_name}.bracken" : "${meta.id}_${meta.db_name}.kraken2" } : { meta.tool == "bracken" ? "${meta.id}_${meta.run_accession}_${meta.db_name}.bracken" : "${meta.id}_${meta.run_accession}_${meta.db_name}.kraken2" }
publishDir = [
path: { "${params.outdir}/kraken2/${meta.db_name}/" },
mode: params.publish_dir_mode,
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pattern: '*.{txt,fastq.gz}'
]
}
withName: BRACKEN_BRACKEN {
ext.args = { "${meta.db_params}" }
ext.prefix = params.perform_runmerging ? { "${meta.id}_${meta.db_name}.bracken" } : { "${meta.id}_${meta.run_accession}_${meta.db_name}.bracken" }
publishDir = [
path: { "${params.outdir}/bracken/${meta.db_name}/" },
mode: params.publish_dir_mode,
pattern: '*.tsv'
]
}
withName: BRACKEN_COMBINEBRACKENOUTPUTS {
ext.prefix = { "bracken_${meta.id}_combined_reports" }
publishDir = [
path: { "${params.outdir}/bracken/" },
mode: params.publish_dir_mode,
pattern: '*.txt'
]
}
withName: KRAKENTOOLS_COMBINEKREPORTS_KRAKEN {
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ext.prefix = { "kraken2_${meta.id}_combined_reports" }
publishDir = [
path: { "${params.outdir}/kraken2/" },
mode: params.publish_dir_mode,
pattern: '*.txt'
]
}
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withName: KRAKENUNIQ_PRELOADEDKRAKENUNIQ {
ext.args = { "${meta.db_params}" }
// one run with multiple samples, so fix ID to just db name to ensure clean log name
ext.prefix = { "${meta.db_name}.krakenuniq" }
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publishDir = [
path: { "${params.outdir}/krakenuniq/${meta.db_name}/" },
mode: params.publish_dir_mode,
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pattern: '*.{txt,fastq.gz}'
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]
}
withName: KRONA_CLEANUP {
ext.prefix = params.perform_runmerging ? { "${meta.id}_${meta.db_name}" } : { "${meta.id}_${meta.run_accession}_${meta.db_name}" }
publishDir = [
path: { "${params.outdir}/krona/" },
mode: params.publish_dir_mode,
pattern: '*.{html}'
]
}
withName: KRONA_KTIMPORTTEXT {
ext.prefix = { "${meta.tool}_${meta.id}" }
publishDir = [
path: { "${params.outdir}/krona/" },
mode: params.publish_dir_mode,
pattern: '*.{html}'
]
}
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withName: 'MEGAN_RMA2INFO_KRONA' {
ext.args = { "--read2class Taxonomy" }
ext.prefix = { "${meta.id}_${meta.db_name}" }
}
withName: KRONA_KTIMPORTTAXONOMY {
ext.args = "-i"
ext.prefix = { "${meta.tool}_${meta.id}" }
publishDir = [
path: { "${params.outdir}/krona/" },
mode: params.publish_dir_mode,
pattern: '*.{html}'
]
}
withName: METAPHLAN3_METAPHLAN3 {
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ext.args = { "${meta.db_params}" }
ext.prefix = params.perform_runmerging ? { "${meta.id}_${meta.db_name}.metaphlan3" } : { "${meta.id}_${meta.run_accession}_${meta.db_name}.metaphlan3" }
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publishDir = [
path: { "${params.outdir}/metaphlan3/${meta.db_name}/" },
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mode: params.publish_dir_mode,
pattern: '*.{biom,txt}'
]
}
withName: METAPHLAN3_MERGEMETAPHLANTABLES {
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ext.prefix = { "metaphlan3_${meta.id}_combined_reports" }
publishDir = [
path: { "${params.outdir}/metaphlan3/" },
mode: params.publish_dir_mode,
pattern: '*.{txt}'
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]
}
withName: CENTRIFUGE_CENTRIFUGE {
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ext.args = { "${meta.db_params}" }
ext.prefix = params.perform_runmerging ? { "${meta.id}_${meta.db_name}.centrifuge" } : { "${meta.id}_${meta.run_accession}_${meta.db_name}.centrifuge" }
publishDir = [
path: { "${params.outdir}/centrifuge/${meta.db_name}/" },
mode: params.publish_dir_mode,
pattern: '*.{txt,sam,gz}'
]
}
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withName: CENTRIFUGE_KREPORT {
ext.args = { "${meta.db_params}" }
ext.prefix = params.perform_runmerging ? { "${meta.id}_${meta.db_name}.centrifuge" } : { "${meta.id}_${meta.run_accession}_${meta.db_name}.centrifuge" }
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publishDir = [
path: { "${params.outdir}/centrifuge/${meta.db_name}/" },
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mode: params.publish_dir_mode,
pattern: '*.{txt}'
]
}
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withName: KRAKENTOOLS_COMBINEKREPORTS_CENTRIFUGE {
ext.prefix = { "centrifuge_${meta.id}_combined_reports" }
publishDir = [
path: { "${params.outdir}/centrifuge/" },
mode: params.publish_dir_mode,
pattern: '*.{txt}'
]
}
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withName: KAIJU_KAIJU {
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ext.args = { "${meta.db_params}" }
ext.prefix = params.perform_runmerging ? { "${meta.id}_${meta.db_name}.kaiju" } : { "${meta.id}_${meta.run_accession}_${meta.db_name}.kaiju" }
publishDir = [
path: { "${params.outdir}/kaiju/${meta.db_name}/" },
mode: params.publish_dir_mode,
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pattern: '*.tsv'
]
}
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withName: 'KAIJU_KAIJU2TABLE_SINGLE' {
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ext.prefix = params.perform_runmerging ? { "${meta.id}_${meta.db_name}.kaijutable" } : { "${meta.id}_${meta.run_accession}_${meta.db_name}.kaijutable" }
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publishDir = [
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path: { "${params.outdir}/kaiju/${meta.db_name}/" },
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mode: params.publish_dir_mode,
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pattern: '*.{txt}'
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]
}
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withName: 'KAIJU_KAIJU2TABLE_COMBINED' {
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ext.prefix = { "kaiju_${meta.id}_combined_reports" }
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publishDir = [
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path: { "${params.outdir}/kaiju/" },
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mode: params.publish_dir_mode,
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pattern: '*.{txt}'
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]
}
withName: KAIJU_KAIJU2KRONA {
ext.args = '-v -u'
}
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withName: DIAMOND_BLASTX {
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ext.args = { "${meta.db_params}" }
ext.prefix = params.perform_runmerging ? { "${meta.id}_${meta.db_name}.diamond" } : { "${meta.id}_${meta.run_accession}_${meta.db_name}.diamond" }
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publishDir = [
path: { "${params.outdir}/diamond/${meta.db_name}/" },
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mode: params.publish_dir_mode,
pattern: '*.{blast,xml,txt,daa,sam,tsv,paf,log}'
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]
}
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withName: MOTUS_PROFILE {
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ext.args = {
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[
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params.motus_remove_ncbi_ids ? "" : "-p",
params.motus_use_relative_abundance ? "" : "-c",
params.motus_save_mgc_read_counts ? "-M ${task.ext.prefix}.mgc" : ""
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].join(',').replaceAll(','," ")
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}
ext.prefix = params.perform_runmerging ? { "${meta.id}_${meta.db_name}" } : { "${meta.id}_${meta.run_accession}_${meta.db_name}" }
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publishDir = [
path: { "${params.outdir}/motus/${meta.db_name}/" },
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mode: params.publish_dir_mode
]
}
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withName: MOTUS_MERGE {
ext.args = { params.standardisation_motus_generatebiom ? "-B" : "" }
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ext.prefix = { "motus_${meta.id}_combined_reports" }
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publishDir = [
path: { "${params.outdir}/motus/" },
mode: params.publish_dir_mode
]
}
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withName: TAXPASTA_MERGE {
ext.args = {
[
"-p ${meta.tool} -o ${meta.tool}_${meta.id}.${params.standardisation_taxpasta_format}",
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params.taxpasta_taxonomy_dir ? "--taxonomy ${params.taxpasta_taxonomy_dir}" : "",
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params.taxpasta_add_name ? "--add-name" : "",
params.taxpasta_add_rank ? "--add-rank" : "",
params.taxpasta_add_lineage ? "--add-lineage" : "",
params.taxpasta_add_idlineage ? "--add-id-lineage" : ""
].join(' ').trim()
}
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publishDir = [
path: { "${params.outdir}/taxpasta/" },
mode: params.publish_dir_mode,
pattern: '*.{tsv,csv,arrow,parquet,biom}'
]
}
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withName: CUSTOM_DUMPSOFTWAREVERSIONS {
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publishDir = [
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path: { "${params.outdir}/pipeline_info" },
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mode: params.publish_dir_mode,
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pattern: '*_versions.yml'
]
}
withName: MULTIQC {
publishDir = [
path: { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" },
mode: params.publish_dir_mode,
saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
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]
}
}